综述与专题评论

鱼骨胶原蛋白提取、纯化工艺及应用的研究进展

  • 宫萱 ,
  • 包建强 ,
  • 黄可承 ,
  • 陈彦婕 ,
  • 唐嘉诚
展开
  • (上海海洋大学 食品学院,上海,201306)
第一作者:硕士研究生(包建强教授为通信作者,E-mail:baojq@shou.edu.cn)

收稿日期: 2021-10-08

  修回日期: 2021-11-30

  网络出版日期: 2023-01-06

基金资助

水产动物遗传育种中心上海市协同创新中心(ZF1206);上海市科委工程中心建设(11DZ2280300)

Progress on extraction, purification, and application of fish bone collagen

  • GONG Xuan ,
  • BAO Jianqiang ,
  • HUANG Kecheng ,
  • CHEN Yanjie ,
  • TANG Jiacheng
Expand
  • (College of Food Science, Shanghai Ocean University, Shanghai 201306, China)

Received date: 2021-10-08

  Revised date: 2021-11-30

  Online published: 2023-01-06

摘要

大多胶原蛋白是从哺乳动物的皮、骨、肉等部分获得的,但由于宗教信仰、疾病传播和各种过敏反应问题的出现,陆生源胶原蛋白在使用上受到了很大的限制。该文阐述了从水产品副产品——鱼骨中提取胶原蛋白的方法,并总结了3种常用的纯化鱼骨胶原蛋白的方法。此外,该综述还根据胶原蛋白的生理活性阐述了其在食品、化工品及医疗领域的研究进展及应用,为鱼骨胶原蛋白领域的探索提供了参考。

关键词: 鱼骨; 胶原蛋白; 方法; 应用

本文引用格式

宫萱 , 包建强 , 黄可承 , 陈彦婕 , 唐嘉诚 . 鱼骨胶原蛋白提取、纯化工艺及应用的研究进展[J]. 食品与发酵工业, 2022 , 48(24) : 346 -351 . DOI: 10.13995/j.cnki.11-1802/ts.029471

Abstract

Collagen is mostly obtained from mammalian skin, bone and meat. However, due to religious beliefs, disease transmission and various allergic reactions, the use of terrestrial collagen is greatly limited. In this review, the methods of extracting collagen from fish bone, a waste of aquatic products, were described, and three common methods of purifying fish bone collagen were summarized. In addition, this review also expounded on the research progress and application of collagen in the fields of food, chemicals and medical treatment according to its physiological activity, which could provide a reference for scholars in this field.

