旨在生产高活菌数、高稳定性的枯草芽孢杆菌(Bacillus subtilis)BS08微生态活菌制剂。采用喷雾干燥技术,通过单因素实验从蛋白质类、碳水化合物类、亲水胶体类保护剂中筛选较优壁材进行复配联用,并以菌粉活菌数和稳定性为指标,研究抗热保护剂的包埋顺序对枯草芽孢杆菌保护效果的影响;最后,采用响应面优化技术对复合抗热保护剂配方进行了优化。结果表明,以明胶-阿拉伯胶-海藻糖的从内而外的包埋顺序复配成保护剂,能有效保护微胶囊中的枯草芽孢杆菌,且通过稳定性评估,产品在4 ℃环境中贮藏1年,预计活菌数可保持在1014以上,数量级不变。经过响应面的优化,当明胶质量分数为26%,阿拉伯胶质量分数为24%,海藻糖质量分数为24%,枯草芽孢杆菌微生态制剂的活菌数最高可达5.98×1014CFU/g。
The purpose of this study is to produce the probiotics of Bacillus subtilis BS08 with high viable count and high stability.Spray drying technology was used to select better wall materials from protein, carbohydrate and hydrocolloid protectant by single factor experiment.And, the number of viable bacteria and stability of bacterial powder was used as indicators to study the effect of the embedding order of anti-thermal protectant on the protective effect of BS08. Finally, the response surface methodology was applied to optimize the formulation of compound anti-thermal protectant.The results showed that the anti-thermal protectant composed of gelatin, arabic gum and trehalose from the inside to the outside could effectively protect BS08 in microcapsules.Through stability evaluation, it is estimated that the viable count of the product were >1014 CFU/g if storage for 1 year at 4 ℃, with the same order of magnitude.After response surface optimization, when the mass fraction of gelatin, Arabic gum and trehalose is 26%, 24%, and 24%, the maximum viable count of BS08 is 5.98×1014 CFU/g.
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