食品与发酵工业 >

2022 , Vol. 48 >Issue 24: 245 - 251

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.030338

分析与检测

四极杆-轨道阱液质联用法同时检测玉米中四种真菌毒素

  • 吴红涛 ,
  • 李萌萌 ,
  • 关二旗 ,
  • 刘远晓 ,
  • 王瑞虎 ,
  • 卞科
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  • (河南工业大学 粮油食品学院,河南 郑州,450000)
第一作者：硕士研究生(李萌萌讲师和卞科教授为共同通信作者,E-mail:limeng112578@126.com;kebian_lysp@haut.edu.cn)

收稿日期: 2021-12-10

  修回日期: 2022-01-13

  网络出版日期: 2023-01-06

基金资助

十三五国家重点研发计划战略性国际科技创新合作重点专项“粮食产业链中真菌毒素等污染物监测预警及防控关键技术的集成与应用”(2018YFE0206000);2020年河南工业大学青年骨干教师培育计划

收起

Simultaneous determination of four mycotoxins in maize by quadrupole-orbitrap LC-MS

  • WU Hongtao ,
  • LI Mengmeng ,
  • GUAN Erqi ,
  • LIU Yuanxiao ,
  • WANG Ruihu ,
  • BIAN Ke
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  • (School of Food Science and Technology, Henan University of Technology, Zhengzhou 450000, China)

Received date: 2021-12-10

  Revised date: 2022-01-13

  Online published: 2023-01-06

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摘要

该文采用四级杆-轨道阱液质联用仪(ultra high performance liquid chromatography quadrupole-orbitrap,UHPLC Q-Orbitrap)建立了同时检测玉米中4种主要真菌毒素黄曲霉毒素B1(aflatoxin B1,AFB1)、伏马菌素B1(fumonisin B1,FB1)、玉米赤霉烯酮(zearalenone,ZEA)和赭曲霉毒素A(ochratoxins A,OTA)的方法。首先对提取方式、提取时间和提取溶剂进行优化,同时研究玉米基质对4种真菌毒素的基质干扰效应以及低温高速离心的净化效果,然后进行方法验证,并将该方法与QuEChERS前处理方法进行比较。结果表明,低温高速离心能有效去除玉米基质对AFB1的信号抑制,对4种真菌毒素均能达到较好的定量效果;添加低浓度和高浓度时的加标回收率为90%~105%,相对标准偏差为0.7%~2.2%。该方法具有良好的准确度和精密度,4种真菌毒素线性相关系数r2>0.99,定量限为0.42~314 μg/kg。此方法能达到与QuEChERS一致的检测效果,并且对含有FB1样品的真菌毒素同时检测具有明显优势,是一种经济、高效的谷物中多种毒素分析方法。

关键词： 玉米; 真菌毒素; UHPLC Q-Orbitrap; 基质效应; QuEChERS

本文引用格式

吴红涛 , 李萌萌 , 关二旗 , 刘远晓 , 王瑞虎 , 卞科 . 四极杆-轨道阱液质联用法同时检测玉米中四种真菌毒素[J]. 食品与发酵工业, 2022 , 48(24) : 245 -251 . DOI: 10.13995/j.cnki.11-1802/ts.030338

Abstract

A method for simultaneous determination of aflatoxin B1 (AFB1), fumonisin B1 (FB1), zearalenone (ZEA), and ochratoxin A (OTA) in corn was established by ultra high performance liquid chromatography quadrupole-Orbitrap (UHPLC Q-Orbitrap). Firstly, the extraction method, extraction time, and extraction solvent were optimized, and the matrix effect of corn substrate on four kinds of mycotoxins and the purification effect of low temperature and high-speed centrifugation was studied, and then the method was verified. This method was compared with QuEChERS pretreatment methods. Results showed that low-temperature and high-speed centrifugation could effectively remove the signal inhibition of corn matrix to AFB1, and could achieve good quantitative results for four kinds of mycotoxins. The recovery rate of adding low concentration and high concentration was between 90% and 105%, and the relative standard deviation was 0.7% and 2.2%. The method had good accuracy and precision. The linear correlation coefficient of the four mycotoxins was R2>0.99. The quantitative limit was in the range of 0.42-314 μg/kg. This method can achieve the same detection effect as QuEChERS and has obvious advantages for the simultaneous detection of mycotoxins containing FB1. It is an economical and efficient method for the analysis of multiple toxins in grains.

Key words： maize; mycotoxins; UHPLC Q-Orbitrap; matrix effect; QuEChERS

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