食品与发酵工业 >

2025 , Vol. 51 >Issue 3: 257 - 264

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.038404

研究报告

草酸处理对采后银条褐变及能量代谢的影响

  • 李佩艳 ,
  • 苏娇 ,
  • 肖鑫鑫 ,
  • 罗登林 ,
  • 韩四海
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  • 1(河南科技大学 食品与生物工程学院, 河南 洛阳, 471023)
    2(河南省食品原料工程技术研究中心, 河南 洛阳, 471023)
    3(食品加工与安全国家级实验教学示范中心, 河南 洛阳, 471023)
第一作者:博士,副教授(通信作者,E-mail:lipeiyan77@163.com)

收稿日期: 2023-12-28

  修回日期: 2024-03-19

  网络出版日期: 2025-02-21

基金资助

国家自然科学基金项目(31701665);河南省重大公益专项(201300110300)

收起

Effects of oxalic acid treatment on browning and energy metabolism of postharvest Stachys flordana Schuttl.ex Benth

  • LI Peiyan ,
  • SU Jiao ,
  • XIAO Xinxin ,
  • LUO Denglin ,
  • HAN Sihai
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  • 1(College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China)
    2(Henan Engineering Research Center of Food Material, Luoyang 471023, China)
    3(National Experimental Teaching Demonstration Center of Food Processing and Security, Luoyang 471023, China)

Received date: 2023-12-28

  Revised date: 2024-03-19

  Online published: 2025-02-21

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摘要

为研究草酸处理对采后银条褐变的抑制作用,并阐明银条褐变及能量代谢之间的关系,该试验采用10 mmol/L草酸溶液处理银条20 min,之后10 ℃下贮藏30 d,测定银条褐变及能量代谢相关指标,分析草酸处理对银条褐变的影响,并研究其与银条能量代谢之间的关系。结果表明,与对照相比,草酸处理抑制了采后银条褐变指数(browning index,BI)的上升及L*值的下降,且在贮藏15~30 d差异显著;草酸处理显著降低了银条呼吸作用的增加,抑制了丙二醛(malondialdehyde,MDA)和相对电导率的上升,较好保持了银条细胞膜完整性,降低了银条多酚氧化酶(polyphenol oxidase,PPO)、过氧化物酶(peroxidase,POD)、苯丙氨酸解氨酶(phenylalanine aminolyase,PAL)活性以及总酚含量,有效减轻了银条酶促褐变发生;同时,草酸处理还提高了银条琥珀酸脱氢酶(succinate dehydrogenase,SDH)、细胞色素氧化酶(cytochrome oxidase,CCO)、H+-ATPase和Ca2+-ATPase活性,抑制了银条ATP、ADP及能荷(energy charge,EC)水平下降,维持了银条贮藏后期较高能量水平,从而延缓其褐变发生。相关性分析表明,银条BI与ATP、ADP、EC、H+-ATPase和Ca2+-ATPase呈极显著负相关,而与总酚、PPO、POD、PAL、AMP呈极显著正相关。综上,草酸处理抑制银条褐变的发生与提高能量代谢而维持银条较高能量水平有关,同时也与降低银条总酚含量、抑制褐变相关酶活性有关。

关键词： 草酸; 银条; 褐变; 能量代谢

本文引用格式

李佩艳 , 苏娇 , 肖鑫鑫 , 罗登林 , 韩四海 . 草酸处理对采后银条褐变及能量代谢的影响[J]. 食品与发酵工业, 2025 , 51(3) : 257 -264 . DOI: 10.13995/j.cnki.11-1802/ts.038404

Abstract

To investigate the control effect of oxalic acid treatment on browning of postharvest Stachys flordana Schuttl.ex Benth and to explore the relationship between browning and energy metabolism, Stachys flordana Schuttl.ex Benth was dipped in 10 mmol/L oxalic acid solution for 20 min, and then stored at 10 ℃ for 30 days.The related indexes of browning and energy metabolism of Stachys flordana Schuttl.ex Benth were measured, which was used to analyze the effect of oxalic acid treatment on browning and reveal the relationship between browning and energy metabolism.Results showed that, compared with the control, oxalic acid treatment could effectively inhibit the increase of the browning index and the decrease of the L* value, which showed a significant difference during 15-30 days of storage.Oxalic acid treatment remarkably reduced the increase of respiration, inhibited the increase of malondialdehyde (MDA) and relative conductivity, and better maintained the integrity of the cell membrane of Stachys flordana Schuttl.ex Benth.The activities of polyphenol oxidase (PPO), peroxidase (POD), and phenylalanine aminolyase (PAL) were reduced, and total phenolic content was also significantly decreased, which could reduce the browning of Stachys flordana Schuttl.ex Benth.Meanwhile, oxalic acid treatment could improve the activities of succinate dehydrogenase (SDH), cytochrome oxidase (CCO), H+-ATPase, and Ca2+-ATPase, inhibit the decrease of ATP, ADP, and energy charge (EC), and maintain a high energy level in late storage of Stachys flordana Schuttl.ex Benth, which could delay the browning of Stachys flordana Schuttl.ex Benth.Correlation analysis showed that the browning index of Stachys flordana Schuttl.ex Benth was negatively correlated with ATP, ADP, EC, H+-ATPase, and Ca2+-ATPase, while positively correlated with total phenolic, PPO, POD, PAL, and AMP content.In conclusion, the inhibition of oxalic acid treatment on the browning of Stachys flordana Schuttl.ex Benth was related to the improvement of energy metabolism and energy levels, as well as the reduction of total phenolic content and the activity of enzymes related to browning.

Key words： oxalic acid; Stachys flordana Schuttl.ex Benth; browning; energy metabolism

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