研究了植物激素2,4-二氯苯氧乙酸(2,4-dichlorophenoxyacetic acid,2,4-D)与盐胁迫联合培养对小球藻(Chlorella vulgaris)生长、产脂的影响,并进一步分析了小球藻的生理生化指标及脂肪酸成分的变化。结果表明,在2,4-D与盐胁迫的联合处理下,小球藻的生物量与之单独盐胁迫条件下相比最高可提高55.45%,油脂含量也进一步提高了10.47%,所得最大油脂生产力比单独盐胁迫时高出65.8%。在10 g/L NaCl与2 mg/L 2,4-D的培养条件下,随培养时间增加,小球藻的叶绿素a、类胡萝卜素以及可溶性蛋白含量上升,碳水化合物含量呈先下降后上升的趋势,饱和脂肪酸含量也达到了37.79%。试验通过植物激素与盐胁迫联合的方式实现了小球藻快速生长与高效率积累油脂相结合,为规模化生产生物燃料奠定了基础。
王依霖
,
莫创荣
,
许雪棠
,
黄丽珍
,
谭顺
,
陆祖逸
,
王露洁
,
庞瑞林
. 2,4-二氯苯氧乙酸与盐胁迫联合提高小球藻的产脂量[J]. 食品与发酵工业, 2022
, 48(24)
: 105
-110
.
DOI: 10.13995/j.cnki.11-1802/ts.031174
The effects of plant hormone 2,4-dichlorophenoxyacetic acid (2,4-D) combined with salt stress on the growth and lipid production of Chlorella vulgaris were studied, and the changes of physiological and biochemical indexes and fatty acid composition of C. vulgaris were further analyzed. The results showed that under the combined treatment of 2,4-D and salt stress, the biomass of C. vulgaris was increased by 55.45% and the oil content was further increased by 10.47% compared with that under salt stress alone. The maximum oil productivity was 65.8% higher than that under salt stress alone. Under the culture conditions of 10 g/L NaCl and 2 mg/L 2,4-D, with the increase of culture time, the contents of chlorophyll a, carotenoid and soluble protein of C. vulgaris increased, the content of carbohydrate decreased first and then increased, and the content of saturated fatty acid also reached 37.79%. Through the combination of plant hormones and salt stress, the experiment realized the combination of rapid growth of C. vulgaris and efficient accumulation of oil, which laid the foundation for large-scale production of biofuels.
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