含油率高是影响餐厨垃圾处理和利用的难题之一。研究对不同来源样品进行富集驯化、油脂降解平板筛选,获得1株油脂高效降解菌JY34,经16S rDNA鉴定为酯香微杆菌(Microbacterium esteraromaticum)。探索了该菌在不同温度、酸碱度、接种量以及含油量条件下的油脂降解率,并评估其脂肪酶活力大小和在餐厨垃圾含油废水中实际油脂降解率,结果表明JY34在30 ℃、pH 7.0、接种量2%、含油量1%条件下发酵3 d后,大豆油降解率高达98.78%。JY34发酵2 d后的粗酶液在30~50 ℃下的脂肪酶活力随反应温度的升高不断增加,50 ℃时达159.5 U/L。将JY34接入餐厨垃圾含油废水中,发酵3、6 d后的油脂降解率分别达到47.49%和72.92%,是对照组的4.1倍和1.63倍。综上,菌株JY34具有良好的中温适应性以及油脂降解能力,具有广阔的应用前景。
易蒲红
,
李芩萍
,
赵彩虹
,
丁晓艳
,
王宁
,
朱廷恒
,
李季
. 餐厨垃圾中油脂高效降解菌酯香微杆菌(Microbacterium esteraromaticum)的分离及其应用[J]. 食品与发酵工业, 2022
, 48(17)
: 143
-149
.
DOI: 10.13995/j.cnki.11-1802/ts.030250
The high lipid content of kitchen waste has become one of the problems affecting its treatment and utilization. Through enrichment of samples from different environmental sources and screening on lipid degradation plates, a highly efficient lipid degradation bacterium strain JY34 was obtained and identified as Microbacterium esteraromaticum by 16S rDNA analysis. The lipid degradation rate of JY34 under different temperature, pH, inoculation amount and lipid content conditions were explored, and its lipase activity and lipid degradation rate in the wastewater of kitchen waste were evaluated. The results showed that the soybean oil degradation rate of JY34 was 98.78% at 30℃, pH 7.0, the inoculation amount of 2% and lipid content of 1% after fermentation for 3 d. The lipase activity in the crude enzyme solution of JY34 increased with temperature rising among in the range of 30-50℃, reaching 159.5 U/L at 50℃ after fermentation for 2 d. After JY34 strain was fermented in the kitchen waste lipid-containing wastewater for 3 d or 6 d, the lipid degradation rate reached 47.49% or 72.92%, respectively, which were 4.1 times and 1.63 times than that of control. In summary, strain JY34 has good adaptability to mesophilic condition and lipid degradation ability, so it will have a broad application prospect in the treatment of oily wastewater from kitchen waste.
[1] 任连海, 王攀, 贺艳坤.含油量对餐厨垃圾好氧堆肥的影响[J].建设科技, 2013(8):50-53.
REN L H, WANG P, HE Y K.Effect of oil content on aerobic composting of kitchen waste[J].Construction Science and Technology, 2013(8):50-53.
[2] GAO L L, LU Y C, ZHANG J L, et al.Biotreatment of restaurant wastewater with an oily high concentration by newly isolated bacteria from oily sludge[J].World Journal of Microbiology & Biotechnology, 2019, 35(11):179.
[3] WITHARANA A, MANATUNGE J, RATNAYAKE N, et al.Rapid degradation of FOG discharged from food industry wastewater by lipolytic fungi as a bioaugmentation application[J].Environmental Technology, 2018, 39(16):2 062-2 072.
[4] SUGIMORI D.Edible oil-degradation by using yeast coculture of Rhodotorula pacifica ST3411 and Cryptococcus laurentii ST3412[J].Applied Microbiology and Biotechnology, 2009, 82(2):351-357.
[5] 卢永昌, 张继龙, 张建栋, 等.餐饮高浓度含油废水降解菌筛选及降解特性研究[J].工业水处理, 2019, 39(12):64-68;100.
