为优化无核白葡萄赤霉素(gibberellin acid,GA3)处理方案,提升葡萄及葡萄干品质,以无核白葡萄为试材,设置5种GA3处理(花期不处理+花后10 d 150 mg/L GA3、花序盛开75% 75 mg/L GA3 +花后10 d 150 mg/L GA3、花序盛开100% 75 mg/L GA3 +花后10 d 150 g/L GA3、花后2 d 75 g/L GA3 +花后10 d 150 g/L GA3、花后4 d 75 g/L GA3 +花后10 d 150 g/L GA3),以花期、花后10 d均不进行GA3处理为对照组,测定葡萄、葡萄干品质及产量指标。结果表明,GA3处理可有效增大无核白葡萄果穗、果粒质量及果形指数,提高鲜果硬度和维生素C含量,促进葡萄色泽变绿。经GA3处理的无核白葡萄所制葡萄干色泽 L*值、a*值降低,葡萄干亮度降低、色泽变绿;花后2 d 75 g/L GA3 +花后10 d 150 mg/L GA3处理的葡萄及葡萄干产量最高,分别达74.10 t/hm2和17.33 t/hm2,葡萄干单粒质量、果形指数最大,分别为1.20 g和2.96,具有较高的酚类物质和抗氧化活性。主成分分析结果直观地反映出各处理葡萄、葡萄干品质分布状况,建立综合评价模型得出花后2 d 75 mg/L GA3 +花后10 d 150 mg/LGA3处理综合得分最高,应用效果最好。
The goals of this study was to optimize gibberellin acid (GA3) treatment measure on Thompson Seedless, and to improve the quality of grapes and raisins.This study taken Thompson Seedless grape as material, the experiment consisted of five treatments no treatment during bloom +150 mg/L GA3, 10 days post-bloom, 75 mg/L GA3, 75% bloom +150 mg/L GA3, 10 days post-bloom, 75 mg/L GA3, 100% bloom +150 mg/L GA3, 10 days post-bloom, 75 mg/L GA3, 2 days post-bloom +150 mg/L GA3, 10 days post-bloom, 75 mg/L GA3, 4 days post-bloom +150 mg/L GA3, 10 days post-bloom, no spraying (bloom and post-bloom) was the control, berries quality, raisin quality, and yield were assessed.The results showed that cluster weight, berry weight, shape index, berry hardness and vitamin C content increased with GA3 treatment, promotes the color to green.The L* and a* value of raisin were decreased with GA3 treatment, raisins lost brightness and turned green.The yields of grape and raisin were the highest in (75 mg/L GA3, 2 days post-bloom +150 mg/L GA3, 10 days post-bloom) treatment, with 74.10 t/hm2and 17.33 t/hm2, respectively, single raisin weight and raisin shape index were also the highest in (75 mg/L GA3, 2 days post-bloom +150 mg/L GA3, 10 days post-bloom) treatment, 1.20 g and 2.96, respectively, phenolic substances and antioxidant activity were relatively high.The results of principal component analysis directly reflected the quality distribution of berries and raisins in each treatment, (75 mg/L GA3, 2 days post-bloom +150 mg/L GA3, 10 days post-bloom) had the highest comprehensive score by comprehensive evaluation model, and the effect on Thompson Seedless with (75 mg/L GA3, 2 days post-bloom + 150 mg/L GA3, 10 days post-bloom) was the best.
[1] 蔡翔宇, 张进财.吐鲁番葡萄及其加工业概况与优化提升策略[J].中外葡萄与葡萄酒, 2022(5):112-116.
CAI X Y, ZHANG J C.Present situation and optimization strategy of grape and its processing industry in Turpan[J].Sino-Overseas Grapevine & Wine, 2022(5):112-116.
[2] 户金鸽, 白世践.无核白葡萄营养系果实品质及葡萄干特性分析[J].新疆农业科学, 2023, 60(11):2751-2763.
