食品与发酵工业 >

2022 , Vol. 48 >Issue 12: 46 - 51

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.028424

研究报告

熊果酸壳聚糖纳米颗粒的制备工艺优化及评价

  • 赵路洁 ,
  • 段续 ,
  • 曹伟伟 ,
  • 任广跃 ,
  • 刘盼盼 ,
  • 任星
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  • 1(河南科技大学 食品与生物工程学院,河南 洛阳,471023)
    2(粮食储藏安全河南省协同创新中心,河南 郑州,450001)
第一作者:硕士研究生(段续教授为通信作者,E-mail:duanxu_dx@163.com)

收稿日期: 2021-06-21

  修回日期: 2021-07-23

  网络出版日期: 2022-07-15

基金资助

国家自然科学基金(31671907);十三五国家重点研发计划(2017YFD0400901)

收起

Optimization and evaluation of preparation of ursolic acid loaded chitosan nanoparticles

  • ZHAO Lujie ,
  • DUAN Xu ,
  • CAO Weiwei ,
  • REN Guangyue ,
  • LIU Panpan ,
  • REN Xing
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  • 1(College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China)
    2(Collaborative Innovation Center of Grain Storage Security, Henan Province, Zhengzhou 450001, China)

Received date: 2021-06-21

  Revised date: 2021-07-23

  Online published: 2022-07-15

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摘要

采用离子凝胶法以三聚磷酸钠为交联剂制备负载熊果酸的壳聚糖纳米颗粒,通过单因素试验和正交试验,以熊果酸包埋率为考察指标,优化负载熊果酸的壳聚糖纳米颗粒的制备工艺,并评价最佳工艺下熊果酸纳米颗粒的粒径和体外溶出度。结果表明,熊果酸纳米颗粒最佳制备工艺参数为:壳聚糖溶液质量浓度为2.0 mg/mL,壳聚糖与熊果酸质量比为4∶1,壳聚糖与三聚磷酸钠质量比为4∶1,在此条件下的包埋率为79.52%。负载熊果酸的壳聚糖纳米颗粒的粒径在180 nm左右,且分布较窄。与未包埋的熊果酸相比,熊果酸纳米颗粒在模拟胃肠液中的溶出度分别增加至4.7倍和1.1倍。该研究为提高熊果酸的溶解性提供了借鉴。

关键词： 壳聚糖; 熊果酸; 纳米颗粒; 离子凝胶法; 正交试验

本文引用格式

赵路洁 , 段续 , 曹伟伟 , 任广跃 , 刘盼盼 , 任星 . 熊果酸壳聚糖纳米颗粒的制备工艺优化及评价[J]. 食品与发酵工业, 2022 , 48(12) : 46 -51 . DOI: 10.13995/j.cnki.11-1802/ts.028424

Abstract

The ursolic acid loaded chitosan nanoparticles were prepared by ionic gel method with sodium tripolyphosphate as the cross-linking agent. Based on the single factor and orthogonal experiment, the encapsulation efficiency was used as an evaluation indicator to optimize the preparation parameters of ursolic acid loaded chitosan nanoparticles. Moreover, the particle size and dissolution of ursolic acid nanoparticles under the optimized parameters were also determined. Results showed that the optimum conditions were as follows: the concentration of chitosan solution was 2.0 mg/mL; the mass ratio of chitosan to ursolic acid was 4∶1; the mass ratio of chitosan to sodium tripolyphosphate was 4∶1, and the encapsulation efficiency was 79.52%. The particle size of ursolic acid loaded chitosan nanoparticles was about 180 nm, and the distribution was narrow. Meanwhile, the solubility of ursolic acid could significantly increase. Compared with unencapsulated ursolic acid, the dissolution rate of ursolic acid nanoparticles in the simulated gastrointestinal fluid was increased by 4.7 and 1.1 times, respectively. This study provides reference for enhancing the solubility of ursolic acid.

