食品与发酵工业 >

2022 , Vol. 48 >Issue 10: 9 - 15

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.029685

研究报告

黑曲霉脂肪酶tabI酶学性质及乳酸乙酯合成能力的研究

  • 刘小敏 ,
  • 谢翔云 ,
  • 林良才 ,
  • 张煜行 ,
  • 肖冬光
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  • 1(天津科技大学 生物工程学院,天津,300457)
    2(河北衡水老白干酿酒(集团)有限公司,河北 衡水,053000)
第一作者:硕士研究生(肖冬光教授为通信作者,E-mail:xiao99@tust.edu.cn)

收稿日期: 2021-10-12

  修回日期: 2021-11-11

  网络出版日期: 2022-06-10

基金资助

国家重点研发计划(2016YFD04005)

收起

Enzymatic properties of Aspergillus niger lipase tabI and its synthesis ability of ethyl lactate

  • LIU Xiaomin ,
  • XIE Xiangyun ,
  • LIN Liangcai ,
  • ZHANG Yuhang ,
  • XIAO Dongguang
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  • 1(School of Biological Engineering, Tianjin University of Science and Technology, Tianjin 300457, China)
    2(Hebei Hengshui Laobaigan Liquor Group Co.Ltd., Hengshui 053000, China)

Received date: 2021-10-12

  Revised date: 2021-11-11

  Online published: 2022-06-10

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摘要

乳酸乙酯是白酒的重要风味物质。该研究通过转录组分析得到了在黑曲霉H7中基因转录水平较高且功能未知的新型脂肪酶基因tabI,并成功在大肠杆菌BL21(DE3)中异源表达。对其进行酶学性质解析可知,脂肪酶tabI在40 ℃、pH 8.0时表现出最高酶活力,以对硝基苯酚乙酸酯为底物测得该酶的Km值为0.85 mmol/L,Vmax值为63.75 mmol/(mL·h)。对脂肪酶tabI合成乳酸乙酯的条件进行了优化,反应12 h乳酸乙酯的合成量达210.24 mg/L,表明该酶具有合成乳酸乙酯的能力,此结果为乳酸乙酯脂肪酶的开发打下了基础。

关键词： 黑曲霉; 脂肪酶; 转录组分析; 酶活力; 乳酸乙酯

本文引用格式

刘小敏 , 谢翔云 , 林良才 , 张煜行 , 肖冬光 . 黑曲霉脂肪酶tabI酶学性质及乳酸乙酯合成能力的研究[J]. 食品与发酵工业, 2022 , 48(10) : 9 -15 . DOI: 10.13995/j.cnki.11-1802/ts.029685

Abstract

Ethyl lactate is an important flavor substance in liquor. In this study, tabI, a new type of lipase gene, which has high transcription level and unknown function in Aspergillus niger H7, was obtained through transcriptome analysis, and it was heterogeneous expressed in Escherichia coli BL21(DE3). The analysis of its enzymatic properties showed that the highest enzyme activity was obtained at 40 ℃ and pH 8.0. Using 4-nitrophenyl acetate as substrate, the Km value was 0.85mmol/L and the Vmax was 63.75mmol/(mL·h). After optimization of the ethyl lactate synthetic conditions, the yield reached to 210.24 mg/L after 12 h. Above showed that tabI had the ability of synthesizing ethyl lactate, meanwhile the result laid the foundation for the deeper utilizing of ethyl lactate lipase.

Key words： Aspergillus niger; lipase; transcriptome analysis; enzyme activity; ethyl lactate

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