研究报告

丁香精油对杧果采后炭疽病及品质的影响

  • 李俊国 ,
  • 胡美姣 ,
  • 弓德强 ,
  • 母军霞 ,
  • 王伟博 ,
  • 高兆银 ,
  • 李敏 ,
  • 谷会 ,
  • 陈千付 ,
  • 雷新涛
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  • 1(海南大学 园艺学院,海南 海口,570228)
    2(中国热带农业科学院热带作物品种资源研究所,海南 海口,571101)
    3(中国热带农业科学院环境与植物保护研究所,海南 海口,571101)
    4(海南省热带园艺产品采后生理与保鲜重点实验室,海南 海口,571101)
第一作者:硕士研究生(雷新涛研究员为通信作者,E-mail:xtlei@263.net)

收稿日期: 2021-08-24

  修回日期: 2021-10-13

  网络出版日期: 2022-10-17

基金资助

国家重点研发计划(2019YFD1001103);国家重点研发计划(2020YFD1000600);基本科研业务费项目(1630042017026)

Effects of clove essential oil on postharvest anthracnose and quality of mango

  • LI Junguo ,
  • HU Meijiao ,
  • GONG Deqiang ,
  • MU Junxia ,
  • WANG Weibo ,
  • GAO Zhaoyin ,
  • LI Min ,
  • GU Hui ,
  • CHEN Qianfu ,
  • LEI Xintao
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  • 1(Institute of Horticulture, Hainan University, Haikou 570228, China) 2 (Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China)3 (Environment and Plant Protection Institute, China Academy of Tropical Agricultural Sciences, Haikou 571101, China)
    4(Key Laboratory of Hainan Province for Postharvest Physiology and Technology of Tropical Horticultural Products, Haikou 571101, China)

Received date: 2021-08-24

  Revised date: 2021-10-13

  Online published: 2022-10-17

摘要

为探究植物精油对杧果采后炭疽病菌的抑菌效果以及植物精油处理后对贵妃杧的采后品质的影响,分别用10种植物精油对杧果炭疽病菌(Colletotrichum gloeosporioides)进行离体抑菌试验;以贵妃杧为试验材料,刺伤后分别用0.0、0.2、0.5、1.0、2.0和4.0 μL/mL的丁香精油浸泡5.0 min后接种杧果炭疽病菌,统计炭疽病病斑直径和病情指数;以2.0 μL/mL的丁香精油浸泡贵妃杧5.0 min后观察自然发病,统计病情指数和防治效果,同时研究了丁香精油对贵妃杧采后品质的影响。结果表明,离体抑菌试验中,10种供试植物精油对杧果炭疽病原菌的菌丝生体长均有明显的抑制效果,其中丁香精油的抑菌效果最好,EC50值和EC90值分别为0.19 μL/mL和0.57 μL/mL。在丁香精油对贵妃杧刺伤接种抑制效果试验中发现,贮藏12 d时,2.0 μL/mL处理的病斑直径及病情指数均显著低于对照,病斑直径为10.45 mm,病情指数为46.25。同时2.0 μL/mL处理能有效预防贵妃杧采后自然发病,且能显著抑制失重和果肉硬度下降,贮藏后期仍保持较高的可溶性固形物和维生素C含量(P<0.05),但对可滴定酸的影响不显著(P>0.05)。

本文引用格式

李俊国 , 胡美姣 , 弓德强 , 母军霞 , 王伟博 , 高兆银 , 李敏 , 谷会 , 陈千付 , 雷新涛 . 丁香精油对杧果采后炭疽病及品质的影响[J]. 食品与发酵工业, 2022 , 48(18) : 107 -113 . DOI: 10.13995/j.cnki.11-1802/ts.029074

Abstract

To explore the inhibitory effect of plant essential oils on Colletotrichum gloeosporioides after postharvest and the effect of plant essential oils on postharvest quality of ‘Guifei’ mango, the antifungal activity of 10 plant essential oils on Colletotrichum gloeosporioides was tested in vitro. Firstly, the wounded ‘Guifei’ mango was soaked in 0.2, 0.5, 1.0, 2.0 and 4.0 μL/mL clove essential oil for 5.0 min, and then inoculated with mango anthracnose. The lesion diameter and disease index of anthracnose were counted. The natural disease was observed after being soaked in 2.0 μL/mL clove essential oil for 5.0 min, and the disease index and control effect were recorded and the effects of treatment on the fruit quality were also studied. The results showed that 10 kinds of essential oils had significant inhibitory effects on the mycelial growth of mango anthracnose pathogen in the plate antibacterial test, and clove essential oil had the best inhibitory effect which with the EC50 and EC90 values of 0.19 μL/mL and 0.57 μL/mL, respectively. In the inhibitory effect of clove essential oil on the inoculation wounds of ‘Guifei’ mango, the lesion diameter and disease index of the 2.0 μL/mL treatment group were significantly lower than those of the control group at 12 d of storage. The lesion diameter was 10.45 mm and the disease index was 46.25. At the same time, keeping maintained high soluble solids and vitamin C content in the late storage 2.0 μL/mL treatment can effectively prevent the natural disease after harvest, which can significantly inhibit the weight loss and the decrease of flesh firmness (P<0.05),but had no significant effect on titratable acid (P>0.05).

