分析与检测

重庆针形名优绿茶香气特征及成分分析

  • 杨娟 ,
  • 徐泽 ,
  • 吴全 ,
  • 钟应富 ,
  • 王杰 ,
  • 罗红玉 ,
  • 袁林颖
展开
  • (重庆市农业科学院茶叶研究所,重庆市茶叶工程技术研究中心,重庆,402160)
第一作者:硕士,助理研究员(袁林颖研究员为通信作者,E-mail:350129893@qq.com)

收稿日期: 2021-07-01

  修回日期: 2021-09-27

  网络出版日期: 2022-10-17

基金资助

重庆市科技局科研机构绩效激励引导专项(cstc2020jxjl80014);重庆市科技局技术创新与应用示范专项(cstc2018jscx-mszdX0032);“科技助力经济2020”重点专项(SQ2020YFF0422398)

Analysis of aroma characteristics and components of Chongqing famous needle shaped green tea

  • YANG Juan ,
  • XU Ze ,
  • WU Quan ,
  • ZHONG Yingfu ,
  • WANG Jie ,
  • LUO Hongyu ,
  • YUAN Linying
Expand
  • (Tea Research Institute of Chongqing Academy of Agricultural Sciences, Chongqing Engineering Research Center for Tea, Chongqing 402160, China)

Received date: 2021-07-01

  Revised date: 2021-09-27

  Online published: 2022-10-17

摘要

为探究重庆针形名优绿茶香气品质特征及成分组成,收集重庆市38个针形名优绿茶样品,采用感官审评及顶空固相微萃取联用气相色谱-质谱技术对其香气特征及含量进行分析。结果表明,重庆针形名优绿茶主要表现为清香型或栗香型。共检测出香气成分40种,包括醇类11种,酯类6种,醛类4种,酮类5种,碳氢化合物类10种,芳烃2种,杂环及含硫化合物各1种。香叶醇、苯甲醇、苯乙醇、β-芳樟醇、顺-茉莉酮、吲哚、橄榄醇、橙花叔醇、顺-已酸-3-己烯酯、2-甲基戊酸甲酯、水杨酸甲酯、β-杜松烯、顺-β-法尼烯等香气物质对构成重庆针形名优绿茶特征香气品质具有重要作用。酯类在清香型绿茶中含量显著高于栗香型绿茶。通过主成分分析,清香型与栗香型绿茶主要贡献香气成分有明显差异,清香型绿茶差异成分有反,反-3,5-辛二烯-2-酮、柠檬烯、反-戊酸-2-己烯酯、香叶基丙酮、橙花叔醇、氧化芳樟醇Ⅰ(呋喃型)、β-芳樟醇、顺-茉莉酮、6,10,14-三甲基-十烷-2-酮,主要为呈现花香和果香类物质;栗香型绿茶差异成分主要有环氧芳樟醇Ⅱ、4,8-二甲基-1,3,7-壬三烯、壬醛、二甲硫,主要为呈现甜香和鲜味类物质。产地聚类分析表明,地区微域环境对重庆针形名优绿茶香气品质形成有重要影响,其中永川区所产永川秀芽的聚类效果最好。该研究可为重庆针形名优绿茶特征香气形成研究及品质评价提供理论参考。

关键词: 绿茶; 香气; 特征; 成分

本文引用格式

杨娟 , 徐泽 , 吴全 , 钟应富 , 王杰 , 罗红玉 , 袁林颖 . 重庆针形名优绿茶香气特征及成分分析[J]. 食品与发酵工业, 2022 , 48(18) : 256 -264 . DOI: 10.13995/j.cnki.11-1802/ts.028521

