食品与发酵工业 >

2025 , Vol. 51 >Issue 4: 123 - 130

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.038975

研究报告

基于宏基因组学分析不同膳食结构非酒精性脂肪肝患者的肠道菌群特点

  • 肖瑞 ,
  • 郭建锋 ,
  • 王琳琳 ,
  • 田培郡 ,
  • 金星 ,
  • 赵建新 ,
  • 陈卫 ,
  • 王刚
展开
  • 1(江南大学 食品学院,江苏 无锡,214122)
    2(宜兴市人民医院,江苏 无锡,214200)
第一作者:硕士研究生(王刚教授为通信作者,E-mail:wanggang@jiangnan.edu.cn)

收稿日期: 2024-02-26

  修回日期: 2024-04-12

  网络出版日期: 2025-03-10

基金资助

国家自然科学基金面上项目(32272332)

收起

Metagenomic analysis reveals dietary structure-dependent gut microbiota characteristics in non-alcoholic fatty liver disease patients

  • XIAO Rui ,
  • GUO Jianfeng ,
  • WANG Linlin ,
  • TIAN Peijun ,
  • JIN Xing ,
  • ZHAO Jianxin ,
  • CHEN Wei ,
  • WANG Gang
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  • 1(School of Food Science and Technology, Jiangnan University, Wuxi 214122, China)
    2(Yixing People's Hospital, Wuxi 214200, China)

Received date: 2024-02-26

  Revised date: 2024-04-12

  Online published: 2025-03-10

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摘要

非酒精性脂肪肝病是世界范围内的主要代谢类疾病之一,其与肠道菌群联系紧密。由于大量关于非酒精性脂肪肝病肠道标志物研究结果的不均一性,因此需要考虑是否是饮食差异所导致的。该研究纳入30名不同饮食结构的非酒精性脂肪肝病患者,分为15名高脂肪膳食摄入患者和15名高碳水膳食摄入患者,基于宏基因组学测序方法研究患者的肠道菌群。结果发现,2种膳食模式下的非酒精性脂肪肝病患者肠道菌群存在显著差异,在种水平上,长期高碳水摄入的患者肠道菌群以梭菌为主,长期高水平脂肪摄入者的肠道优势菌群则主要是普拉梭杆菌(Feacalibacterium prausnitzii);在功能上,高脂肪摄入患者富集在胆酸代谢通路上,而高碳水摄入患者富集在棕榈酸酯合成通路上。这一研究对未来的非酒精性脂肪肝动物特异性造模和进一步科学合理地指导非酒精性脂肪肝患者合理膳食有一定意义。

关键词： 宏基因组; 非酒精性脂肪肝; 肠道菌群; 膳食结构

本文引用格式

肖瑞 , 郭建锋 , 王琳琳 , 田培郡 , 金星 , 赵建新 , 陈卫 , 王刚 . 基于宏基因组学分析不同膳食结构非酒精性脂肪肝患者的肠道菌群特点[J]. 食品与发酵工业, 2025 , 51(4) : 123 -130 . DOI: 10.13995/j.cnki.11-1802/ts.038975

Abstract

Non-alcoholic fatty liver disease (NAFLD) stands as a prevalent metabolic disorder globally, with its pathogenesis intricately linked to the gut microbiome.Given the diverse outcomes of numerous studies on gut microbial markers in NAFLD, the impact of dietary variations warrants examination.This investigation incorporated thirty NAFLD patients with distinct dietary patterns, categorizing them into two groups:fifteen individuals consuming a high-fat diet and another fifteen on a high-carbohydrate regimen.Employing metagenomic sequencing, this study analyzed the gut microbiota composition of these cohorts.Notably, significant discrepancies were observed in the microbial communities between the two dietary models.Specifically, individuals with prolonged high carbohydrate consumption predominantly harbored Clostridium species, whereas those with a sustained high-fat intake were characterized by an abundance of Faecalibacterium prausnitzii. From a functional standpoint, the microbiota of high-fat consumers showed enrichment in the bile acid metabolic pathway, whereas those on high-carbohydrate diets demonstrated a predominance in the palmitic acid synthesis pathway.These findings underscore the critical influence of dietary structure on gut microbiota composition in NAFLD patients and highlight the potential for targeted dietary interventions to modulate microbial pathways relevant to NAFLD pathogenesis.The insights gleaned from this study pave the way for tailored animal models of NAFLD and advocate for scientifically grounded dietary recommendations for individuals with this condition.

Key words： metagenomics; non-alcoholic fatty liver disease; gut microbiota; dietary structure

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