参考文献

[1] CANNON P F, DAMM U, JOHNSTON P R, et al.Colletotrichum-current status and future directions[J].Studies in Mycology, 2012,73:181-213.
[2] DAMM U, CANNON P F, WOUDENBERG J H C, et al.The Colletotrichum acutatum species complex[J].Studies in Mycology, 2012,73:37-113.
[3] DAMM U, CANNON P F, WOUDENBERG J H C, et al.The Colletotrichum boninense species complex[J].Studies in Mycology, 2012,73:1-36.
[4] 谢冬微, 路颖, 杨学, 等.植物抗炭疽病相关基因的研究进展[J].华北农学报, 2015,30(S1):348-354.
XIE D W, LU Y, YANG X, et al.Research progress on related genes of resistance to anthracnose in plant[J].Acta Agriculture Boreali-Sinica, 2015,30(S1):348-354.
[5] 杨建波, 欧阳秋飞, 黄战威.杧果采后主要真菌病害及其抗病机理研究进展[J].热带农业科学, 2019,39(10):106-110.
YANG J B, OU YANG Q F, HUANG Z W.Research advances in main postharvest fungal diseases and their resistance mechanisms in mango (Mangifera indica L.) [J].Chinese Journal of Tropical Agriculture, 2019,39(10):106-110.
[6] 刘威, 袁丁, 郭桂义, 等.茶树炭疽病病原鉴定[J].南方农业学报, 2017,48(3):448-453.
LIU W, YUAN D, GUO G Y, et al.Identification of anthracnose pathogen in tea plant[J].Journal of Southern Agriculture, 2017,48(3):448-453.
[7] 冉飞,高强,龙友华, 等.贵州百香果炭疽病病原鉴定[J].植物病理学报, 2021.https://doi.org/10.13926/j.cnki.apps.000433.
RAN F, GAO Q, LONG Y H, et al.Identification of the pathogen causing anthracnose of passion fruit in Guizhou Province[J].Acta Phytopathologica Sinica, 2021.https://doi.org/10.13926/j.cnki.apps.000433.
[8] 周子骞, 李敏, 高兆银, 等.海南番木瓜炭疽病病原菌鉴定及其防治药剂筛选[J].西南农业学报, 2020,33(1):70-76.
ZHOU Z Q, LI M, GAO Z Y, et al.Pathogens identification and fungicide screening of papaya fruit anthracnose in Hainan[J].Southwest China Journal of Agricultural Sciences, 2020,33(1):70-76.
[9] 杨波, 王卉, 杭瑜瑜, 等.精油固体保鲜剂对芒果保鲜效果的研究[J].科技视界, 2016(16):57-58.
YANG B, WANG H, HANG Y Y, et al.Study on preservation effect on mango by essential oil solid preservatives[J].Science & Technology Vision, 2016(16):57-58.
[10] SANTOS-VILLALOBOS S D L, et al.Critical aspects on the integral management of mango:Flowering, anthracnosis and industrial waste[J].Revista Mexicana de Ciencias Agrícolas,2011, 2(2):221-234.
[11] 李晓娜, 曾小红, 谢龙莲, 等.世界芒果炭疽病防治技术研究概况[J].热带农业科学, 2017,37(11):69-75.
LI X N, ZENG X H, XIE L L, et al.The review on anthracnose prevention and control technology of mango[J].Chinese Journal of Tropical Agriculture, 2017,37(11):69-75.
[12] HU M J, YANG D P, HUBER D J, et al.Reduction of postharvest anthracnose and enhancement of disease resistance in ripening mango fruit by nitric oxide treatment[J].Postharvest Biology and Technology, 2014,97:115-122.
[13] 韩旭旭, 王玉涵, 王鑫.植物精油在果蔬保鲜领域的应用研究及展望[J].食品研究与开发, 2018,39(23):204-208.
HAN X X, WANG Y H, WANG X.Research and prospect of the application of plant essential oil in the field of fruit and vegetable preservation[J].Food Research and Development, 2018,39(23):204-208.
[14] 潘小军, 高阳, 翁甜.植物精油对黄瓜贮藏效果的影响[J].食品与发酵工业, 2020,46(20):187-192.
PAN X J, GAO Y, WENG T.Study on the effects of plant essential oils on cucumber storage[J].Food and Fermentation Industries, 2020,46(20):187-192.
