该研究旨在探究植物乳杆菌(Lactobacillus plantarum)P-8对小鼠肠道菌群及抗氧化抗炎能力的影响,为植物乳杆菌P-8相关益生菌制剂的开发利用提供依据。将Balb/c雄性鼠52只随机均分低、中、高剂量组和空白组,分别灌胃等体积的生理盐水和不同剂量的植物乳杆菌P-8溶液,连续30 d。测定小鼠的体质量、摄食饮水变化,并对粪便样品进行活菌计数及16S rDNA高通量测序,处死小鼠后计算脏器系数,测定小肠、肝脏组织中总超氧化物歧化酶(total superoxide dismutase, T-SOD)、丙二醛、谷胱甘肽和谷胱甘肽过氧化物酶(glutathione peroxidase, GSH-Px)的水平及血清中炎症反应指标(IL-1β、IL-6)。结果表明,与空白组相比,植物乳杆菌P-8溶液灌胃对小鼠的摄食饮水、体质量和脏器系数无显著差异(P>0.05);高剂量组小肠和肝脏组织中谷胱甘肽、T-SOD和GSH-Px的含量显著升高(P<0.05),丙二醛含量显著降低(P<0.05);中剂量组肝脏组织中GSH-Px的含量显著升高(P<0.05)。与空白组相比,高剂量组血清中IL-1β、IL-6显著降低(P<0.05)。灌胃30 d后,空白组、低剂量和中剂量组各类微生物数量与灌胃前相比无显著差异(P>0.05),而中高剂量组乳杆菌、双歧杆菌的数量显著增加(P<0.05),16S rDNA高通量测序结果表明,与空白组相比,低剂量组乳杆菌属的丰度显著增加(P<0.05),中高剂量组乳杆菌属的丰度极显著上升(P<0.01),高剂量组Odoribacter的丰度显著增加(P<0.05),Muribaculum、芽孢杆菌属的丰度显著降低(P<0.05)。综上,植物乳杆菌P-8能够提高小鼠抗氧化能力,降低血清炎症水平,调节肠道菌群组成,在益生菌制剂的开发中具有良好的应用潜力。
To test the effects of Lactobacillus plantarum P-8 on the intestinal flora, antioxidant and anti-inflammatory capacity of mice for providing a basis for the development and utilization of a probiotic preparation of L. plantarum P-8, Fifty-two BALB/c male mice were randomly divided into blank control, low, medium and high-dose groups and administered with normal saline and different doses of probiotic preparation of L. plantarum P-8 for 30 d. The results showed that, compared with the control, there was no significant difference on the food intake, body weight and organ coefficient in each dose group(P>0.05), the levels of GSH, T-SOD and GSH-Px in the small intestine and liver tissues significantly increased (P<0.05) and the levels of MDA significantly decreased in the high-dose group (P<0.05) and the levels of GSH-Px significantly increased in the medium-dose group (P<0.05). The levels of IL-1β and IL-6 significantly decreased in the high-dose group (P<0.05). After administration, there was no significant difference in the number of microorganisms in blank control, low and medium-dose groups (P>0.05), the number of Lactobacillus and Bifidobacterium significantly increased in the high-dose group (P>0.05). 16S rDNA high-throughput sequencing results showed that, compared with the control, the abundance of Lactobacillus significantly increased in the low-dose group (P<0.05) and extremely increased in the medium and high-dose group (P<0.05), the abundance of Odoribacter significantly increased (P<0.05) and that of Muribaculum and Bacillus decreased in the high-dose group significantly (P<0.05). In summary, L. plantarum P-8 can improve the antioxidant capacity, reduce serum inflammation level, regulate the composition of intestinal flora and has a great application potential in the development of probiotic preparation.
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