Abstract

To explore the aroma characteristics and compositions of Chongqing famous needle shaped green tea, thirty-eight samples of Chongqing famous needle green tea were collected as raw materials. The aroma components were extracted by headspace solid phase microextraction (HS-SPME) and analyzed by gas chromatography-mass spectrometry (GC/MS) coupled with sensory evaluation. The result showed that clean and refreshing type and chestnut aroma type were the two main aromatic types of Chongqing famous needle shaped green tea. Totally, forty aroma components could be detected including eleven alcohols, six esters, four aldehydes, five ketones, ten hydrocarbons, two aromatics, one nitrogen-containing and one sulfur-containing compound. Geraniol, benzyl alcohol, phenylethanol β-linalool, CIS jasmonate, indole, olive alcohol, nerolidol, cis-3-hexenyl hexanoate, methyl 2-methylvalerate, methyl salicylate β-terpinene, CIS-β-farnesene, etc. These components also played important roles in the aromatic characteristics of Chongqing famous needle shaped green tea. The content of esters in clean and refreshing green tea was significantly higher than that in chestnut aroma green tea. Moreover, significant differences in the main aromatic components between clean and refreshing green tea and chestnut aroma green tea were identified by PCA analysis. The different components of clean and refreshing green tea include anti-3,5-octylene-2-one, limonene, trans-pentanoate-2-hexene ester, vanillin acetone, nerhuatert alcohol, linalool oxide I (furan type) β-linalool, cis jasmonane, 6,10,14-trimethyl-decane-2-one, which were mainly floral and fruity. The different components of chestnut aroma green tea were epoxy linalool II, 4,8-dimethyl-1,3,7-nonatriene, nonanal and dimethylsulfide, which were sweet and fresh. Furthermore, in the cluster analysis results of product areas, regional micro-environment had an important effect on the formation of aroma characteristics of Chongqing famous needle shaped green tea, and the clustering effect of Yongchuan Xiuya was the best. This study could provide a theoretical reference for the characteristic aroma formation and quality evaluation of Chongqing famous needle shaped green tea.