参考文献

[1] 赵海军, 王紫娟, 李政, 等.2020年我国进口水产品情况分析及对策研究[J].食品安全质量检测学报, 2021, 12(18):7 440-7 445.
ZHAO H J, WANG Z J, LI Z, et al.Analysis and countermeasures on imported aquatic products of China in 2020[J].Journal of Food Safety & Quality, 2021, 12(18):7 440-7 445.
[2] 袁美兰, 赵利, 刘华, 等.鱼头鱼骨的综合利用研究进展[J].现代农业科技, 2015, 656(18):284-286;288.
YUAN M L, ZHAO L, LIU H, et al.Comprehensive utilization of fish head and fish bone[J].Modern Agricultural Science and Technology, 2015, 656(18):284-286;288.
[3] 周广运, 樊雨梅, 刘楚怡, 等.驴骨胶原蛋白肽对骨质疏松大鼠的改善作用[J].食品工业科技, 2021, 42(22):336-343.
ZHOU G Y, FAN Y M, LIU C Y, et al.Effect of donkey bone collagen peptide on improving osteoporosis in rats[J].Science and Technology of Food Industry, 2021, 42(22):336-343.
[4] 杨华锋. 胶原蛋白贴敷料在皮肤美容中的临床应用研究[J].中国继续医学教育, 2018, 10(21):69-71.
YANG H F.Study on the Clinical application of collagen dressing in skin cosmetology[J].China Continuing Medical Education, 2018, 10(21):69-71.
[5] 李萍, 高利忠, 蓝岚秀子, 等.PTICE 预防幽门结扎大鼠胃溃疡及其作用机制[J].中国现代药物应用, 2008, 2(19):1-3.
LI P, GAO L Z, LAN L X Z, et al.Prevention and mechanism of pufferfish type I collagen extract for ulceration in pylorus ligature rats[J].Chinese Journal of Modern Drug Application, 2008, 2(19):1-3.
[6] NAGAI T, SUZUKI N.Isolation of collagen from fish waste material—skin, bone and fins[J].Food Chemistry, 2000, 68(3):277-281.
[7] 梁飞, 左红梅.胶原和水解胶原蛋白的结构、性能和功效研究及应用前景[J].明胶科学与技术, 2013, 33(3):115-123.
LIANG F, ZUO H M.A review of collagen and collagen hydrolysates:Structure, properties, functions, applications and prospect[J].The Science and Technology of Gelatin, 2013, 33(3):115-123.
[8] RANGEL U J S, ODA H, AKERMAN J, et al.Topical antibiotic elution in a collagen-rich hydrogel for healing of infected wounds[J].Plastic and Reconstructive Surgery-Global Open, 2020, 8(9S):141.
[9] 李军. 鲢鱼骨胶原多肽的制备及其抗氧化、钙螯合活性的研究[D].南昌:江西师范大学, 2020.
LI J.Study on the preparation and antioxidant, calcium chelating activities of collagen polypeptides from silver carp bone[D].Nanchang:Jiangxi Normal University, 2020.
[10] AHMED M, VERMA A K, PATEL R.Collagen extraction and recent biological activities of collagen peptides derived from sea-food waste:A review[J].Sustainable Chemistry and Pharmacy, 2020, 18:100315.
[11] 于浩. 鹿骨胶原蛋白的制备及抗氧化活性研究[D].长春:长春工业大学, 2016.
YU H.Preparation and antioxidant of collagen from deer bone[D].Changchun:Changchun University of Technology, 2016.
[12] 李妍花. 鸡爪皮胶原蛋白提取、自组装和消化特性研究[D].镇江:江苏大学, 2017.
LI Y H.Behavior of self-assembled and simulated digestion of isolated collagen from the chicken feet skin[D].Zhenjiang:Jiangsu University, 2017.
[13] 姚行行, 郭妍, 庄永亮.云南鲷鱼骨胶原蛋白的制备及其理化性质[J].食品科学, 2018, 39(13):35-40.
YAO X X, GUO Y, ZHUANG Y L.Preparation and physicochemical properties of collagen from bone of Yunnan bream[J].Food Science, 2018, 39(13):35-40.
[14] 冯建慧, 吴晓洒, 蔡路昀, 等.鲢鱼鱼皮和鱼骨胶原蛋白的提取及理化性质分析[J].中国食品学报, 2017, 17(7):102-108.
FENG J H, WU X S, CAI L Y, et al.Extraction and characterization of collagens from skin and bone of silver carp[J].Journal of Chinese Institute of Food Science and Technology, 2017, 17(7):102-108.
[15] 严秋萍, 邬应龙, 李月娥, 等.齐口裂腹鱼骨胶原蛋白超声波辅助提取工艺及其特性研究[J].食品与机械, 2017, 33(12):154-159.
YAN Q P, WU Y L, LI Y E, et al.Ultrasonic-assisted extraction process and characteristics analysis of collagen from Schizothorax prenanti bone[J].Food & Machinery, 2017, 33(12):154-159.
[16] 张延华, 马国红, 宋理平, 等.梭鱼骨胶原蛋白的提取及其性质[J].广东海洋大学学报, 2015, 35(4):104-108.
