通过建立C57BL/6小鼠脱发模型研究了副干酪乳酪杆菌CCFM1349对毛发再生的影响。在动物实验中,连续21 d向小鼠灌胃副干酪乳酪杆菌CCFM1349活菌,分析其对脱发小鼠背部毛发生长状态,皮肤组织中毛囊数量的影响。采用定量聚合酶链反应测定了小鼠背部皮肤中Wnt/β-catenin通路和凋亡通路相关蛋白的表达水平,并通过ELISA试剂盒检测小鼠皮肤中促毛发生长因子和抑毛发生长因子的含量。结果表明,副干酪乳酪杆菌CCFM1349显著促进脱发小鼠的毛发生长,14 d时小鼠背部毛发覆盖率为76.64%。在分子水平上,副干酪乳酪杆菌CCFM1349干预后,Wnt/β-catenin通路中的Wnt5a mRNA,Wnt10b mRNA和LEF1 mRNA表达分别上调至2.09、4.67和5.53倍,抑凋亡因子Bcl2 mRNA表达显著上调至3.51倍,促凋亡因子BAX mRNA表达下调了16.69%。同时小鼠皮肤中促毛发生长因子VEGF和IGF-1含量有显著性提升,分别增加了10.60%和19.65%。抑毛发生长因子TGF-β1含量显著下调12.70%。结果表明,副干酪乳酪杆菌CCFM1349通过调控Wnt/β-catenin通路,抑制细胞凋亡以及促进生长因子的表达促进小鼠的毛发生长。
The effect of Lacticaseibacillus paracasei CCFM1349 on the prevention and regeneration of hair loss was investigated by establishing a C57BL/6 mice hair loss model.In the animal experiment, mice were administered L.paracasei CCFM1349 for 21 days, observing its effects on hair growth and the number of hair follicles in the skin tissue of alopecia mice.The expression levels of proteins related to the Wnt/β-catenin pathway and apoptosis pathway in the back skin of mice were determined using quantitative polymerase chain reaction, while ELISA was used to detect the levels of hair growth factor and hair growth inhibitory factor in mice skin.Results demonstrated that L.paracasei CCFM1349 significantly promoted hair growth in alopecia mice, with a 76.64% coverage rate observed at day 14.At the molecular level, after intervention with L.paracasei CCFM1349, mRNA expression levels for Wnt5a, Wnt10b, and LEF1 in the Wnt/β-catenin pathway were upregulated by factors of 2.09-fold, 4.67-fold, and 5.53-fold respectively.Furthermore, mRNA expression levels for anti-apoptosis factor Bcl2 were significantly upregulated by a factor of 3.51-fold while a factor of 16.69% downregulated mRNA expression levels for proapoptotic factor BAX.Simultaneously, there was a significant increase of VEGF (10.60%) and IGF-1(19.65%), both being important hair growth factors found within mice skin.The content of TGF-β1 was significantly decreased by 12.70%.Results indicated that L.paracasei CCFM1349 promote hair growth in mice by regulating the Wnt/β-catenin pathway, inhibiting apoptosis and promoting the expression of growth factors.
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