食品与发酵工业 >

2022 , Vol. 48 >Issue 12: 145 - 151

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.028209

研究报告

超高压和发芽对大豆中主要营养成分生物利用率的影响

  • 朱吟非 ,
  • 谭美 ,
  • 吴新怡 ,
  • 王超 ,
  • 段翰英
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  • (暨南大学 食品科学与工程系,广东 广州,510632)
第一作者:朱吟非(本科生)和谭美(硕士研究生)为共同第一作者(段翰英讲师为通信作者,E-mail:tduhy@jnu.edu.cn)

收稿日期: 2020-06-04

  修回日期: 2021-07-15

  网络出版日期: 2022-07-15

基金资助

广东省重点领域研发计划(2020B020225003);广东省自然科学基金(2021A1515010606)

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Effects of high hydrostatic pressure and germination on nutrient bioavailability in soybeans

  • ZHU Yinfei ,
  • TAN Mei ,
  • WU Xinyi ,
  • WANG Chao ,
  • DUAN Hanying
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  • (Food Science and Technology Department, Jinan University, Guangzhou 510632, China)

Received date: 2020-06-04

  Revised date: 2021-07-15

  Online published: 2022-07-15

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摘要

为探讨大豆浸泡后经发芽、超高压(high hydrostatic pressure,HHP)处理对其主要营养成分生物利用率的影响,利用体外消化测定发芽或HHP处理(300、450、600 MPa处理5、10 min)后的大豆的游离氨基酸(free amino acids,FAAs)含量、淀粉体外消化量(in vitro starch digestibility,IVSD)及铁(Fe)、锌(Zn)、钙(Ca)的消化率。同时,测定植酸、单宁这2种抗营养因子含量。结果表明,与未处理组相比,较高压力的HHP(450、600 MPa)处理可提高各FAAs含量和蛋白质体外消化率,但未影响氨基酸种类分布。发芽提高了大豆的IVSD至2.42倍,而600 MPa/10 min处理则降低了38%。Fe消化率经发芽、450 MPa/10 min和600 MPa/10 min处理后分别显著提高至2.07、2.06和1.63倍,经600 MPa/5 min处理后则提高了12%(P<0.05)。发芽和HHP均能显著提高Zn的消化率(P<0.05)。发芽未对Ca的消化率产生影响,而HPP处理后Ca的消化率升高为未处理组的1.91倍。发芽、300 MPa处理可显著降低植酸含量(P<0.05),450 MPa/10 min处理显著降低单宁含量(P<0.05)。该研究为开发新的大豆非热加工工艺及高附加值大豆产品提供了理论依据。

关键词： 超高压; 发芽; 生物利用率; 抗营养因子; 大豆

本文引用格式

朱吟非 , 谭美 , 吴新怡 , 王超 , 段翰英 . 超高压和发芽对大豆中主要营养成分生物利用率的影响[J]. 食品与发酵工业, 2022 , 48(12) : 145 -151 . DOI: 10.13995/j.cnki.11-1802/ts.028209

Abstract

Effects of germination and high hydrostatic pressure (HHP) treatment on the bioavailability of main nutrients in soybeans after soaking were explored. After germination and HHP treatment (300, 450, 600 MPa for 5, 10 min), free amino acids (FAAs) content, in vitro starch digestibility (IVSD), digestibility of minerals (Fe, Zn, Ca) were determined by in vitro digestion. The contents of phytic acid and tannin were also determined. The results showed that compared with the untreated group, the FAAs content and the in vitro protein digestibility (IVPD) increased under high pressure (450 and 600 MPa), but the distribution of amino acids was not affected. Germination increased the IVSD of soybean by 2.42 times, while the treatment of 600 MPa/10 min decreased by 38%. The Fe digestibility was significantly increased to 2.07, 2.06 and 1.63 times after germination, 450 MPa/10 min and 600 MPa/10 min treatment, respectively, and increased by 12% after 600 MPa/5min treatment(P<0.05). Germination and HHP significantly increased Zn digestibility (P<0.05). Germination did not affect the digestibility of Ca, while HPP treatment could reach 1.91 times that of the untreated group. Germination and 300 MPa decreased the phytic acid content and 450 MPa/10 min decreased the tannin content (P<0.05). This study provides a theoretical basis for the development of new soybean non-thermal processing technology and high value-added soybean products.

