食品与发酵工业 >

2025 , Vol. 51 >Issue 3: 250 - 256

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.038723

研究报告

宣木瓜抗炎活性与成分

  • 周勤纯 ,
  • 徐德平
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  • (江南大学 食品学院, 江苏 无锡, 214000)
第一作者:硕士研究生(徐德平副教授为通信作者,E-mail:xdp1219@sina.com)

收稿日期: 2024-01-26

  修回日期: 2024-04-09

  网络出版日期: 2025-02-21

基金资助

中央高校基本科研业务费专项资金资助项目(JUSRP21127)

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Anti-inflammatory activity and ingredients of Chaenomeles

  • ZHOU Qinchun ,
  • XU Deping
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  • (School of Food Science and Technology, Jiangnan University, Wuxi 214000, China)

Received date: 2024-01-26

  Revised date: 2024-04-09

  Online published: 2025-02-21

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摘要

探究宣木瓜抗炎活性成分及其化学结构。以宣木瓜为原料,进行醇提、水提醇沉得到不同提取物,利用二甲苯诱导的小鼠耳肿胀模型测定其抗炎活性。醇提物经MCI层析柱、SephadexLX-20层析柱、ODS-AQ柱分离得到一个活性组分N,水提醇沉物经DEAE-纤维素柱、HW-55F凝胶柱和SephacryI S-400分离得到活性多糖PCC-1,采用小鼠耳肿胀模型结合小鼠血清炎症因子水平对抗炎活性进一步评价。结果表明,组分N和多糖PCC-1均有抗炎活性,并确定多糖PCC-1是主要功效成分,组分N只是辅助。通过核磁共振鉴定,组分N为二聚体原花青素,首次从木瓜中分离得到;多糖PCC-1由葡萄糖和葡萄糖醛酸组成,主链以β1→4键相连,主链上的C3,C2位有侧链相连,一部分C6位形成糖醛酸,一部分C6位被甲酯化。该多糖的糖醛酸结构在抗炎机制中发挥着关键作用。

关键词： 宣木瓜; 原花青素; 多糖; 抗炎; 分离鉴定

本文引用格式

周勤纯 , 徐德平 . 宣木瓜抗炎活性与成分[J]. 食品与发酵工业, 2025 , 51(3) : 250 -256 . DOI: 10.13995/j.cnki.11-1802/ts.038723

Abstract

The purpose of this study was to explore the anti-inflammatory active ingredients of Chaenomeles and their chemical structure.Ethanol extraction, water extraction and ethanol precipitation were used to obtain different extracts of Chaenomeles.To investigate the anti-inflammatory capacity, the xylene-induced mouse ear swelling test was conducted.Using column chromatography to fractionate and purify the extracts, two active compounds were isolated from different extracts.Compound N was separated by MCI, Sephadex LX-20 and ODS-AQ columns from the ethanol extraction.Polysaccharide PCC-1 was separated by DEAE-cellulose column, HW-55F gel column and SephacryI S-400 column from the water-extracted alcohol precipitate.The anti-inflammatory capacity of these two pure compounds was further evaluated using an ear swelling model.And the degree of swelling and inflammatory factors in serum were used as evaluation indicators.The result demonstrated that component N and polysaccharide PCC-1 are anti-inflammatory active compounds of Chaenomeles.Polysaccharide PCC-1 played a key role in anti-inflammation, and component N was only an auxiliary.According to the results of NMR spectrum analysis, component N was a dimer proanthocyanidin firstly separated from Chaneomeles. Polysaccharide PCC-1 was composed of glucose and glucuronic acid.The main chain of the polysaccharide was connected by β1→4 bonds.The C3 and C2 positions on the main chain were connected by side chains, part of the C6 site was formed into glucuronic acid, and part of the C6 site was methylated.The glucuronic acid structure of the polysaccharide played a key role in the anti-inflammatory mechanism.

Key words： Chaenomeles; proanthocyanidins; polysaccharide; anti-inflammatory; isolation and identification

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