食品与发酵工业 >

2025 , Vol. 51 >Issue 3: 343 - 348

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.037914

分析与检测

柱前衍生-高效液相色谱法测定特殊医学用途奶粉中牛磺酸含量

  • 李政 ,
  • 张若煜 ,
  • 梅英杰 ,
  • 闫娅楠 ,
  • 刘阳 ,
  • 牟丹 ,
  • 安迈瑞 ,
  • 崔梦琪 ,
  • 张贞理
展开
  • (淄博市检验检测计量研究总院, 山东 淄博, 255000)
第一作者:硕士,助理工程师(张贞理高级工程师为通信作者,E-mail:zzl-1971@163.com)

收稿日期: 2023-11-10

  修回日期: 2023-12-13

  网络出版日期: 2025-02-21

基金资助

2021年度山东省市场监督管理局科研项目

收起

Determination of taurine content in formula powder for special medical by high performance liquid chromatography with pre-column derivatization

  • LI Zheng ,
  • ZHANG Ruoyu ,
  • MEI Yingjie ,
  • YAN Yanan ,
  • LIU Yang ,
  • MU Dan ,
  • AN Mairui ,
  • CUI Mengqi ,
  • ZHANG Zhenli
Expand
  • (Zibo Municipal General Institute of Inspection and Metrology, Zibo 255000, China)

Received date: 2023-11-10

  Revised date: 2023-12-13

  Online published: 2025-02-21

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摘要

建立以邻苯二甲醛为衍生试剂,柱前衍生-HPLC测定特殊医学用途奶粉中牛磺酸含量的方法。选用C18色谱柱进行产物分离,并对衍生试剂用量、流动相比例、流速、混合次数、衍生后等待时间等因素进行优化。结果表明衍生试剂用量30 μL,流动相体积比20∶80,流速0.6 mL/min,混合6次,衍生完成后等待时间0 min时,衍生物的峰面积和峰形最优。在此实验条件下3种不同浓度牛磺酸标准品的精密度测定衍生物峰面积相对标准偏差(relative standard deviation,RSD)为0.08%~0.75%,精密度高。对两种特殊医学用途奶粉中牛磺酸含量进行检测,重复性实验测定RSD分别为0.67%和0.37%,重复性稳定;样品加标平均回收率分别为97.6%~104.5%和106.0%~110.3%,回收率好。该研究方法反应条件温和、操作性强,适用于特殊医学用途奶粉中牛磺酸含量的测定。

关键词： 特殊医学用途奶粉; 牛磺酸; 柱前衍生; 高效液相色谱; 定量分析

本文引用格式

李政 , 张若煜 , 梅英杰 , 闫娅楠 , 刘阳 , 牟丹 , 安迈瑞 , 崔梦琪 , 张贞理 . 柱前衍生-高效液相色谱法测定特殊医学用途奶粉中牛磺酸含量[J]. 食品与发酵工业, 2025 , 51(3) : 343 -348 . DOI: 10.13995/j.cnki.11-1802/ts.037914

Abstract

A method for the determination of taurine content in formula powder for special medical by HPLC with ortho phthalaldehyde (OPA) as a pre-column derivatization reagent was established.The resulting extract was separated on a C18 column, and the addition amount of derivatization reagent, flow phase ratio, flow rate, mixing times, and waiting time after derivatization were optimized.The results of the tests showed that the peak area and pattern were optimal when the volume of the derivatization reagent dosage was 30 μL, the flow phase volume ratio was 20∶80, the flow rate was 0.6 mL/min, the mixing times was 6 times, and the waiting time after derivatization was 0 min.Under the experimental conditions, the relative standard deviation (RSD) of the peak area of the three different concentrations of taurine standards was 0.08%-0.75% and the precision was high.The content of taurine in two kinds of formula powder for special medical was detected and the RSD of the repeatability experiments were 0.67% and 0.37%, the repeatability was good.The test results of the spiked recovery were nice with the average recoveries of the samples were 97.6%-104.5% and 106.0%-110.3%, respectively.Therefore, the results showed that this analysis method had the advantages of mild reaction conditions and easy preparation for the determination of taurine content in formula powder for special medical.

Key words： formula powder for special medical; taurine; pre-column derivatization; HPLC; quantitative analysis

