食品与发酵工业 >

2022 , Vol. 48 >Issue 10: 119 - 125

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.029063

研究报告

六株植物乳杆菌的益生特性研究

  • 郑越 ,
  • 段涛 ,
  • 宋丹 ,
  • 乔琳 ,
  • 陈丽仙 ,
  • 王薇薇 ,
  • 宾石玉
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  • 1(广西师范大学 生命科学学院,广西 桂林,541061)
    2(国家粮食和物资储备局科学研究院,北京,100037)
第一作者:硕士研究生(王薇薇副研究员和宾石玉教授为共同通信作者,E-mail:www@ags.ac.cn;binsy@gxnu.edu.cn)

收稿日期: 2021-08-18

  修回日期: 2021-09-23

  网络出版日期: 2022-06-10

基金资助

广东省重点领域研发计划项目(2020B020201001);中央级公益性科研院所基本科研业务费专项(JY2015;JY2016;ZX1924)

收起

Probiotic properties of six Lactobacillus plantarum strains

  • ZHENG Yue ,
  • DUAN Tao ,
  • SONG Dan ,
  • QIAO Lin ,
  • CHEN Lixian ,
  • WANG Weiwei ,
  • BIN Shiyu
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  • 1(College of life science, Guangxi Normal University, Guilin 541061, China)
    2(Academy of National Food and Strategic Reserves Administration, Beijing 100037, China)

Received date: 2021-08-18

  Revised date: 2021-09-23

  Online published: 2022-06-10

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摘要

该试验旨在研究不同来源的6株植物乳杆菌(分别为LP15-1、LP15-2、LC16-1、LP17-1、LP17-3、RS048)的益生特性。试验通过测定菌株的耐酸耐胆盐能力、模拟胃肠道耐受能力、抑制有害菌能力分别对结肠上皮细胞系Caco-2和空肠上皮细胞系IPEC-1细胞的黏附性等特性,考察植物乳杆菌的益生特性。研究结果表明,6株植物乳杆菌在pH=2的酸环境中孵育3 h存活率均在43%以上;除LC16-1和LP17-1外,其余4株均能在0.3%(质量分数)的胆盐中存活;在模拟胃肠液中存活率均高于95%,对金黄色葡萄球菌、鼠伤寒沙门氏菌、大肠杆菌K88和大肠杆菌K99有较好的抑制能力;对Caco-2和IPEC-1有一定的黏附能力。其中植物乳杆菌LP15-1各项益生特性均比较优良,可作为潜在的益生菌株进行进一步研究和应用。

关键词： 植物乳杆菌; 益生特性; 耐酸; 抑菌能力; 黏附能力

本文引用格式

郑越 , 段涛 , 宋丹 , 乔琳 , 陈丽仙 , 王薇薇 , 宾石玉 . 六株植物乳杆菌的益生特性研究[J]. 食品与发酵工业, 2022 , 48(10) : 119 -125 . DOI: 10.13995/j.cnki.11-1802/ts.029063

Abstract

This study was aimed to study the probiotic properties of six strains of Lactobacillus plantarum (LP15-1, LP15-2, LC16-1, LP17-1, LP17-3, RS048) isolated from different sources. The probiotic properties of Lactobacillus plantarum were investigated by measuring the tolerance ability against acid, bile salt and simulated gastrointestinal fluid, their bacteriostasis, and the adhesion ability to intestinal epithelial cells (Caco-2 and IPEC-1 cells). The survival rate of all six strains incubating under acid (pH 2.0) for 3 h was above 43 %; except for LC16-1 and LP17-1, the other four strains could survive in 0.3 % bile salt. However, the survival rates of the six strains were all above 95 % in simulated gastrointestinal fluid cultured for 3 h. All six strains had good inhibitory ability against pernicious bacteria, such as Staphylococcus aureus, Salmonella typhimurium, Escherichia coli K88 and Escherichia coli K99. In addition, certain adhesion ability to Caco-2 and IPEC-1 were detected in those strains. LP15-1 had excellent probiotic properties among the six strains, and could be used as a potential probiotic strain for further researches and application.

Key words： Lactobacillus plantarum; probiotic properties; acid tolerance; antibacterial ability; adhesion ability

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