ZHANG Y H, MA G H, SONG L P, et al.Study on extraction and characteristics of the collagen from Liza haematocheila bone[J].Journal of Guangdong Ocean University, 2015, 35(4):104-108.
[17] 贾伟.牛骨营养品质评价与牦牛骨胶原蛋白肽功效研究[D]. 兰州:甘肃农业大学, 2017.
JIA W. The study of bovine bone nutritional quality assessment and yak (Bos grunniens) bone collagen peptide function[D]. Lanzhou: Gansu Agricultural University, 2017.
[18] 何云, 赵淑静, 汪有先, 等.鮟鱇鱼骨胶原蛋白提取工艺[J].食品工业科技, 2018, 39(5):185-190.
HE Y, ZHAO S J, WANG Y X, et al.Extraction technology of collagen in Lophius litulon bone[J].Science and Technology of Food Industry, 2018, 39(5):185-190.
[19] 张百刚,梁海荣,冯再平,等.龟甲胶原蛋白的提取工艺研究[J].江西农业学报, 2020, 32(5):83-87.
ZHANG B G, LIANG H R, FENG Z P, et al. Study on extraction technology of collagen from tortoise shell[J]. Acta Agriculturae Jiangxi, 2020, 32(5):83-87.
[20] 周婷,吴瑀婕,卢方云,等.鸡膝软骨胶原蛋白肽的超声辅助酶提工艺优化及抗氧化性[J].肉类研究, 2021, 35(4):7-15.
ZHOU T,WU Y J, LU F Y, et al. Optimization of ultrasound-assisted enzymatic extraction of collagen peptide from chicken knee cartilage and its antioxidant activity[J]. Meat Research, 2021, 35(4):7-15.
[21] 于淑池, 赖卓慧.金鲳鱼骨明胶的提取工艺及性质研究[J].食品研究与开发, 2020, 41(3):159-165.
YU S C, LAI Z H.Study on extraction technology and properties of gelatin from golden pompano bones[J].Food Research and Development, 2020, 41(3):159-165.
[22] 李美娜, 黎德佳, 谢景, 等.鲤鱼鱼鳞蛋白的酶解制备工艺及其抗氧化活性[J].安徽农业科学, 2017, 45(19):86-89.
LI M N, LI D J, XIE J, et al.Study on enzymatic hydrolysis preparation and antioxidant activity of protein from carp scales[J].Journal of Anhui Agricultural Sciences, 2017, 45(19):86-89.
[23] JEEVITHAN E, WU W H, WANG N P, et al.Isolation, purification and characterization of pepsin soluble collagen isolated from silvertip shark (Carcharhinus albimarginatus) skeletal and head bone[J].Process Biochemistry, 2014, 49(10):1 767-1 777.
[24] WANG Z B, WANG L, LIN S M, et al.Isolation and characterization of collagen from the muscle of Amur sturgeon (Acipenser schrenckii)[J].Biotechnology and Bioprocess Engineering, 2014, 19(5):935-941.
[25] 王正云, 蒋慧亮.超声波辅助酶法提取青鱼鱼皮胶原蛋白工艺[J].食品研究与开发, 2020, 41(5):133-137.
WANG Z Y, JIANG H L.Technology of ultrasound-assisted extraction of collagen from black carp skin[J].Food Research and Development, 2020, 41(5):133-137.
[26] 吴缇, 陈舜胜.斑点叉尾(鱼回)鱼骨制取胶原蛋白和骨粉的试验研究[J].南方水产, 2009, 5(3):36-40.
WU T, CHEN S S.Study on the extraction of collagen from channel catfish bone and bone meal[J].South China Fisheries Science, 2009, 5(3):36-40.
[27] NAGAI T, SUZUKI N.Isolation of collagen from fish waste material—skin, bone and fins[J].Food Chemistry, 2000, 68(3):277-281.
[28] 陈申如, 蔡扬鹏, 周琼, 等.鱼骨胶原蛋白的纯化及其特性的初步研究[J].食品科学, 2006, 27(11):177-181.
CHEN S R, CAI Y P, ZHOU Q, et al.Study on separation and purification collagens from fish bone[J].Food Science, 2006, 27(11):177-181.
[29] LI Z R, WANG B, CHI C F, et al.Isolation and characterization of acid soluble collagens and pepsin soluble collagens from the skin and bone of Spanish mackerel (Scomberomorous niphonius)[J].Food Hydrocolloids, 2013, 31(1):103-113.
[30] YU D, CHI C F, WANG B, et al.Characterization of acid-and pepsin-soluble collagens from spines and skulls of skipjack tuna(Katsuwonus pelamis)[J].Chinese Journal of Natural Medicines, 2014, 12(9):712-720.
[31] AHMED R, HAQ M, CHUN B S.Characterization of marine derived collagen extracted from the by-products of bigeye tuna (Thunnus obesus)[J].International Journal of Biological Macromolecules, 2019, 135:668-676.