参考文献

[1] YANG Z Y, BALDERMANN S, WATANABE N.Recent studies of the volatile compounds in tea[J].Food Research International, 2013, 53(2):585-599.
[2] 张铭铭, 江用文, 袁海波, 等.绿茶栗香的形成及工艺研究进展[J].中国农学通报, 2020, 36(2):129-137.
ZHANG M M, JIANG Y W, YUAN H B, et al.Chestnut-like aroma of green tea:Research progress of formation and technology[J].Chinese Agricultural Science Bulletin, 2020, 36(2):129-137.
[3] 袁海波, 尹军峰, 叶国柱, 等.茶叶香型及特征物质研究进展[J].中国茶叶, 2009, 31(8):14-15.
YUAN H B, YIN J F, YE G Z, et al.Research advances on aromatic components of tea[J].China Tea, 2009, 31(8):14-15.
[4] 尹洪旭. 栗香绿茶特征香气成分研究[D].北京:中国农业科学院, 2018.
YIN H X.Studies on characteristics volatile components of green tea with chestnut aroma[D].Beijing:Chinese Academy of Agricultural Sciences, 2018.
[5] 张铭铭, 尹洪旭, 邓余良, 等.基于HS-SPME/GC×GC-TOFMS/OAV不同栗香特征绿茶关键香气组分分析[J].食品科学, 2020, 41(2):244-252.
ZHANG M M, YIN H X, DENG Y L, et al.Analysis of key odorants responsible for different chestnut-like aromas of green teas based on headspace solid-phase microextraction coupled with comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry and odor activity value[J].Food Science, 2020, 41(2):244-252.
[6] 黄海涛, 敖存, 郭敏明, 等.花香与清香绿茶的香气组分及品种相关性分析[J].浙江农业科学, 2018, 59(5):738-741;771.
HUANG H T, AO C, GUO M M, et al.Study on aroma components of scent-flavor and floral-flavor tea and its correlation analysis[J].Journal of Zhejiang Agricultural Sciences, 2018, 59(5):738-741;771.
[7] LIAO X L, YAN J N, WANG B, et al.Identification of key odorants responsible for cooked corn-like aroma of green teas made by tea cultivar ‘ Zhonghuang 1’[J].Food Research International, 2020, 136:109355.
[8] 汪琦, 赵贵福, 张续周, 等.4个茶树品种与季节对绿茶香气成分特征的影响[J].食品科学, 2016, 37(20):102-107.
WANG Q, ZHAO G F, ZHANG X Z, et al.Effects of four different tea varieties and harvesting seasons on aroma characteristics of green tea[J].Food Science, 2016, 37(20):102-107.
[9] 苏敏, 韦柳花, 刘初生, 等.不同季节修仁品种茶叶香气成分的GC-MS分析[J].西南农业学报, 2017, 30(7):1 538-1 545.
SU M, WEI L H, LIU C S, et al.Aroma-producing components of Xiuren tea leaves during different seasons by GC-MS[J].Southwest China Journal of Agricultural Sciences, 2017, 30(7):1 538-1 545.
[10] 赖幸菲, 潘顺顺, 李裕南, 等.不同季节和茶类的金萱品种茶叶香气成分分析[J].食品工业科技, 2015, 36(10):62-68;72.
LAI X F, PAN S S, LI Y N, et al.Analysis of aromatic components in Jinxuan tea of different kinds and seasons[J].Science and Technology of Food Industry, 2015, 36(10):62-68;72.
[11] 康受姈. 不同茶树品种、生产季节和加工方法对茶叶挥发性化合物的影响[D].杭州:浙江大学, 2016.
KANG S Y.Effects of tea cultivar, growing season and processing method on volatiles of tea leaves[D].Hangzhou:Zhejiang University, 2016.
[12] 董迹芬, 边金霖, 朱全武, 等.茶叶香气与产地土壤条件的关系[J].浙江大学学报(农业与生命科学版), 2013, 39(3):309-317.
DONG J F, BIAN J L, ZHU Q W, et al.Relationship between tea aroma and soil conditions[J].Journal of Zhejiang University (Agriculture and Life Sciences), 2013, 39(3):309-317.
[13] 王秋霜, 吴华玲, 姜晓辉, 等.基于多元统计分析方法的广东罗坑红茶香气品质研究[J].现代食品科技, 2016, 32(2):309-316.
WANG Q S, WU H L, JIANG X H, et al.Multivariate statistical analysis of the aroma qualities of black tea from Luokeng Guangdong[J].Modern Food Science and Technology, 2016, 32(2):309-316.
[14] 坤吉瑞, 闫敬娜, 舒娜, 等.不同日晒技术对晒青绿茶中挥发性化合物、脂肪酸和感官品质的影响[J].食品与发酵工业, 2020, 46(21):154-160.
KUN J R, YAN J N, SHU N, et al.Effect of different sun drying techniques on fatty acids, volatile compounds and sensory quality of green tea[J].Food and Fermentation Industries, 2020, 46(21):154-160.
[15] 张铭铭, 江用文, 滑金杰, 等.干燥方式对绿茶栗香的影响[J].食品科学, 2020, 41(15):115-123.
ZHANG M M, JIANG Y W, HUA J J, et al.Effect of drying methods on chestnut-like aroma of green tea[J].Food Science, 2020, 41(15):115-123.
[16] 王奕, 罗红玉, 袁林颖, 等.不同干燥方式对夏季绿茶香气品质的影响[J].食品工业科技, 2021, 42(9):1-9.
