To investigate the promoting effects of collagen peptides (CP) and rice peptides (RP) on skin moisturizing, whitening and anti-oxidation. CP, RP and their complexes on skin care were investigated in vitro, with 5% glycerol as positive control, the water retention of the samples was investigated by weight loss method. Total antioxidant capacity was used to evaluate the antioxidant capacity of samples. The whitening activity of samples was evaluated by tyrosinase inhibition method. Based on the results of in vitro experiments, the proportion of cells was designed. The changes of superoxide dismutase (SOD) activity and reactive oxygen species (ROS) level in mouse melanoma cells (B16) were investigated to reflect the antioxidant capacity of the samples. The activity of tyrosinase in B16 cells was investigated to reflect the whitening ability of B16 cells. The results showed that both CP and RP showed good moisturizing activity in vitro, meanwhile, CP and its complex with RP in a certain proportion were superior to 5% glycerol under the same conditions. Both peptides had antioxidant function in vitro (ORAC method), and could inhibit tyrosinase activity, and synergistic synergistic effect was obtained when they were combined. The complex could scavenge ROS in B16 cells, improve the activity of SOD in B16 cells, and significantly reduce the activity of tyrosinase in B16 cells. The optimal complex ratio was CP:RP=2:1. Moreover, CP showed better antioxidant and moisturizing effects, while RP had better inhibitory effects on tyrosinase activity. The combined raw material could have synergistic effect in promoting moisture retention, anti-oxidation and inhibiting tyrosinase compared to each of them. The combination of collagen peptides and rice peptides in the ratio of 2:1 showed a stronger moisturizing effect than 5% glycerol in vitro, and it also showed a stronger tyrosinase inhibitory activity. In the cell experiment of mouse melanoma cell B16, it could clear the intracellular ROS, improve the activity of intracellular SOD, and significantly reduce the activity of tyrosinase. Collagen peptides and rice peptides at the ratio of 2:1 may play a positive role in skin health improvement, such as moisturizing, whitening and anti-oxidation.
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