食品与发酵工业 >

2025 , Vol. 51 >Issue 5: 119 - 125

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.039005

研究报告

发酵豆粕对结肠炎小鼠的缓解效果及机制

  • 刘静宇 ,
  • 翟波 ,
  • 杨小雁 ,
  • 房舒琪 ,
  • 毛银 ,
  • 邓禹
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  • 1(江南大学,粮食发酵与食品生物制造国家工程研究中心,江苏 无锡,214122)
    2(江南大学 生物工程学院,江苏 无锡,214122)
    3(江苏省生物活性制品加工工程技术研究中心,江苏 无锡,214122)
    4(山东渤海实业集团有限公司,山东省油脂油料精深加工技术重点实验室,山东 滨州,256500)
第一作者:硕士研究生(毛银实验师和邓禹教授为共同通信作者,E-mail:maoymaoy@163.com;dengyu@jiangnan.edu.cn)

收稿日期: 2024-02-28

  修回日期: 2024-03-27

  网络出版日期: 2025-03-28

基金资助

山东省重点研发项目(2022CXPT042);广西壮族自治区重点研发计划项目(桂科AB22035063)

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Effectiveness and mechanism of fermented soybean meal in alleviating colitis in mice

  • LIU Jingyu ,
  • ZHAI Bo ,
  • YANG Xiaoyan ,
  • FANG Shuqi ,
  • MAO Yin ,
  • DENG Yu
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  • 1(National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Wuxi 214122, China)
    2(School of Biotechnology, Jiangnan University, Wuxi 214122, China)
    3(Jiangsu Bioactive Products Processing Engineering Technology Research Center, Wuxi 214122, China)
    4(Shandong Key Laboratory of Oil and Oil Deep Processing Technology, Shandong Bohai Industrial Group Company Ltd., Binzhou 256500, China)

Received date: 2024-02-28

  Revised date: 2024-03-27

  Online published: 2025-03-28

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摘要

该研究旨在探究发酵豆粕对小鼠结肠炎的缓解效果及可能机制,寻找一种有效替抗的生物饲料。采用葡聚糖硫酸钠诱导小鼠溃疡性结肠炎模型,以植物乳杆菌DY6发酵豆粕进行饲喂治疗,考察发酵豆粕的抗炎和修复肠道黏膜作用。结果显示,与模型组相比,发酵饲料提前干预和治疗都能够显著提高小鼠进食量、体重,有效降低死亡率、小鼠疾病活动指数评分,有效促进结肠结构糜烂出血和萎缩等情况的恢复,明显缓解促炎因子白细胞介素-6和肿瘤坏死因子α含量上升的情况,显著增加小鼠结肠组织的紧密连接蛋白中闭合蛋白的表达水平。说明发酵豆粕能够抑制炎症发生的信号通路,减轻体内炎症反应,有效保护肠道黏膜完整性,达到缓解结肠炎的效果。该研究为养殖业防治动物结肠炎提供理论基础,有望开发一种功能性饲料。

关键词： 发酵豆粕; 饲料; 植物乳杆菌; 葡聚糖硫酸钠; 结肠炎

本文引用格式

刘静宇 , 翟波 , 杨小雁 , 房舒琪 , 毛银 , 邓禹 . 发酵豆粕对结肠炎小鼠的缓解效果及机制[J]. 食品与发酵工业, 2025 , 51(5) : 119 -125 . DOI: 10.13995/j.cnki.11-1802/ts.039005

Abstract

To explore the alleviating effects and potential mechanisms of fermented soybean meal on colitis in mice, and seek an effective antibiotic alternative bio-feed, a dextran sulfate sodium-induced model of ulcerative colitis was employed, with treatment using fermented soybean meal by Lactobacillus plantarum DY6, to investigate the anti-inflammatory and intestinal mucosal repair capabilities.The results indicated that, both early intervention and treatment with the fermented feed significantly increased food intake and body weight of the mice, effectively reduced the mortality rate, and lowered the disease activity index scores compared to the model group.Moreover, it effectively facilitated the recovery of colonic structure from erosion, bleeding, and atrophy, significantly alleviated the increase in pro-inflammatory cytokines IL-6 and TNF-α levels, and markedly increased the expression level of closure protein in the tight junction of mouse colon tissue.The results suggested that fermented soybean meal can inhibit the signaling pathways leading to inflammation, mitigate the inflammatory response, and effectively protect the integrity of the intestinal mucosa, thereby alleviating colitis.This study provides theories and ideas for the prevention and control of animals colitis in the aquaculture industry, and is expected to develop a functional feed.