WANG Y, LUO H Y, YUAN L Y, et al.Effects of drying methods on volatile components of summer green tea[J].Science and Technology of Food Industry, 2021, 42(9):1-9.
[17] CHIEN H J, YANG M M, WANG W C, et al.Proteomic analysis of "Oriental Beauty" oolong tea leaves with different degrees of leafhopper infestation[J].Rapid Communications in Mass Spectrometry, 2020, 34(15):e8825.
[18] 闫振, 王登良.茶叶香气物质响应胁迫机制与功能研究进展[J].食品科学, 2021, 42(15):337-345.
YAN Z, WANG D L.Progress in understanding the stress response mechanisms and functions of aroma compounds in tea[J].Food Science, 2021, 42(15):337-345.
[19] ZHANG M M, YANG Y Q, YUAN H B, et al.Contribution of addition theanine/sucrose on the formation of chestnut-like aroma of green tea[J].LWT, 2020, 129:109512.
[20] WANG M Q, MA W J, SHI J, et al.Characterization of the key aroma compounds in Longjing tea using stir bar sorptive extraction (SBSE) combined with gas chromatography-mass spectrometry (GC-MS), gas chromatography-olfactometry (GC-O), odor activity value (OAV), and aroma recombination[J].Food Research International, 2020, 130:108908.
[21] 樊荣辉, 黄敏玲, 钟淮钦, 等.花香的生物合成、调控及基因工程研究进展[J].中国细胞生物学学报, 2011, 33(9):1 028-1 036.
FAN R H, HUANG M L, ZHONG H Q, et al.Advances in biosynthesis, regulation and genetic engineering of floral scent[J].Chinese Journal of Cell Biology, 2011, 33(9):1 028-1 036.
[22] 任倩倩, 庄明珠, 蔡晓明, 等.小贯小绿叶蝉取食诱导抗、感茶树品种挥发物的释放[J].茶叶科学, 2020, 40(6):795-806.
REN Q Q, ZHUANG M Z, CAI X M, et al.The release of volatiles in resistant and susceptible tea cultivars under Empoasca onukii feeding[J].Journal of Tea Science, 2020, 40(6):795-806.
[23] BALDERMANN S, YANG Z Y, KATSUNO T, et al.Discrimination of green, oolong, and black teas by GC-MS analysis of characteristic volatile flavor compounds[J].American Journal of Analytical Chemistry, 2014, 5(9):620-632.
[24] ZHU J C, NIU Y W, XIAO Z B.Characterization of the key aroma compounds in Laoshan green teas by application of odour activity value (OAV), gas chromatography-mass spectrometry-olfactometry (GC-MS-O) and comprehensive two-dimensional gas chromatography mass spectrometry (GC×GC-qMS)[J].Food Chemistry, 2021, 339:128136.
[25] 廉明, 吕世懂, 吴远双, 等.茶叶挥发性成分形成机制的研究进展[J].生命科学研究, 2014, 18(6):550-556.
LIAN M, LYU S D, WU Y S, et al.Progresses on the formation mechanism of the volatile component in tea[J].Life Science Research, 2014, 18(6):550-556.
[26] 汪蓓. 杀青与提香温度对绿茶香型及其特征香气影响的研究[D].重庆:西南大学, 2020.
WANG B.Study on the effects of pan-firing and baking temperature on the aroma type and characteristic of green tea[D].Chongqing:Southwest University, 2020.
[27] 董晨, 何畅, 朱珺语, 等.不同做形方式对鄂茶10号优质绿茶品质的影响[J].云南农业大学学报(自然科学), 2020, 35(1):139-148.
DONG C, HE C, ZHU J Y, et al.Effect of shaping methods on the quality of E-Cha No.10 green tea products[J].Journal of Yunnan Agricultural University (Natural Science), 2020, 35(1):139-148.
[28] 蔺志远, 施江, 谭俊峰, 等.提香温度对绿茶(香茶)干燥效能及风味品质的影响[J].食品科学, 2020, 41(23):153-158.
LIN Z Y, SHI J, TAN J F, et al.Effect of drying temperature on drying efficiency, energy consumption and flavor quality of Xiangcha, a kind of green tea[J].Food Science, 2020, 41(23):153-158.
[29] 叶伟华, 李杰峰.海拔对乌牛早茶叶品质的影响[J].浙江农业科学, 2020, 61(8):1 512-1 516.
YE W H, LI J F.Effect of altitude on tea quality of Wuniuzao[J].Journal of Zhejiang Agricultural Sciences, 2020, 61(8):1 512-1 516.
[30] 郝麒麟, 陈梅, 贺燕, 等.基于因子综合法评价重庆X区绿茶品质[J].食品与发酵工业, 2020, 46(10):278-283.
HAO Q L, CHEN M, HE Y, et al.Evaluation of green tea quality from Chongqing X district based on comprehensive factor method[J].Food and Fermentation Industries, 2020, 46(10):278-283.
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