Key words： high hydrostatic pressure; germination; nutrient bioavailability; anti-nutritional factors; soybeans

参考文献

[1] 王新坤. 钙处理改善大豆芽菜营养与功能品质的机制[D].南京:南京农业大学,2016.
WANG X K.The improved nutritional and functional quality of soybean sprouts by calcium[D].Nanjing:Nanjing Agricultural University,2016.
[2] 吕远平,姚开,贾冬英.饲料中植物单宁的抗营养性及其生物降解[J].中国畜牧杂志,2003,39(2):42-43.
LYU Y P, YAO K, JIA D Y.Antinutrition and biodegradation of vegetable tannins in forage[J].Chinese Journal of Animal Science,2003,39(2):42-43.
[3] MUBARAK A E.Nutritional composition and antinutritional factors of mung bean seeds (Phaseolus aureus) as affected by some home traditional processes[J].Food Chemistry,2004,89(4):489-495.
[4] 姜宇婷. 绿豆发芽过程中组分及营养变化研究进展[J].现代农业科技,2020(14):209;214.
JIANG Y T.Research progress on composition and nutrient changes of mung bean during germination[J].Modern Agricultural Science and Technology,2020(14):209;214.
[5] VAGADIA B H, VANGA S K, SINGH A, et al.Comparison of conventional and microwave treatment on soymilk for inactivation of trypsin inhibitors and in vitro protein digestibility[J].Foods,2018,7(1):7010006.
[6] HERNÁNDEZ-CARRIÓN M, TÁRREGA A, HERNANDO I, et al.High hydrostatic pressure treatment provides persimmon good characteristics to formulate milk-based beverages with enhanced functionality[J].Food & Function,2014,5(6):1 250-1 260.
[7] 李仁杰, 廖小军, 胡小松, 等.超高压对蛋白质的影响[J].高压物理学报,2014,28(4):498-506.
LI R J, LIAO X J, HU X S, et al.Effects of high hydrostatic pressure on proteins[J].Chinese Journal of High Pressure Physics,2014,28(4):498-506.
[8] 赵贵川. 超高压处理对米渣蛋白水解物抗氧化活性的影响[D].长沙:中南林业科技大学,2016.
ZHAO G C.The research of UHP treatment on rice residue protein hydrolyzate antioxidant activity[D].Changsha:Central South University of Forestry and Technology,2016.
[9] LINSBERGER-MARTIN G, WEIGLHOFER K, THAO P T P, et al.High hydrostatic pressure influences antinutritional factors and in vitro protein digestibility of split peas and whole white beans[J].LWT - Food Science and Technology,2013,51(1):331-336.
[10] 郭蔚波, 赵燕, 徐明生, 等.不同处理方式下蛋白质结构变化与体外消化性关系研究进展[J].食品科学,2019,40(1):327-333.
GUO W B, ZHAO Y, XU M S, et al.Recent progress in understanding the relationship between protein structure change and in vitro digestibility after different treatments[J].Food Science,2019,40(1):327-333.
[11] GUERRERO-BELTRÁN J A, ESTRADA-GIRÓN Y, SWANSON B G, et al.Pressure and temperature combination for inactivation of soymilk trypsin inhibitors[J].Food Chemistry,2009,116(3):676-679.
[12] 于勇, 潘芳, 苏光明, 等.超高压技术在粮食产品加工中的应用[J].农业机械学报,2015,46(10):247-256;297.
YU Y, PAN F, SU G M, et al.Application of ultra high pressure on crop produces processing[J].Transactions of the Chinese Society for Agricultural Machinery,2015,46(10):247-256;297.
[13] LI W H, BAI Y F, MOUSAA S A S, et al.Effect of high hydrostatic pressure on physicochemical and structural properties of rice starch[J].Food and Bioprocess Technology,2012,5(6):1-9.
[14] RAMÍREZ-CÁRDENAS L, LEONEL A J, COSTA N M B.Efeito do processamento doméstico sobre o teor de nutrientes e de fatores antinutricionais de diferentes cultivares de feijão comum[J].Ciência e Tecnologia de Alimentos, 2008,28(1):200-213.
[15] SANTOS M C, NUNES C, FERREIRA A S, et al.Comparison of high pressure treatment with conventional red wine aging processes:Impact on phenolic composition[J].Food Research International, 2018, 116.DOI:10.1016/j.foodres.2018.08.018
[16] WANG C, DUAN H Y, TENG J W.Assessment of microwave cooking on the bioaccessibility of cadmium from various food matrices using an in vitro digestion model[J].Biological Trace Element Research, 2014, 160(2):276-284.
