食品与发酵工业 >

2025 , Vol. 51 >Issue 3: 97 - 103

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.038913

研究报告

两株芽孢杆菌脂肽粗提物对马铃薯致病疫霉抑菌效果的研究

  • 黄满 ,
  • 李焕梅 ,
  • 胡靖 ,
  • 陈智勇 ,
  • 危俊杰 ,
  • 龙同 ,
  • 宋满 ,
  • 赵锦芳
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  • 1(湖北工业大学 生命科学与健康工程学院, 发酵工程教育部重点实验室, 湖北 武汉, 430068)
    2(湖北省生物农药工程技术研究中心, 湖北 武汉, 430064)
第一作者:硕士研究生(赵锦芳副教授为通信作者,E-mail:jinfangzhao@126.com)

收稿日期: 2024-02-20

  修回日期: 2024-04-10

  网络出版日期: 2025-02-21

基金资助

湖北省重点研发计划乡村振兴项目(2022BBA125);恩施州科技支撑项目(D20230080)

收起

Study on the antibacterial effect of two Bacillus lipopeptide crude extracts on potato Phytophthora infestans

  • HUANG Man ,
  • LI Huanmei ,
  • HU Jing ,
  • CHEN Zhiyong ,
  • WEI Junjie ,
  • LONG Tong ,
  • SONG Man ,
  • ZHAO Jinfang
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  • 1(School of Life Sciences and Health, Hubei University of Technology, Key Laboratory of Fermentation Engineering (Ministry of Education), Wuhan 430068, China)
    2(Hubei Biopesticide Engineering Research Center, Wuhan 430064, China)

Received date: 2024-02-20

  Revised date: 2024-04-10

  Online published: 2025-02-21

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摘要

为了探究多粘类芽孢杆菌Paenibacillus polymyxa HL11和卡氏芽孢杆菌Bacillus cabrialesii ST-1的脂肽粗提物对马铃薯致病疫霉的抑菌效果,该研究通过酸沉淀法提取多粘类芽孢杆菌HL11和卡氏芽孢杆菌ST-1的脂肽粗提物,利用牛津杯法测定两株芽孢杆菌的脂肽粗提物对致病疫霉菌Phytophthora infesfans的抑菌效果。结果表明,当脂肽粗提物处理质量浓度为10 mg/mL时,HL11和ST-1对致病疫霉的抑制率分别为(74.79±0.52)%和(79.5±0.54)%,通过软件拟合得出两株芽孢杆菌半最大效应浓度分别为0.56 mg/mL和0.021 mg/mL。进一步利用扫描电镜观察到经脂肽粗提物处理后,致病疫霉菌丝表面出现凹陷变形或突起等现象。使用荧光显微镜观察发现,经脂肽粗提物处理后,致病疫霉菌丝结构完整性受到一定程度的破坏,细胞内的染色质出现凝聚及活性氧激增现象。经基质辅助激光解吸电离飞行时间质谱分析和数据比对,脂肽类物质主要有伊枯草菌素A、表面活性素和丰原素。该研究中两株芽孢杆菌脂肽粗提物对马铃薯致病疫霉有明显的抑制作用,具有进一步研究开发潜力。

关键词： 多粘类芽孢杆菌HL11; 卡氏芽孢杆菌ST-1; 脂肽粗提物; 致病疫霉; 抑菌率

本文引用格式

黄满 , 李焕梅 , 胡靖 , 陈智勇 , 危俊杰 , 龙同 , 宋满 , 赵锦芳 . 两株芽孢杆菌脂肽粗提物对马铃薯致病疫霉抑菌效果的研究[J]. 食品与发酵工业, 2025 , 51(3) : 97 -103 . DOI: 10.13995/j.cnki.11-1802/ts.038913

Abstract

To investigate the bacteriostatic effect of crude lipopeptide extracts of Paenibacillus polymyxa HL11 and Bacillus cabrialesii ST-1 on Phytophthora infestans, the lipopeptide crude extracts of HL11 and ST-1 were extracted by acid precipitation method, and the antibacterial effect of the lipopeptide crude extracts of the two Bacillus strains on Phytophthora infesfans was determined by Oxford cup method.The results showed that the inhibition rates of HL11 and ST-1 against P.infestans were (74.79 ±0.52)% and (79.5±0.54)%, respectively, when the concentration of lipopeptide crude extract was 10 mg/mL. The EC50of the two strains of Bacillus were 0.56 and 0.021 mg/mL, respectively, as derived from the software fitting.Further observation by scanning electron microscopy showed that after treatment with lipopeptide crude extract, the surface of P.infestans showed depression, deformation or protrusions.Fluorescence microscopy showed that the structural integrity of P.infestans was destroyed to a certain extent after treatment with lipopeptide crude extract, and chromatin condensation and ROS surge occurred in the cells.By MALDI-TOF-MS analysis and data comparison, lipopeptides were mainly Iturin A, Surfactin and Fengycin.In this study, the crude lipopeptide extracts of two Bacillus strains had obvious inhibitory effect on Phytophthora infestans, and had the potential for further research and development.

Key words： Paenibacillus polymyxa HL11; Bacillus cabrialesii ST-1; lipopeptides; Phytophthora infestans; inhibitory rates

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