黄水芽胞杆菌Bacillus aquiflavi 3H-10分离自我国四川省宜宾地区传统发酵浓香型白酒黄水样品,具有产生蛋白酶和多种风味物质的能力。对黄水芽胞杆菌3H-10进行高通量基因组测序,并开展耐药基因、毒力基因和致病性分析,以及药物敏感性检测、小鼠毒力实验,以综合评价黄水芽胞杆菌3H-10的安全性。结果显示,黄水芽胞杆菌3H-10基因组为一条环状的DNA,大小3 806 094 bp,G+C含量为35.66%(摩尔百分含量),基因组中预测到4 031个基因、81个tRNA基因、27个rRNA基因、16个sRNA基因;3H-10基因组中未检测到潜在耐药基因,且对常见的9大类、10种抗生素表现为敏感,判断菌株无横向转移耐药性的风险;3H-10基因组中未检测到潜在毒力相关基因,受试小鼠不产生毒性反应或死亡,判断菌株无毒力或人体致病性。黄水芽胞杆菌3H-10在耐药性、毒力与致病性方面无安全性风险。
Bacillus aquiflavi 3H-10 was isolated from yellow water of strong-flavor Chinese Baijiu in Yibin, Sichuan province, China, and it had significant ability in producing protease and various flavor substances. High-throughput genome sequencing of strain 3H-10 was carried out and antimicrobial resistance genes, virulence factors genes, pathogenicity, antimicrobial susceptibility testing and virulence test in mice were analyzed to comprehensively evaluate the safety of the strain. The result showed that the genome of strain 3H-10 was a circular DNA with a size of 3 806 094 bp and the G+C content was 35.66 mol%. A total of 4 031 genes were predicted along with 81 tRNA, 27 rRNA and 16 sRNA. No potential antimicrobial resistance genes were detected in the genome of 3H-10 and the strain was sensitive to 10 common antibiotics affiliated with nine classes, indicating no risks of horizontally transferring antimicrobial resistance. No potential virulence genes were detected in the genome of strain 3H-10, and the virulence test demonstrated no toxic reaction or death in mice, implying that the strain 3H-10 was non-virulent or non-human pathogenic. It was concluded that strain B. aquiflavi 3H-10 possesses no safety risks in the aspect of antimicrobial resistance, virulence or pathogenicity.
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