HU J G, BAI S J.Study on the fruit quality and raisins character of Thompson seedless and its lines[J].Xinjiang Agricultural Sciences, 2023, 60(11):2751-2763.
[3] 杨丽丽, 牛早柱, 魏建国, 等.外源GA3对葡萄花序伸长及赤霉素合成代谢相关基因表达的影响[J].分子植物育种, 2020, 18(17):5600-5606.
YANG L L, NIU Z Z, WEI J G, et al.Effects of exogenous GA3 on inflorescence elongation and gibberellin anabolism related gene expression in grapevine[J].Molecular Plant Breeding, 2020, 18(17):5600-5606.
[4] REYNOLDS A, ROBBINS N, LEE H S, et al.Impacts and interactions of abscisic acid and gibberellic acid on sovereign coronation and skookum seedless table grapes[J].American Journal of Enology and Viticulture, 2016, 67(3):327-338.
[5] SUEHIRO Y, MOCHIDA K, TSUMA M, et al.Effects of gibberellic acid/cytokinin treatments on berry development and maturation in the yellow-green skinned ‘shine Muscat’ grape[J].The Horticulture Journal, 2019, 88(2):202-213.
[6] KOUKOURIKOU M, ZIOZIOU E, PANTAZAKI A, et al.Effects of gibberellic acid and putrescine on ‘Thompson seedless’ grapes[J].American International Journal of Biology, 2015, 3(2):19-29.
[7] 张瑜, 哈胜英.无核白葡萄不同生育期喷施赤霉素效果[J].农村科技, 2020(1):19-20.
ZHANG Y, HA S Y.Effects of spraying gibberellin on seedless white grapes at different growth stages[J].Rural Science & Technology, 2020(1):19-20.
[8] 谢辉, 张雯, 韩守安, 等.新疆晾房环境对绿色葡萄干色泽的影响[J].农业工程学报, 2019, 35(7):295-302.
XIE H, ZHANG W, HAN S A, et al.Effect of drying room environment on color of green raisin in Xinjiang[J].Transactions of the Chinese Society of Agricultural Engineering, 2019, 35(7):295-302.
[9] Organtisation Internationle de la Vigne et du Vin.Organtisation Internationle de la Vigne et du Vin Descriptor List for Grape Varieties and Vitis Species[M].Paris:Organisation internationale de la vigne etdu vin, 2007.
[10] 唐莎莎. 无核白葡萄果实品质评价研究[D].乌鲁木齐:新疆农业大学, 2013.
TANG S S.The research on evaluation of fruit quality of the Thompson Seedless grape[D].Urumqi:Xinjiang Agricultural University, 2013.
[11] 高俊凤. 植物生理学实验指导[M].北京:高等教育出版社, 2006:203-204.
GAO J F. Experimental Guidance for Plant Physiology[M]. Beijing: High Education Press, 2006: 203-204.
[12] MERCURIO M D, DAMBERGS R G, HERDERICH M J, et al.High throughput analysis of red wine and grape phenolics-adaptation and validation of methyl cellulose precipitable tannin assay and modified Somers color assay to a rapid 96 well plate format[J].Journal of Agricultural and Food Chemistry, 2007, 55(12):4651-4657.
[13] TIAN S F, WANG Y, DU G, et al.Changes in contents and antioxidant activity of phenolic compounds during gibberellin-induced development in Vitis vinifera L.‘Muscat’[J].Acta Physiologiae Plantarum, 2011, 33(6):2467-2475.
[14] LI Y G, TANNER G, LARKIN P.TheDMACA-HCl protocol and the threshold proanthocyanidin content for bloat safety in forage legumes[J].Journal of the Science of Food and Agriculture, 1996, 70(1):89-101.
[15] WOLFE K, WU X Z, LIU R H.Antioxidant activity of apple peels[J].Journal of Agricultural and Food Chemistry, 2003, 51(3):609-614.