参考文献

[1] 陈丽丽, 白春清, 袁美兰, 等.高效液相色谱法测定鱼露中章鱼胺和牛磺酸[J].食品与发酵工业, 2020, 46(2):261-265.
CHEN L L, BAI C Q, YUAN M L, et al.Determination of octopamine and taurine in fish sauce by HPLC[J].Food and Fermentation Industries, 2020, 46(2):261-265.
[2] ALI OMER M M, ALI OMAR M M, ABDELAZIZ M A, et al.Liquid chromatographic and spectrophotometric determination of taurine in energy drinks based on O-phthalaldehyde-sulfite derivatization[J].Journal of Food Chemistry & Nanotechnology, 2019, 5(1):1-7.
[3] 谢昭鹏, 姚昱锟, 刘斌雄, 等.牛磺酸提取及检测方法的研究进展[J].食品工业科技, 2019, 40(24):323-331.
XIE Z P, YAO Y K, LIU B X, et al.Research progress on extraction and detection methods of taurine[J].Science and Technology of Food Industry, 2019, 40(24):323-331.
[4] 徐彬, 李飞, 赵海萍, 等.柱前衍生法测定功能性饮料与口服液中的牛磺酸[J].食品研究与开发, 2019, 40(10):145-151.
XU B, LI F, ZHAO H P, et al.Determination of taurine in energy drinks and oral liquids by pre-column derivatization[J].Food Research and Development, 2019, 40(10):145-151.
[5] 卞华, 翁史昱, 王亮.基于二维液相色谱体系分析特殊医学用途奶粉中的牛磺酸含量[J].中国食品学报, 2022, 22(3):288-296.
BIAN H, WENG S Y, WANG L.Analysis on the taurine in the milk powder with special medical purpose based on the two-dimensional liquid chromatography system[J].Journal of Chinese Institute of Food Science and Technology, 2022, 22(3):288-296.
[6] MEHDINIA A, ROSTAMI S, DADKHAH S, et al.Simultaneous screening of homotaurine and taurine in marine macro-algae using liquid chromatography-fluorescence detection[J].Journal of the Iranian Chemical Society, 2017, 14(10):2135-2142.
[7] GILL B D, WOOD J E.Rapid analysis of taurine in infant formula and adult nutritionals by hydrophilic interaction liquid chromatography-mass spectrometry[J].Journal of AOAC International, 2023, 106(2):384-388.
[8] 张婷, 陈雷, 刘芸, 等.高效液相色谱-高分辨质谱法测定乳粉中的牛磺酸[J].食品安全质量检测学报, 2017, 8(6):2086-2090.
ZHANG T, CHEN L, LIU Y, et al.Determination of taurine in milk powder by high performance liquid chromatography-high resolution mass spectrometry[J].Journal of Food Safety & Quality, 2017, 8(6):2086-2090.
[9] 严俊, 徐柏杨, 颜琳琦, 等.L-8900氨基酸分析仪检测不同形态保健食品中牛磺酸含量[J].食品工业, 2019, 40(11):341-344.
YAN J, XU B Y, YAN L Q, et al.Determination of taurine in different forms health food by L-8900 amino acid analyzer[J].The Food Industry, 2019, 40(11):341-344.
[10] MOHAMED S, ELBASHIR A, ABOUL-ENEIN H.Spectrophotometric method for determination of taurine in energy drinks[J].Current Pharmaceutical Analysis, 2016, 13(1):11-17.
[11] GONZÁLEZ-VÁZQUEZ M, MEZA-MÁRQUEZ O G, GALLARDO-VELÁZQUEZ T, et al.Simultaneous determination of caffeine and taurine in energy drinks by FT-MIR spectroscopy coupled with multivariate analysis[J].Journal of Spectroscopy, 2020, 2020:8835846.
[12] LEE I, VO J, GAO Q Y, et al.Single-laboratory validation study of a proton NMR method for the determination of L-arginine, L-citrulline, and taurine contents in dietary supplements[J].Journal of AOAC International, 2020, 103(4):1140-1147.
[13] 吴海智, 肖玥惠子, 朱礼, 等.程序进样-柱前衍生-高效液相色谱法测定乳制品中牛磺酸含量[J].食品研究与开发, 2021, 42(21):116-120.
WU H Z, XIAO Y, ZHU L, et al.Determination of taurine content in dairy products using program sampling-precolumn derivatization-high performance liquid chromatography[J].Food Research and Development, 2021, 42(21):116-120.
[14] 黄丽丹, 邢亚东, 王玲玲, 等.2, 4-二硝基氟苯柱前衍生化HPLC法测定保健食品中牛磺酸含量[J].药物分析杂志, 2016, 36(1):171-175.
HUANG L D, XING Y D, WANG L L, et al.Determination of taurine in health food by RP-HPLC with pre-column derivatization[J].Chinese Journal of Pharmaceutical Analysis, 2016, 36(1):171-175.
[15] 赵超敏, 蒋颖婕, 曾静, 等.高效液相色谱法测定乳粉及液态奶中牛磺酸含量[J].现代食品科技, 2019, 35(1):252-256;205.
ZHAO C M, JIANG Y J, ZENG J, et al.Determination of taurine content in milk powder and liquid milk by high performance liquid chromatography[J].Modern Food Science and Technology, 2019, 35(1):252-256;205.
[16] 姚程晨, 杨丽梅, 林立.特殊医学用途婴儿配方食品中牛磺酸含量的测定[J].中国乳品工业, 2022, 50(12):44-48.
YAO C C, YANG L M, LIN L.Detection of taurine in formulas for special medical purposes intended for infants[J].China Dairy Industry, 2022, 50(12):44-48.
[17] 李宇, 张乃合, 刘洪海.牛磺酸分析方法的研究进展[J].中国卫生检验杂志, 2021, 31(17):2167-2173.
LI Y, ZHANG N H, LIU H H.Research progress on analytical methods of taurine[J].Chinese Journal of Health Laboratory Technology, 2021, 31(17):2167-2173.
[18] 柳小英, 毛丽沙, 鲍敏洁, 等.高效液相色谱法测定海洋生物中牛磺酸的含量[J].安徽农业科学, 2017, 45(31):1-3;11.
LIU X Y, MAO L S, BAO M J, et al.Detection of taurine in marine life by high performance liquid chromatography[J].Journal of Anhui Agricultural Sciences, 2017, 45(31):1-3;11.
[19] 秦梦然, 张伟, 吴凤明, 等.高效液相色谱法检测5种市售鸡蛋中牛磺酸含量[J].食品研究与开发, 2022, 43(1):167-172.
QIN M R, ZHANG W, WU F M, et al.Determination of taurine content in five brands of commercially available eggs by high performance liquid chromatography[J].Food Research and Development, 2022, 43(1):167-172.
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