食品与发酵工业 >

2022 , Vol. 48 >Issue 15: 124 - 130

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.029482

研究报告

添加氮源和金属离子提高深黄伞形霉利用麸皮水解液积累油脂的研究

  • 冯爱博 ,
  • 周化岚 ,
  • GULTOM Sarman Oktovianus ,
  • 张建国
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  • 1(上海理工大学 健康科学与工程学院,食品科学与工程研究所,上海,200093)
    2(Department of Agricultural and Biosystems Engineering,Papua University Manokwari,Papua Barat,Indonesia)
第一作者:博士研究生(张建国教授为通信作者,E-mail:jgzhang@usst.edu.cn)

收稿日期: 2021-09-29

  修回日期: 2021-11-21

  网络出版日期: 2022-09-02

基金资助

上海市国际科技合作基金项目(19230742900)

收起

Enhancing of lipid accumulation of Umbelopsis isabellina by addition of ammonium and metal ion in the wheat bran hydrolysate

  • FENG Aibo ,
  • ZHOU Hualan ,
  • GULTOM Sarman Oktovianus ,
  • ZHANG Jianguo
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  • 1(Institute of Food Science and Engineering, School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China)
    2(Department of Agricultural and Biosystems Engineering, Papua University Manokwari, Papua Barat, Indonesia)

Received date: 2021-09-29

  Revised date: 2021-11-21

  Online published: 2022-09-02

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摘要

深黄伞形霉是积累油脂的重要微生物,以深黄伞形霉利用麸皮水解液生成油脂有利于麸皮的综合利用。基于前期研究,在麸皮水解液中添加氮源、金属离子提高了油脂产量和麸皮水解液中糖分的利用。当乙酸铵添加至氮元素为1.5 g/L时,深黄伞形霉的生物量达到最大值25.8 g/L。乙酸铵添加至氮元素为1.0 g/L时,油脂产量提高11%。麸皮水解液中添加Mg2+、Ca2+和Mn2+促进深黄伞形霉的代谢和脂肪合成。当添加0.5 g/L CaCl2、1.0 g/L MgSO4、8 μg/L MnSO4时油脂产量分别提高脂肪酸分别达到13.8、10.26、12.91 g/L,分别提高了39.8%、65.8%、30.8%。研究结果为麸皮的综合利用提供了参考。

关键词： 深黄伞形霉; 麸皮水解液; 油脂积累; 氮源; 金属离子

本文引用格式

冯爱博 , 周化岚 , GULTOM Sarman Oktovianus , 张建国 . 添加氮源和金属离子提高深黄伞形霉利用麸皮水解液积累油脂的研究[J]. 食品与发酵工业, 2022 , 48(15) : 124 -130 . DOI: 10.13995/j.cnki.11-1802/ts.029482

Abstract

Umbelopsis (Mortierella) isabellina is an important oleaginous microbial species. Lipid production by U. isabellina using wheat bran hydrolysate benefits the utilization of wheat bran. Based on our previous study, this study investigated the influences of nitrogen sources and metal ions on cell growth and lipid production of U. isabellina, and carbon source utilization. The results showed that the maximum of biomass was obtained at 1.5 g/L nitrogen using ammonium acetate, and 11% increment of oil production at 1.0 g/L nitrogen using ammonium acetate. The addition of Mg2+, Ca2+ and Mn2+ in wheat bran hydrolysate promoted carbon metabolism and lipid synthesis of U. isabellina. The lipid yield reached 13.8, 10.26, 12.91 g/L with 39.8%, 65.80%, 30.8% increment respectively at 0.5 g/L CaCl2, 1.0 g/L MgSO4, 8 μg/L MnSO4 addition. These results provided a base for application of wheat bran in food industry.

Key words： Umbelopsis isabellina (Mortierella isabellina); wheat bran hydrolysate, lipid accumulation; nitrogen source; metal ion

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