Key words： chitosan; ursolic acid; nanoparticles; ionic gel method; orthogonal experimental

参考文献

[1] 张梦, 何曼, 孙强, 等.基于Hedgehog信号通路的熊果酸诱导结直肠癌SW480细胞凋亡的机制研究[J].中草药, 2021, 52(8):2 365-2 373.
ZHANG M, HE M, SUN Q, et al.Mechanism study on ursolic acid induced apoptosis of colorectal cancer SW480 cells based on Hedgehog signaling pathway[J].Chinese Traditional and Herbal Drugs, 2021, 52(8):2 365-2 373.
[2] 周茜, 韩雪, 韩晓梅, 等.响应面试验优化乌梅熊果酸提取工艺及其对大肠杆菌的抑制作用[J].食品科学, 2016, 37(8):67-73.
ZHOU Q, HAN X, HAN X M, et al.Optimization of ursolic acid extraction from fructus mume and evaluation of its antibacterial activity against Escherichia coli [J].Food Science, 2016, 37(8):67-73.
[3] 马浩然, 贾海莲, 张文龙, 等.熊果酸对酒精性肝损伤大鼠肠道菌群的影响[J].食品科学, 2018, 39(11):146-151.
MA H R, JIA H L, ZHANG W L, et al.Effect of ursolic acid on gut microbiota in rats with alcohol-induced liver injury[J].Food Science, 2018, 39(11):146-151.
[4] SONG S, GAO K, NIU R M, et al.Binding behavior, water solubility and in vitro cytotoxicity of inclusion complexes between ursolic acid and amino-appended β-cyclodextrins[J].Journal of Molecular Liquids, 2019, 296:111993.
[5] LÓPEZ-HORTAS L, PÉREZ-LARRÁN P, GONZÁLEZ-MUÑOZ M J, et al.Recent developments on the extraction and application of ursolic acid.A review[J].Food Research International, 2018,103:130-149.
[6] JESUS J A, LAGO J H G, LAURENTI M D, et al.Antimicrobial activity of oleanolic and ursolic acids:An update[J].Evidence-Based Complementary and Alternative Medicine, 2015, 2015:620472.
[7] SUN C X, XU C Q, MAO L K, et al.Preparation, characterization and stability of curcumin-loaded zein-shellac composite colloidal particles[J].Food Chemistry, 2017, 228:656-667.
[8] YAN J, GUAN Z Y, ZHU W F, et al.Preparation of puerarin chitosan oral nanoparticles by ionic gelation method and its related kinetics[J].Pharmaceutics, 2020, 12(3):216.
[9] 林洪斌, 郭训练, 刘茜,等.纳米壳聚糖粒子制备工艺优化及抗菌性研究[J].食品与发酵工业, 2020, 46(3):110-117.
LIN H B, GUO X L, LIU Q, et al.Preparation process optimization and antibacterial activity analysis of nano-chitosan particles[J].Food and Fermentation Industries, 2020, 46(3):110-117.
[10] 陈文彬, 严文静, 徐幸莲, 等.α-生育酚壳聚糖纳米粒的制备、表征及体外缓释抗氧化性能[J].食品科学, 2017, 38(22):216-223.
CHEN W B, YAN W J, XU X L, et al.Preparation, characterization and in vitro sustained antioxidant activity of α-tocopherol-loaded chitosan nanoparticles[J].Food Science, 2017, 38(22):216-223.
[11] HERNANDEZ-PATLAN D, SOLIS-CRUZ B, CANO-VEGA M A, et al.Development of chitosan and alginate nanocapsules to increase the solubility, permeability and stability of curcumin[J].Journal of Pharmaceutical Innovation, 2019, 14(2):132-140.
[12] WU J, WANG Y P, YANG H, et al.Preparation and biological activity studies of resveratrol loaded ionically cross-linked chitosan-TPP nanoparticles[J].Carbohydrate Polymers, 2017, 175:170-177.
[13] THANDAPANI G, P S P, P N S, et al.Size optimization and in vitro biocompatibility studies of chitosan nanoparticles-ScienceDirect[J].International Journal of Biological Macromolecules, 2017, 104:1 794-1 806.