Key words： fermented soybean meal; feed; Lactobacillus plantarum; dextran sodium sulfate; colitis

参考文献

[1] 吴莲云. 壳寡糖对动物实验性炎性肠病防治作用的研究[D].湛江:广东海洋大学, 2020.
WU L Y.Study on the prevention and treatment of chitooligosaccharide on animal experimental inflammatory bowel disease[D].Zhanjiang:GuangDong Ocean University, 2020.
[2] SUN M Y, LIU Y J, SONG Y L, et al.The ameliorative effect of Lactobacillus plantarum-12 on DSS-induced murine colitis[J].Food & Function, 2020, 11(6):5205-5222.
[3] YU P, KE C X, GUO J X, et al.Lactobacillus plantarum L15 alleviates colitis by inhibiting LPS-mediated NF-κB activation and ameliorates DSS-induced gut microbiota dysbiosis[J].Frontiers in Immunology, 2020, 11:575173.
[4] LI X Q, ZHU Y H, ZHANG H F, et al.Risks associated with high-dose Lactobacillus rhamnosus in an Escherichia coli model of piglet diarrhoea:Intestinal microbiota and immune imbalances[J].PLoS One, 2012, 7(7):e40666.
[5] 王文文. 发酵豆粕对断奶仔猪肠道屏障的保护作用及机理研究[D].呼和浩特:内蒙古农业大学, 2023.
WANG W W.Protective effect and mechanism of fermented soybean meal on gut barrier in weaned piglets[D].Hohhot:Inner MongoliaAgricultural University, 2023.
[6] 余俊达. 一株短小芽孢杆菌用于豆粕固态发酵及防治细菌性腹泻的初步研究[D].长沙:湖南农业大学, 2020.
YU J D.Preliminary study on a strain of Bacillus pumilus used for solid fermentation of soybean meal and prevention of bacterial diarrhea[D].Changsha:Hunan Agricultural University, 2020.
[7] 陶红艳, 徐欣维, 汪明金, 等.乳酸菌益生特性及其应用的研究进展[J].安徽农学通报, 2021, 27(21):45-48.
TAO H Y, XU X W, WANG M J, et al.Research progress on probiotic characteristics and application of lactic acid bacteria[J].Anhui Agricultural Science Bulletin, 2021, 27(21):45-48.
[8] ZHU J J, GAO M X, ZHANG R L, et al.Effects of soybean meal fermented by L.plantarum, B.subtilis and S.cerevisieae on growth, immune function and intestinal morphology in weaned piglets[J].Microbial Cell Factories, 2017, 16(1):191.
[9] 鲁春灵, 李军国, 蒋万春, 等.湿态发酵豆粕对肉鸡生长性能、抗氧化能力和肠道健康的影响[J].动物营养学报, 2021, 33(7):4162-4174.
LU C L, LI J G, JIANG W C, et al.Effects of wet fermented soybean meal on growth performance, antioxidant capability and intestine healthy of broilers[J].Chinese Journal of Animal Nutrition, 2021, 33(7):4162-4174.
[10] CATALÁN N, VILLASANTE A, WACYK J, et al.Fermented soybean meal increases lactic acid bacteria in gut microbiota of atlantic salmon (Salmo salar)[J].Probiotics and Antimicrobial Proteins, 2018, 10(3):566-576.
[11] 刘颖颖, 卢艳波, 杨小雁, 等.植物乳杆菌DY6的生理特性及代谢机制分析[J].食品与发酵工业, 2022, 48(18):11-19.
LIU Y Y, LU Y B, YANG X Y, et al.Physiological characteristics of Lactobacillus plantarum DY6 and its metabolic mechanisms analysis[J].Food and Fermentation Industries, 2022, 48(18):11-19.
[12] 陆春波, 毛银, 李国辉, 等.植物乳杆菌DY6主要抑菌代谢物的分析和鉴定[J].微生物学通报, 2019, 46(9):2258-2271.
LU C B, MAO Y, LI G H, et al.Analysis and identification of main antibacterial metabolites secreted by Lactobacillus plantarum DY6[J].Microbiology China, 2019, 46(9):2258-2271.
[13] 毛银, 陆春波, 李国辉, 等.菌酶协同发酵豆粕工艺的优化[J].食品与发酵工业, 2019, 45(14):108-114.
MAO Y, LU C B, LI G H, et al.Optimized fermentation process of soybean meal by bacteria with enzymes[J].Food and Fermentation Industries, 2019, 45(14):108-114.
[14] 吴思琪. 植物乳杆菌Zhang-LL对DSS诱导大鼠慢性溃疡性结肠炎预防效果评价[D].北京:北京农学院, 2018.
WU S Q.Effect on DSS-induced chronic ulcerative colitis in rats of Lactobacillus Plantarum Zhang-LL[D].Beijing:Beijing University of Agriculture, 2018.