[17] SINGH U, JAMBUNATHAN R.Changes in starch, oligosaccharides and soluble sugars in developing pod wall and seed of chickpea[J].Pergamon,1982,21(2):297-299.
[18] 杨宁, 王伟明, 姚琳, 等.3,5-二硝基水杨酸法测定发酵型果露酒中总糖含量[J].中国酿造,2018,37(1):181-184.
YANG N, WANG W M, YAO L, et al.Determination of total sugar content in fermented fruit wine by 3, 5-dinitrosalicylic acid method[J].China Brewing,2018,37(1):181-184.
[19] MCKIE V, MCCLEARY B V.A novel and rapid colorimetric method for measuring total phosphorus and phytic acid in foods and animal feeds[J].Journal of AOAC International,2016,99(3):738-743.
[20] LEE H, HA M J, SHAHBAZ H M, et al.High hydrostatic pressure treatment for manufacturing of red bean powder:A comparison with the thermal treatment[J].Journal of Food Engineering,2018,238:141-147.
[21] VENY U, KIRAN B.Effect of germination periods and hydrothermal treatments on in vitro protein and starch digestibility of germinated legumes[J].Journal of Food Science and Ttechnology,2012,49(2):184-191.
[22] XIA Q, WANG L P, XU C C, et al.Effects of germination and high hydrostatic pressure processing on mineral elements, amino acids and antioxidants in vitro bioaccessibility, as well as starch digestibility in brown rice (Oryza sativa L.)[J].Food Chemistry,2017,214:533-542.
[23] ZHONG Y, WANG Z Y, ZHAO Y Y.Impact of radio frequency, microwaving, and high hydrostatic pressure at elevated temperature on the nutritional and antinutritional components in black soybeans[J].Journal of Food Science,2015,80(12):2 732-2 739.
[24] MESSENS W, VAN CAMP J, HUYGHEBAERT A.The use of high pressure to modify the functionality of food proteins[J].Trends in Food Science & Technology,1997,8(4):107-112.
[25] 卞科,李雅楠,关二旗.甘薯抗性淀粉的制备工艺及应用研究进展[J].河南工业大学学报(自然科学版),2017,38(2):122-128.
BIAN K, LI Y N, GUAN E Q.Preparation and application of sweet potato resistant starches[J].Journal of Henan University of Technology(Natural Science Edition),2017,38(2):122-128.
[26] LIU H, FAN H H, CAO R, et al.Physicochemical properties and in vitro digestibility of sorghum starch altered by high hydrostatic pressure[J].International Journal of Biological Macromolecules, 2016, 92:753-760.
[27] DENG Y, JIN Y F, LUO Y L, et al.Impact of continuous or cycle high hydrostatic pressure on the ultrastructure and digestibility of rice starch granules[J].Journal of Cereal Science, 2014, 60(2):302-310.
[28] SINGH J, DARTOIS A, KAUR L.Starch digestibility in food matrix:A review[J].Trends in Food Science & Technology,2009,21(4):168-180.
[29] O′DELL B L.Mineral interactions relevant to nutrient requirements[J].Journal of Nutrition,1989,119(12):1 832-1 838.
[30] 任顺成, 万毅, 李丹.超高压对麦麸及其植酸含量变化的影响[J].食品研究与开发,2019,40(7):29-33.
REN S C, WAN Y, LI D.Effect of ultra-high pressure on wheat bran and its phytic acid content[J].Food Research and Development,2019,40(7):29-33.
[31] MOHAMMED M A, MOHAMED E A, YAGOUB A E A, et al.Effect of processing methods on alkaloids, phytate, phenolics, antioxidants activity and minerals of newly developed lupin (Lupinus albus L.) cultivar[J].Journal of Food Processing and Preservation,2017,41(1):e12960.1-e12960.9.
[32] BELMIRO R H, TRIBST A A L, CRISTIANINI M.Effects of high pressure processing on common beans (Phaseolus vulgaris L.):Cotyledon structure, starch characteristics, and phytates and tannins contents[J].Starch-Stärke,2020,72(3-4).DOI:10.1002/star.201900212.
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