[16] 张妍, 孙娟, 包菲菲, 等.大兴安岭地区蓝果忍冬果实多酚鉴定及生物活性分析[J].食品科学, 2023, 44(12):225-234.
ZHANG Y, SUN J, BAO F F, et al.Identification and bioactivity analysis of polyphenols in blue honeysuckle fruit from different regions of the greater khingan mountains[J].Food Science, 2023, 44(12):225-234.
[17] BENZIE I F F, STRAIN J J.The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”:The FRAP assay[J].Analytical Biochemistry, 1996, 239(1):70-76.
[18] CASANOVA L, CASANOVA R, MORET A, et al.The application of gibberellic acid increases berry size of “Emperatriz” seedless grape[J].Spanish Journal of Agricultural Research, 2009, 7(4):919-927.
[19] 白世践, 郑明, 户金鸽.不同植物生长调节剂对无核白葡萄及葡萄干产量和品质的影响[J].黑龙江农业科学, 2023(11):77-82.
BAI S J, ZHENG M, HU J G.Effects of different plant growth regulators on quality and yield of Thompson seedless grape and raisin[J].Heilongjiang Agricultural Sciences, 2023(11):77-82.
[20] 农慧兰, 董阳, 刘静, 等.GA3和TDZ处理对‘阳光玫瑰’葡萄果实形状的影响[J/OL].分子植物育种, 2022.DOI:46.1068.s.20220518.1119.004.
NONG H L, DONG Y, LIU J, et al.The effect of GA3 and TDZ treatment on fruit shape of ‘shine muscat’ grape[J/OL].Molecular Plant Breeding, 2022.DOI:46.1068.s.20220518.1119.004.
[21] 颜孙安, 姚清华, 林香信, 等.成熟度对红地球葡萄品质的影响[J].食品安全质量检测学报, 2020, 11(14):4581-4588.
YAN S A, YAO Q H, LIN X X, et al.Effects of maturity on quality of red globe grape (Vitis vinifera L.)[J].Journal of Food Safety & Quality, 2020, 11(14):4581-4588.
[22] 刘峰娟, 冯作山, 孟阳, 等.脱水速度对‘无核白’葡萄果皮褐变和细胞超微结构的影响[J].食品科学, 2016, 37(6):220-225.
LIU F J, FENG Z S, MENG Y, et al.Effect of dehydration rate on browning and cell ultrastructure of Thompson seedless grapes[J].Food Science, 2016, 37(6):220-225.
[23] 李晓丽, 陈计峦, 范盈盈, 等.无核白葡萄干制过程中酚类物质的变化及其与褐变的关系[J].食品科学, 2019, 40(7):27-32.
LI X L, CHEN J L, FAN Y Y, et al.Relationship between changes of phenolic substances and browning during drying of Thompson seedless grapes[J].Food Science, 2019, 40(7):27-32.
[24] KELEBEK H, JOURDES M, SELLI S, et al.Comparative evaluation of the phenolic content and antioxidant capacity of Sun-dried raisins[J].Journal of the Science of Food and Agriculture, 2013, 93(12):2963-2972.
[25] JADHAV U, MUNDHE S, KUMAR Y, et al.Gibberellic acid induces unique molecular responses in ‘Thompson seedless’ grapes as revealed by non-targeted metabolomics[J].Journal of Plant Growth Regulation, 2021, 40(1):293-304.
[26] ADAMS D O.Phenolics and ripening in grape berries[J].American Journal of Enology and Viticulture, 2006, 57(3):249-256.
[27] KAPŁAN M, KLIMEK K, JABŁOŃSKA-RYŚ E, et al.Effect of hormonization treatment on yield quantity and quality, contents of biologically active compounds, and antioxidant activity in ‘einset seedless’ grapevine fruits and raisins[J].Molecules, 2021, 26(20):6206.
[28] QIN L W, WANG H, ZHANG W, et al.Effects of different drying methods on phenolic substances and antioxidant activities of seedless raisins[J].LWT, 2020, 131:109807.