[14] 谭啸, 邱婷婷, 李若男, 等.壳聚糖纳米粒子的制备和在食品抑菌中的研究进展[J].食品科学, 2020, 41(23):347-353.
TAN X, QIU T T, LI R N, et al.Recent progress in the preparation and application of chitosan nanoparticles as an antimicrobial in foods[J].Food Science, 2020, 41(23):347-353.
[15] YAN J, GUAN Z Y, ZHU W F, et al.Preparation of puerarin chitosan oral nanoparticles by ionic gelation method and its related kinetics[J].Pharmaceutics, 2020, 12(3):216-233.
[16] 龙门, 冯超, 李永佳, 等.缓释型茶树精油-壳聚糖微胶囊的制备、表征及体外释放规律[J].食品科学, 2019, 40(16):242-248.
LONG M, FENG C, LI Y J, et al.Preparation, characterization and in vitro release characteristics of sustained-release chitosan microcapsules containing tea tree oil[J].Food Science, 2019, 40(16):242-248.
[17] LI W J, PENG H L, NING F J, et al.Amphiphilic chitosan derivative-based core-shell micelles:Synthesis, characterisation and properties for sustained release of Vitamin D3[J].Food Chemistry, 2014, 152:307-315.
[18] 任海伟, 邢雪晔, 张湘越, 等.白酒糟制备高纯度微晶纤维素工艺优化及其结构表征[J].中国食品学报, 2020, 20(4):108-117.
REN H W, XING X Y, ZHANG X Y, et al.Preparation process optimization and structure characterization of microcrystalline cellulose from grains stillage[J].Journal of Chinese Institute of Food Science and Technologys, 2020, 20(4):108-117.
[19] 胡蒋宁, 熊超越, 李想, 等.超声制备中长链结构酯纳米乳液及其体外消化的研究[J].中国食品学报, 2019, 19(3):175-183.
HU J N, XIONG C Y, LI X, et al.Studies on the preparation of nano-emulsions with medium-and long-chain triacylglycerols and its digestion in vitro[J].Journal of Chinese Institute of Food Science and Technology, 2019, 19(3):175-183.
[20] 李淑坤, 汪晶, 仝萌, 等.姜黄色素-共聚维酮共研磨粉体性质及溶出度研究[J].中草药, 2020, 51(23):5 949-5 955.
LI S K, WANG J, TONG M, et al.Properties and dissolution of curcumin-PVP co-grinding powder[J].Chinese Traditional and Herbal Drugs, 2020, 51(23):5 949-5 955.
[21] LIU Z Z, YANG L.Antisolvent precipitation for the preparation of high polymeric procyanidin nanoparticles under ultrasonication and evaluation of their antioxidant activity in vitro[J].Ultrasonics Sonochemistry, 2018, 43:208-218.
[22] 张睿. 气-液包埋法制备肉桂油-β-环糊精微胶囊的研究[D].广州:广东工业大学, 2017.
ZHANG R.Study on preparation of cinnamon oil-β-cyclodextrin microcapsules by gas-liquid embedding method[D].Guangzhou:Guangdong University of Technology, 2017.
[23] 滑海涛, 李敏, 翟晓曼, 等.反应时间、芯壁比及表面活性剂用量对阿维菌素微囊制备的影响[J].农药学学报, 2010, 12(1):54-60.
HUA H T, LI M, ZHAI X M, et al.Studies on the influence of polymerization time ratio of core material to wall material and percentage of SDS on the preparation of abamectin capsule suspensions[J].Chinese Journal of Pesticide Science, 2010, 12(1):54-60.
[24] 张依诺, 吴珊, 刘峥, 等.靶向羧甲基壳聚糖-熊果酸纳米药物载体的制备及表征[J].精细与专用化学品, 2019, 27(2):38-41.
ZHANG Y N, WU S, LIU Z, et al.Preparation and characterization of targeted carboxymethyl chitosan-ursolic acid nano-drug carriers[J].Fine and Specialty Chemicals, 2019, 27(2):38-41.
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