食品与发酵工业 >

2022 , Vol. 48 >Issue 24: 220 - 225

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.030165

研究报告

灵芝双向发酵液对于HaCaT细胞中波紫外线损伤的修复研究

  • 张影 ,
  • 孟宪瑶 ,
  • 杨云丽 ,
  • 郭苗苗 ,
  • 周卫强 ,
  • 李丽
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  • 1(北京工商大学 化学与材料工程学院,北京,100048)
    2(北京市植物资源研究开发重点实验室,北京,100048)
    3(中粮营养健康研究院有限公司,北京,102209)
第一作者：硕士研究生(李丽教授为通信作者,E-mail:lili2212@163.com)

收稿日期: 2021-11-29

  修回日期: 2022-03-26

  网络出版日期: 2023-01-06

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Repair of UVB light damage in HaCaT cells by Ganoderma lucidum bidirectional fermentation broth

  • ZHANG Ying ,
  • MENG Xianyao ,
  • YANG Yunli ,
  • GUO Miaomiao ,
  • ZHOU Weiqiang ,
  • LI Li
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  • 1(College of Chemistry and Materials Engineering, Beijing Technology & Business University, Beijing 100048, China)
    2(Beijing Key Lab of Plant Resource Research and Development, Beijing 100048, China)
    3(Nutrition & Health Research Institute, COFCO Corporation, Beijing 102209, China)

Received date: 2021-11-29

  Revised date: 2022-03-26

  Online published: 2023-01-06

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摘要

该研究通过液体深层发酵制备灵芝、灵芝-玉竹、灵芝-桦树茸、灵芝-人参以及灵芝-三七发酵液冻干粉,MTT细胞毒性实验及细胞形态学检测筛选出样品给药浓度,建立300 mJ/cm2中波紫外线(ultraviolet radiation b,UVB,290~320 nm)辐射人角质形成细胞(human immortalized keratinocytes,HaCaT)的损伤模型,酶联免疫吸附测定(enzyme linked immunosorbent assay,ELISA)法检测HaCaT细胞分泌炎症因子白细胞介素-1α(interleukin-1α,IL-1α)及前列腺素E2(prostaglandinE2,PGE2)的含量。以此来探究灵芝、灵芝-玉竹等发酵液冻干粉对UVB诱导的HaCaT细胞光损伤的修复作用,筛选出最优的灵芝发酵组合方式。研究结果发现,相比于灵芝发酵液冻干粉,灵芝-桦树茸发酵液冻干粉对IL-1α及PGE2的分泌有显著的抑制作用(P<0.05)。综上,相较于灵芝发酵液冻干粉,灵芝-桦树茸发酵液冻干粉修复HaCaT细胞UVB光损伤的能力最强,因此通过添加桦树茸进行灵芝发酵为最优的发酵组合方式,这为灵芝组合发酵的工业化生产提供了前提和基础。

关键词： 灵芝; 发酵; HaCaT细胞; 中波紫外线; 光损伤

本文引用格式

张影 , 孟宪瑶 , 杨云丽 , 郭苗苗 , 周卫强 , 李丽 . 灵芝双向发酵液对于HaCaT细胞中波紫外线损伤的修复研究[J]. 食品与发酵工业, 2022 , 48(24) : 220 -225 . DOI: 10.13995/j.cnki.11-1802/ts.030165

Abstract

The aim of this study is to explore the repair effects of Ganoderma lucidum, G. lucidum-Polygonatum odoratum, G. lucidum-Inonotus obliquus, G. lucidum-Panax ginseng and G. lucidum-Panax notoginseng fermentation broth freeze-dried powder on human keratinocytes (HaCaT) photodamage induced by UVB, and to screen out the optimal fermentation combination of G. lucidum. Lyophilized powder of G. lucidum, G. lucidum-P. odoratum was prepared by liquid deep fermentation. MTT cytotoxicity assay and cell morphology were used to screen out the concentration of the sample. The damage model of HaCaT cells under 300 mJ/cm2 UVB radiation was established. The levels of interleukin-1α (IL-1α) and prostaglandinE2 (PGE2) secreted by HaCaT cells were determined by enzyme linked immunosorbent assay (ELISA). The results showed that compared with the lyophilized powder of G. lucidum fermentation broth, the lyophilized powder of G. lucidum-I. obliquus fermentation had significant inhibitory effect on the secretion of IL-1α and PGE2 (P<0.05). Therefore, this paper concluded that compared with the lyophilized powder of G. lucidum fermentation liquid, the lyophilized powder of G. lucidum-I. obliquus fermentation liquid has the strongest ability to repair UVB light damage of HaCaT cells. Therefore, the optimal fermentation combination method of G. lucidum-I. obliquus fermentation is the best, which provides the premise and basis for the industrial production of G. lucidum fermentation combination.

Key words： Ganoderma lucidum; fermentation; HaCaT cells; UVB rays; photodamage

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