[15] 林铭杰, 张建桃, 宋庆奎, 等.雾化植物精油在荔枝保鲜中的应用研究[J].现代农业科技, 2021(6):202-205;215.
LIN M J, ZHANG J T, SONG Q K, et al.Study on the application of atomized plant essential oil in litchi preservation[J].Modern Agricultural Science and Technology, 2021(6):202-205;215.
[16] 林树花, 王伟, 张菊华, 等.10种植物精油对蓝莓病原菌的体外抑菌活性初筛[J].农业科学与技术(英文版), 2019, 20(3):17-21.
LIN S H, WANG W, ZHANG J H, et al.Initial screening of 10 plant essential oils in vitro antibacterial activity against blueberry pathogens[J].Agricultural Science & Technology, 2019,20(3):17-21.
[17] 张娜, 关文强, 阎瑞香.芥末精油对芒果采后病原真菌抑制效果和保鲜效应的研究[J].食品工业科技, 2011,32(3):349-353.
ZHANG N, GUAN W Q, YAN R X.Effects of mustard essential oil on postharvest pathogens and quality of mango[J].Science and Technology of Food Industry, 2011,32(3):349-353.
[18] 陈梅. 丁香精油对番茄采后腐烂抑制作用及成分分析[J].山西农业大学学报(自然科学版), 2016,36(9):678-684.
CHEN M.Inhibitory effects of clove oil on postharvest decay of tomato and its essential components[J].Journal of Shanxi Agricultural University (Natural Science Edition), 2016,36(9):678-684.
[19] 覃丽萍, 苏琴, 汪茜, 等.广西杧果炭疽病病菌致病力差异测定[J].中国植保导刊, 2016,36(4):5-8.
QIN L P, SU Q, WANG Q, et al.Determination on pathogenicity differences of mango anthracnose pathogens[J].China Plant Protection, 2016,36(4):5-8.
[20] 弓德强, 梁清志, 黄光平, 等.BABA处理对采后芒果果实抗病性的影响[J].热带作物学报, 2015,36(11):2 067-2 072.
GONG D Q, LIANG Q Z, HUANG G P, et al.Effect of BABA on disease resistance in harvested mango fruits[J].Chinese Journal of Tropical Crops, 2015,36(11):2 067-2 072.
[21] 王丹, 张静, 贾晓曼, 等.丁香精油对甜樱桃采后优势致腐真菌的控制及其抑菌机理[J].核农学报, 2020,34(6):1 221-1 229.
WANG D, ZHANG J, JIA X M, et al.Antifungal activity and possible mechanism of clove essential oil on dominant pathogens of postharvest sweet cherries[J].Journal of Nuclear Agricultural Sciences, 2020, 34(6):1 221-1 229.
[22] 李宁, 关文强, 赵丽静.丁香精油对贮藏期圣女果防腐效果初步研究[J].天津农学院学报, 2011, 18(2):16-19.
LI N, GUAN W Q, ZHAO L J.Preliminary study on clove oil preventing decay of cherry tomato during storage[J].Journal of Tianjin Agricultural University, 2011,18(2):16-19.
[23] 李鹏霞, 邵世达, 冯俊涛, 等.丁香精油和丁香酚对苹果贮藏期病害及果实品质的影响[J].农业工程学报, 2006, 22(6):173-177.
LI P X, SHAO S D, FENG J T, et al.Effects of essential oil and phenolic extracted from clove leaves(Syringa portolaciniata) on postharvest apple pathogens and quality[J].Transactions of the CSAE, 2006, 22(6):173-177.
[24] 李宁,关文强,阎瑞香.丁香精油对冷藏冬枣果实腐烂及诱导抗病相关酶活性的影响[J].西北植物学报, 2012,32(2):324-329.
LI N, GUAN W Q, YAN R X.Inhibition of postharvest decay in relation to induced disease resistance of Dongzao jujube fruit treated with clove oil during cold storage and shelf period [J].Acta Botanica Boreali-Occidentalia Sinica, 2012,32(2):324-329.
[25] 黄巍, 王建清, 高康, 等.丁香精油涂布纸箱对水蜜桃的保鲜效果[J].包装工程, 2017,38(9):25-30.
HUANG W, WANG J Q, GAO K, et al.Fresh-keeping effect of carton coated with clove essential oil on juicy peaches[J].Packaging Engineering, 2017,38(9):25-30.
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