[32] CUMMING M H, HALL B, HOFMAN K.Isolation and characterisation of major and minor collagens from hyaline cartilage of hoki (Macruronus novaezelandiae)[J].Marine Drugs, 2019, 17(4):223.
[33] DEYL Z, MIKŠÍK I, ECKHARDT A.Preparative procedures and purity assessment of collagen proteins[J].Journal of Chromatography B, 2003, 790(1-2):245-275.
[34] BURGESON, R E, HEBDA P A, MORRIS N P, et al.Human cartilage collagens.Comparison of cartilage collagens with human type V collagen[J].Journal of Biological Chemistry,1982, 257(13):7 852-7 856.
[35] 刘磊. 海蜇胶原蛋白的提取、纯化及理化性质研究[D].无锡:江南大学, 2009.
LIU L.Study on extraction and purification and the physical-chemical property of collagen from Rhopilema esculentum[D].Wuxi:Jiangnan University, 2009.
[36] MIURA S.Jellyfish mesogloea collagen[J].Journal of Biological Chemistry, 1985, 260:15 352-15 356.
[37] 陈思谨. 海洋红鼓鱼鱼鳞中Ⅰ型胶原蛋白的高效制备技术及其结构研究[D].南京:南京中医药大学, 2019.
CHEN S J.Studies on high efficient preparation technology and its structure of type I collagen from scales of red drum fish(Sciaenops ocellatus)[D].Nanjing:Nanjing University of Traditional Chinese Medicine, 2019.
[38] 张雄, 李云雁, 张凯, 等.酸溶性三文鱼皮胶原蛋白超滤脱盐的研究[J].食品工业科技, 2014, 35(21):69-72;76.
ZHANG X, LI Y Y, ZHANG K, et al.Application of ultrafiltration in the desalination process of acid-soluble collagen from salmon skin[J].Science and Technology of Food Industry, 2014, 35(21):69-72;76.
[39] 赵玉红, 孔保华, 张立钢.酶解鲢鱼副产物中蛋白质制备含肽运动饮料[J].食品工业, 2003, 24(2):24-25.
ZHAO Y H, KONG B H, ZHANG L G.Preparation of peptide containing sports beverage from protein of silver carp by-product by enzymatic hydrolysis[J].The Food Industry, 2003, 24(2):24-25.
[40] 杨志荣. 羊软骨中胶原蛋白肽的提取及其抑菌活性的研究[D].呼和浩特:内蒙古农业大学, 2016.
YANG Z R.Studies on extraction of collagen peptide from sheep cartilage and antibacterial activity of collagen peptide[D].Hohhot:Inner Mongolia Agricultural University, 2016.
[41] DUAN R, ZHANG J J, DU X Q, et al.Properties of collagen from skin, scale and bone of carp (Cyprinus carpio)[J].Food Chemistry, 2009, 112(3):702-706.
[42] 甘钊生, 梁志.鱼鳞胶原蛋白可食性复合涂膜对圣女果保鲜效果研究[J].农产品加工, 2019(16):17-19.
GAN Z S, LIANG Z.Study on the preservation effects of fish collagen edible coating on cherry tomatoes[J].Farm Products Processing, 2019(16):17-19.
[43] 沈徽霞. 一种哺乳母羊用酸化饲料的制备方法:中国, CN108902484A[P].2018-11-30.
SHEN H X.A preparation method of acidified feed for lactating ewes:China, CN108902484A[P].2018-11-30.
[44] SOUSA R O, ALVES A L, CARVALHO D N, et al.Acid and enzymatic extraction of collagen from Atlantic cod (Gadus Morhua) swim bladders envisaging health-related applications[J].Journal of Biomaterials Science. Polymer Edition, 2020, 31(1):20-37.
[45] 吴钢. 水产动物源胶原蛋白在医用敷料方向应用的研究进展[J].渔业研究, 2021, 43(4):436-442.
WU G.Advances and prospects in the research of aquatic collagen for medical dressing[J].Journal of Fisheries Research, 2021, 43(4):436-442.
[46] JENKINS D D, YANG G P, LORENZ H P, et al.Tissue engineering and regenerative medicine[J].Clinics in Plastic Surgery, 2003,30(4):581-588.
[47] CAO H,CHEN M M,LIU Y,et al.Fish collagen-based scaffold containing PLGA microspheres for controlled growth factor delivery in skin tissue engineering.[J].Colloids and Surfaces.B, Biointerfaces,2015,136:1 098-1 106.
[48] BUZNYK O, PASYECHNIKOVA N, ISLAM M M, et al.Bioengineered corneas grafted as alternatives to human donor corneas in three high-risk patients[J].Clinical & Translational Science, 2015, 8(5):558-562.
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