食品与发酵工业 >

2022 , Vol. 48 >Issue 10: 101 - 105

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.028844

研究报告

黄连对肠道致病菌的体外抑菌作用

  • 蒋丽施 ,
  • 左栖枫 ,
  • 张新明 ,
  • 罗园 ,
  • 李新爱 ,
  • 郭文秀 ,
  • 邓赟
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  • 1(成都中医药大学 药学院,四川 成都,611100)
    2(成都中医药大学 公共卫生学院,四川 成都,611100)
    3(成都中医药大学 医学技术学院,四川 成都,611100)
第一作者:硕士,讲师(邓赟研究员为通信作者,E-mail:dengyun2000@hotmail.com)

收稿日期: 2021-08-10

  修回日期: 2021-09-28

  网络出版日期: 2022-06-10

基金资助

国家自然科学基金联合基金项目(U19A200061);成都中医药大学一流学科建设项目(CZYJC1905);成都中医药大学“杏林人才提升计划”(ZRQN2020029)

收起

Bacteriostasis of Coptis on intestinal pathogens in vitro

  • JIANG Lishi ,
  • ZUO Xifeng ,
  • ZHANG Xinming ,
  • LUO Yuan ,
  • LI Xinai ,
  • GUO Wenxiu ,
  • DENG Yun
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  • 1(College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611100, China)
    2(College of Public Health, Chengdu University of Traditional Chinese Medicine, Chengdu 611100, China)
    3(College of Medical Technology, Chengdu University of Traditional Chinese Medicine, Chengdu 611100, China)

Received date: 2021-08-10

  Revised date: 2021-09-28

  Online published: 2022-06-10

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摘要

黄连具有显著的抑菌活性,在天然食品防腐保鲜剂的开发方面潜力巨大。但不同品种、炮制前后的黄连对食品中常见的肠道致病菌的抑菌作用研究未见报道,药效物质基础尚不完全清楚。采用70%甲醇超声辅助提取分别得到味连、雅连生品及酒制品的甲醇提取物(methanol extracts,ME),再依次萃取得到4种中药饮片的石油醚部位(petroleum ether extract,PE)、乙酸乙酯部位(ethyl acetate extract,EAE)、正丁醇部位(n-butanol extract,BE)和水部位(water extract,WE)的浸膏。采用滤纸片法测定不同极性部位浸膏的抑菌活性,并采用二倍稀释法测定最小抑菌浓度(minimum inhibitory concentration,MIC)。实验结果表明,味连对金黄色葡萄球菌、耶尔森氏菌的抑菌效果更佳,而雅连对阴沟肠杆菌抑菌作用更强。酒制雅连和味连对肺炎克雷伯氏菌抑菌活性均明显增强。4种中药饮片的抑菌活性最强的部位为ME,其次为BE和WE部位。黄连对供试细菌的MIC为3.125~25 mg/mL。黄连品种、炮制对黄连的抑菌活性均有一定影响。

关键词： 味连; 雅连; 炮制品; 极性部位; 肠道致病菌

本文引用格式

蒋丽施 , 左栖枫 , 张新明 , 罗园 , 李新爱 , 郭文秀 , 邓赟 . 黄连对肠道致病菌的体外抑菌作用[J]. 食品与发酵工业, 2022 , 48(10) : 101 -105 . DOI: 10.13995/j.cnki.11-1802/ts.028844

Abstract

Coptis herbs has significant antibacterial activity and has great potential in the development of natural food preservatives. However, there has been no report on the anti-bacteriostasis of different varieties and processed Coptis on common intestinal pathogens in food, and the pharmacodynamic material basis is not completely clear. The methanol extracts(ME)of raw and wine-processed Coptis chinensis Franch. and Coptis deltoidea C.Y. Cheng et Hisao were obtained by ultrasonic-assisted extraction with 70% methanol, and then the petroleum ether extract (PE), ethyl acetate extract (EAE), n-butanol extract (BE), water extract (WE) of the four herbs were extracted in turn. The antibacterial activity of extracts from different polar parts was determined by filter paper method, and the minimum inhibitory concentration (MIC) was determined by double dilution method. The experimental results showed that C. chinensis had a better inhibitory effect on Staphylococcus aureus and Yersinia enterocolitica, while C. deltoidea had a stronger inhibitory effect on Enterobacter cloacae. The antibacterial activity of both wine-processed Coptis herbs against Klebsiella pneumoniae was significantly enhanced. The strongest antibacterial activity of Coptis herbs was methanol extract, followed by n-butanol extract and water extract. The MIC value of Coptis herbs ranged from 3.125 to 25 mg/mL. The variety and processing had certain influence on the antibacterial activity of Coptis.

Key words： Coptis chinensis; Coptis deltoidea; processed products; polarity part; enteropathogenic bacteria

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