为深入挖掘羊栖菜速溶粉的高附加值,通过分析测定羊栖菜速溶粉的总酚、总黄酮含量以及DPPH自由基、羟自由基、氧自由基和ABTS阳离子自由基清除能力;进一步以秀丽隐杆线虫为模型,测定其对线虫寿命、繁殖力、运动能力、脂褐素、热应激、氧化应激、体内抗氧化酶活力和丙二醛、活性氧积累的影响,评价其抗氧化作用。结果表明,速溶粉的总酚和总黄酮含量分别为72.64 GAE mg/g和52.97 RE mg/g。速溶粉可显著呈剂量依赖性清除DPPH自由基、羟自由基、氧自由基和ABTS阳离子自由基。线虫实验结果表明,与空白对照组相比,速溶粉的低剂量组(0.5 mg/mL)、中剂量组(1.0 mg/mL)、高剂量组(1.5 mg/mL)能分别提高线虫的平均寿命6.7%、14.12%和18.87%。羊栖菜速溶粉能降低线虫体内脂褐素的积累和繁殖力,改善线虫的运动能力,并提高线虫在氧化应激和热应激氧化损伤状态下的修复能力。此外,羊栖菜速溶粉还能够显著提高线虫体内超氧化物歧化酶、谷胱甘肽和过氧化氢酶的活性,并降低丙二醛和活性氧的积累,表现出显著的抗氧化活性。
To further explore the high added value of Sargassum fusiforme instant powder, the total phenols and flavonoids contents, as well as and the of DPPH, hydroxyl, oxygen and ABTS free radical scavenging ability of S. fusiforme instant powder were analyzed and determined.Further, using the Caenorhabditis elegans as a model, the antioxidant effect of S. fusiforme instant powder was evaluated by measuring its effects on lifespan, reproductive capacity, exercise ability, lipofuscin, heat stress, oxidative stress, in vivo antioxidant enzyme activity and accumulation of malondialdehyde and reactive oxygen species of C.elegans.The results showed that the total phenols and flavonoids contents of instant powder were 72.64 (GAE mg/g) and 52.97 (RE mg/g), respectively.Instant powder could significantly scavenge DPPH, hydroxyl, oxygen and ABTS cation free radicals in a dose-dependent manner.The results of C.elegans experiments showed that compared with the blank control group, the low-dose group (0.5 mg/mL), medium-dose group (1.0 mg/mL) and high-dose group (1.5 mg/mL) of instant powder could increase the average lifespan of C.elegans by 6.7%, 14.12% and 18.87%, respectively.S. fusiforme instant powder could reduce the lipofuscin accumulation and reproductive capacity in C.elegans, improve the movement ability of C.elegans, and improve the repair ability of C.elegans under oxidative stress and heat stress oxidative damage.In addition, S. fusiforme instant powder can significantly increase the activities of superoxide dismutase, glutathione and catalase in C.elegans, and reduce the accumulation of malondialdehyde and reactive oxygen species, showing significant antioxidant activity.
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