LU Y C, ZHANG J L, ZHANG J D, et al.Bacterial screening for degradation of high concentration oily wastewater in catering industry and its degradation characteristics[J].Industrial Water Treatment, 2019, 39(12):64-68;100.
[6] REN J, FAN B, HUHETAOLI, et al.Biodegradation of waste cooking oils by Klebsiella quasivariicola IUMR-B53 and characteristics of its oil-degrading enzyme[J].Waste and Biomass Valorization, 2021, 12(3):1 243-1 252.
[7] 徐昆鹏. 含油废水处理方法的研究现状及展望[J].化工管理, 2021(24):21-22.
XU K P.Research status and prospect of oily wastewater treatment methods[J].Chemical Enterprise Management, 2021(24):21-22.
[8] 王长青, 张西华, 宁朋歌,等.含油废水处理工艺研究进展及展望[J].化工进展, 2021, 40(1):451-462.
WANG C Q, ZHANG X H, NING P G, et al.Research advances and perspective on treatment processes for oily wastewater[J].Chemical Industry and Engineering Progress, 2021, 40(1):451-462.
[9] GAUR N, NARASIMHULU K, Y P.Recent advances in the bio-remediation of persistent organic pollutants and its effect on environment[J].Journal of Cleaner Production, 2018, 198:1 602-1 631.
[10] BECKER P, KÖSTER D, POPOV M N, et al.The biodegradation of olive oil and the treatment of lipid-rich wool scouring wastewater under aerobic thermophilic conditions[J].Water Research, 1999, 33(3):653-660.
[11] 沈叔平, 汪小梅.废水中动植物油脂的紫外分光光度测定法[J].中国环境监测, 1994, 10(3):4-7.
SHEN S P, WANG X M.Determination of animal and vegetable fats and oils in wastewater by ultraviolet spectrophotometry[J].Environmental Monitoring in China, 1994, 10(3):4-7.
[12] 梁秋艳. 低温脂肪酶产生菌株的筛选、发酵条件优化及其酶学性质研究[D].石河子:石河子大学, 2014.
LIANG Q Y.Study on the screening, optimization of its fermentation conditions and enzymatic properties of a cold-adapted lipase-producing strain[D].Shihezi:Shihezi University, 2014.
[13] 王森, 张卡, 王泳浩, 等.一株油脂降解菌的筛选鉴定及降解效果分析[J].生物技术进展, 2021, 11(1):99-104.
WANG S, ZHANG K, WANG Y H, et al.Screening and identification of an oil degrading bacterium and analysis of its explanation effect[J].Current Biotechnology, 2021, 11(1):99-104.
[14] LEMUS G R, LAU A K.Biodegradation of lipidic compounds in synthetic food wastes during composting[J].Canadian Biosystems Engineering, 2002, 44(6):33-39.
[15] ONWOSI C O, IGBOKWE V C, ODIMBA J N, et al.Composting technology in waste stabilization:On the methods, challenges and future prospects[J].Journal of Environmental Management, 2017, 190:140-157.
[16] 刘小玉, 付登强.产脂肪酶蜡状芽孢杆菌菌株分离和鉴定[J].中国热带农业, 2021(6):68-73.
LIU X Y, FU D Q.Isolation and identification of a lipase producing strain of Bacillus cereus[J].China Tropical Agriculture, 2021(6):68-73.
[17] 辛国芹. 一株产脂肪酶菌株的分离筛选及鉴定[J].广东饲料, 2021, 30(8):27-29.
XIN G Q.Isolation, screening and identification of a lipase-producing strain[J].Guangdong Feed, 2021, 30(8):27-29.
[18] 程爽, 包朋鑫, 徐玉晨, 等.一株脂肪酶产生菌的筛选鉴定及其发酵条件优化[J].中国食品添加剂, 2021, 32(11):73-80.
CHENG S, BAO P X, XU Y C, et al.Isolation, identification of a lipase-producing bacterium and its optimized fermentation conditions[J].China Food Additives, 2021, 32(11):73-80.
