泻剂结肠是一种伴有严重肠神经和肠屏障损伤的泻药依赖性便秘,益生菌干预有望成为其有效的治疗手段。该文应用番泻叶提取物处理构建了泻剂结肠动物模型,研究副干酪乳酪杆菌CCFM1164对泻剂结肠的缓解作用,并探究其可能的作用途径。结果表明,副干酪乳酪杆菌CCFM1164能缩短泻剂结肠小鼠排首粒黑便时间及增加5 h内粪便粒数以缓解便秘,其缓解泻剂结肠的可能途径是通过增加乳杆菌属(Lactobacillus)产短链脂肪酸(short chain fatty acids, SCFAs)菌的相对丰度而提高粪便中SCFAs含量,增加结肠乙酰胆碱的含量进而促进胶质细胞源性神经营养因子的分泌以达到修复肠神经系统的效果,进一步降低肠道炎症,修复肠道机械屏障。该研究为益生菌缓解泻剂结肠提供了理论基础。
Cathartic colon is a type of laxative-dependent constipation accompanied by severe enteric nerve and intestinal barrier damage, and probiotic intervention is expected to be an effective treatment for it.In this study, an animal model of the cathartic colon was established using senna extract treatment to study the alleviating effect of Lacticaseibacillus paracasei CCFM1164 on the cathartic colon and to investigate its possible pathways.Results showed that L. paracasei CCFM1164 shortened the time to pass the first black stool and increased the number of fecal pellets within 5 h to relieve constipation in cathartic colon mice.The possible mechanisms underlying the alleviation of the cathartic colon were increasing the relative abundance of short-chain fatty acids (SCFAs)-producing bacteria, such as Lactobacillus and Marvinbryantia, to increase the content of SCFAs in the feces, enhancing the content of acetylcholine (ACh) in the colon, which in turn promotes the secretion of glial cell line-derived neurotrophic factor (GDNF) and thereby repairs the enteric nervous system, and further reducing intestinal inflammation and repairing the intestinal mechanical barrier.This study provides a theoretical basis for probiotics to relieve the cathartic colon.
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