[15] 侯楠楠, 王梅, 王倩, 等.乳酸菌固态发酵豆粕产酸性能分析[J].中国酿造, 2022, 41(9):124-129.
HOU N N, WANG M, WANG Q, et al.Acid production from soybean meal by solid-state fermentation with lactic acid bacteria[J].China Brewing, 2022, 41(9):124-129.
[16] 梁静, 张文举, 王博.L-苹果酸的生理功能及其在反刍动物生产应用中的研究进展[J].中国畜牧兽医, 2016, 43(7):1916-1921.
LIANG J, ZHANG W J, WANG B.Research progress on the physiological function of L-malic acid and its application in ruminant production[J].China Animal Husbandry & Veterinary Medicine, 2016, 43(7):1916-1921.
[17] 杨紫嫣. 副干酪乳杆菌对DSS诱导小鼠结肠炎免疫调节作用的研究[D].湛江:广东医科大学, 2020.
YANG Z Y.Immunomodulatory effect of Lactobacillus paracasei on DSS-induced colitis in mice[D].Zhanjiang:Guangdong Medical University, 2020.
[18] 薛永强, 张辉华, 王达, 等.短链脂肪酸对肠道健康的调控机制及在动物生产中的应用[J].饲料工业, 2020, 41(19):18-22.
XUE Y Q, ZHANG H H, WANG D, et al.Regulation mechanism of short-chain fatty acids on intestinal health and their application in animal production[J].Feed Industry, 2020, 41(19):18-22.
[19] CHEN M D, LIU Y Y, XIONG S B, et al.Dietary L-tryptophan alleviated LPS-induced intestinal barrier injury by regulating tight junctions in a Caco-2 cell monolayer model[J].Food & Function, 2019, 10(5):2390-2398.
[20] 向大育. 发酵豆粕对断奶仔猪肠道形态及消化酶活性的影响[J].中国饲料, 2019(17):48-50;54.
XIANG D Y.Effect of fermented soybean meal on intestinal morphology and digestive enzyme activity of weaned piglets[J].China Feed, 2019(17):48-50;54.
[21] XU C H, LIU J H, GAO J W, et al.Combined soluble fiber-mediated intestinal microbiota improve insulin sensitivity of obese mice[J].Nutrients, 2020, 12(2):351.
[22] LI Y, DE HAAR C, CHEN M, et al.Disease-related expression of the IL6/STAT3/SOCS3 signalling pathway in ulcerative colitis and ulcerative colitis-related carcinogenesis[J].GUT, 2010, 59(2):227-235.
[23] XIA Y J, CHEN Y, WANG G Q, et al. Lactobacillus plantarum AR113 alleviates DSS-induced colitis by regulating the TLR4/MyD88/NF-κB pathway and gut microbiota composition[J].Journal of Functional Foods, 2020, 67:103854.
[24] 侯保秋, 董怡文, 刘方.一株分离自牦牛乳中的副干酪乳杆菌ZZ102对溃疡性结肠炎的缓解作用研究[J].中国乳品工业, 2023, 51(10):21-25.
HOU B Q, DONG Y W, LIU F.Alleviative effect on ulcerative colitis of Lactobacillus paracasei ZZ102 isolated from yak milk[J].China Dairy Industry, 2023, 51(10):21-25.
[25] RAVINDRAN R, LOEBBERMANN J, NAKAYA H I, et al.The amino acid sensor GCN2 controls gut inflammation by inhibiting inflammasome activation[J].Nature, 2016, 531(7595):523-527.
[26] LAN J, DOU X J, LI J W, et al.L-Arginine ameliorates lipopolysaccharide-induced intestinal inflammation through Inhibiting the TLR4/NF-κB and MAPK pathways and stimulating β-defensin expression in vivo and in vitro[J].Journal of Agricultural and Food Chemistry, 2020, 68(9):2648-2663.
[27] 左云洋, 胡萍, 许浩翔, 等.副干酪乳杆菌SR10-1发酵刺梨汁对葡聚糖硫酸钠诱导小鼠溃疡性结肠炎的缓解作用[J].食品科学, 2023, 44(15):121-128.
ZUO Y Y, HU P, XU H X, et al.Relieving effect of Rosa roxburghii tratt.Juice fermented by Lactobacillus paracasei SR10-1 on dextran sulfate sodium-Induced ulcerative colitis in mice[J].Food Science, 2023, 44(15):121-128.
[28] SRIVASTAVA A K, VENKATA B S, SWEAT Y Y, et al.Serine 408 phosphorylation is a molecular switch that regulates structure and function of the occludin a-helical bundle[J].Proceedings of the National Academy of Sciences of the United States of America, 2022, 119(34):e2204618119.
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