该文以聚丙烯酰胺凝胶膜为萃取材料,利用自行组装的萃取装置,建立了一种基于电膜萃取法(electromembrane extraction,EME)快速提取奶酪中组胺的方法。在电场驱动力作用下,组胺阳离子经聚丙烯凝胶膜孔进入接受相溶液中,采用HPLC对萃取液进行分析。结果表明组胺富集倍数达到1.7倍,相对标准偏差(n=6)为1.41%~5.71%。该方法操作简单、成本低,能够满足组胺的快速分析需求,也为食品中其他无机化学危害物的提取与检测提供了新的方法与思路。
A rapid histamine extraction method in cheese was established using a self-prepared electromembrane extraction (EME) instrument, in which a polyacrylamide gel was used as the extraction membrane. Driven by the force of the electric field, the histamine cation enters into the acceptor solution through the hole of polyacrylamide gel, and the extract was analyzed by the HPLC. The results showed that the enrichment factor of histamine reached 1.7 times with relative standard deviations (n=6) ranging from 1.41% to 5.71%. The method is simple in operation and low in cost, which can meet the demand of rapid detection of histamine, and also provides a new method for the extraction and detection of other inorganic chemical hazards in foods.
[1] SUZZI G, TORRIANI S.Editorial:Biogenic amines in foods[J].Frontiers in Microbiology, 2015, 6:472.
[2] VALSAMAKI K, MICHAELIDOU A, POLYCHRONIADOU A.Biogenic amine production in Feta cheese[J].Food Chemistry, 2000, 71(2):259-266.
[3] NAM J H, CHO Y S, RACKERBY B, et al.Shifts of microbiota during cheese production:Impact on production and quality[J].Applied Microbiology and Biotechnology, 2021, 105(6):2 307-2 318.
[4] 金高娃, 蔡友琼, 于慧娟, 等.柱前衍生高效液相色谱法测定鱼罐头中的组胺[J].色谱, 2010, 28(11):1 099-1 102.
JIN G W, CAI Y Q, YU H J, et al.Determination of histamine in canned fish by high performance liquid chromatography with pre-column derivatization[J].Chinese Journal of Chromatography, 2010, 28(11):1 099-1 102.
[5] PAPAGEORGIOU M, LAMBROPOULOU D, MORRISON C, et al.Literature update of analytical methods for biogenic amines determination in food and beverages[J].TrAC Trends in Analytical Chemistry, 2018, 98:128-142.
[6] MADEJSKA A, MICHALSKI M, PAWUL-GRUBA M, et al.Histamine content in rennet ripening cheeses during storage at different temperatures and times[J].Journal of Veterinary Research, 2018, 62(1):65-69.
[7] 何璇, 马堃, 哈斯, 等.食品中生物胺形成与抑制的研究进展[J].食品与发酵工业, 2021, 47(18):294-300.
HE X, MA K, HA S, et al.Research progress on the formation and inhibition of biogenic amines in food[J].Food and Fermentation Industries, 2021, 47(18):294-300.
[8] 王光强, 俞剑燊, 胡健, 等.食品中生物胺的研究进展[J].食品科学, 2016, 37(1):269-278.
WANG G Q, YU J S, HU J, et al.Progress in research on biogenic amines in foods[J].Food Science, 2016, 37(1):269-278.
[9] SENTELLAS S, NÚÑEZ Ó, SAURINA J.Recent advances in the determination of biogenic amines in food samples by (U)HPLC[J].Journal of Agricultural and Food Chemistry, 2016, 64(11):7 667-7 678.
[10] PAULO VIEIRA C, PEREIRA DA COSTA M, SILVA V L M, et al.Development and validation of RP-HPLC-DAD method for biogenic amines determination in probiotic yogurts[J].Arabian Journal of Chemistry, 2020, 13(1):1 582-1 597.
[11] GIL R L, AMORIM C G, MONTENEGRO M C B S M, et al.HPLC-potentiometric method for determination of biogenic amines in alcoholic beverages:A reliable approach for food quality control[J].Food Chemistry, 2022, 372:131288.
[12] SANTIGOSA-MURILLO E, MASPOCH S, MUÑOZ M, et al.An efficient microfluidic device based on electromembrane extraction for the simultaneous extraction of acidic and basic drugs[J].Analytica Chimica Acta, 2021, 1 160:338448.
[13] ABBASI S, HAERI S A.Enrichment of psychotropic drugs using rhamnolipid bioaggregates after electromembrane extraction based on an agarose gel using a rotating electrode as a green and organic solvent-free strategy[J].Journal of Chromatography A, 2021, 1 655:462500.
[14] KAMANKESH M, MOHAMMADI A, MOLLAHOSSEINI A, et al.Application of a novel electromembrane extraction and microextraction method followed by gas chromatography-mass spectrometry to determine biogenic amines in canned fish[J].Analytical Methods, 2019, 11(14):1 898-1 907.
[15] GJELSTAD A, RASMUSSEN K E, PEDERSEN-BJERGAARD S.Electrokinetic migration across artificial liquid membranes:Tuning the membrane chemistry to different types of drug substances[J].Journal of Chromatography A, 2006, 1 124(1-2):29-34.
[16] 魏欣,李青莲.电膜微萃取在样品前处理中的研究进展[J].药物分析杂志, 2017, 37(12):2 133-2 138.
WEI X, LI Q L.Research progress of electromembrane microextraction in the pretreatment of samples[J].Chinese Journal of Pharmaceutical Analysis, 2017, 37(12):2 133-2 138.
[17] ASADI S, TABANI H, NOJAVAN S.Application of polyacrylamide gel as a new membrane in electromembrane extraction for the quantification of basic drugs in breast milk and wastewater samples[J].Journal of Pharmaceutical and Biomedical Analysis, 2018, 151:178-185.
[18] ASADI S, NOJAVAN S, BEHPOUR M, et al.Electromembrane extraction based on agarose gel for the extraction of phenolic acids from fruit juices[J].Journal of Chromatography B, 2020, 1 159:122401.
[19] SAFARI M, NOJAVAN S, DAVARANI S S H, et al.Speciation of chromium in environmental samples by dual electromembrane extraction system followed by high performance liquid chromatography[J].Analytica Chimica Acta, 2013, 789:58-64.
[20] MIDDELTHON-BRUER T M, GJELSTAD A, RASMUSSEN K E, et al.Parameters affecting electro membrane extraction of basic drugs[J].Journal of Separation Science, 2008, 31(4):753-759.
[21] BALCHEN M, JENSEN H, REUBSAET L, et al.Potential-driven peptide extractions across supported liquid membranes:Investigation of principal operational parameters[J].Journal of Separation Science, 2010, 33(11):1 665-1 672.
[22] DROUIN N, KUBÁŇ P, RUDAZ S, et al.Electromembrane extraction:Overview of the last decade[J].TrAC Trends in Analytical Chemistry, 2019, 113:357-363.
