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代谢工程改造谷氨酸棒杆菌产L-缬氨酸研究进展

  • 王梦蓉 ,
  • 黄明珠 ,
  • 刘斌 ,
  • 陈雪岚
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  • 1(江西师范大学 生命科学学院, 国家淡水鱼加工技术研发专业中心,江西 南昌,330027)
    2(江西师范大学 健康学院,江西 南昌,330027)
第一作者:硕士研究生(陈雪岚教授为通信作者,E-mail:xuelanchen162@163.com)

收稿日期: 2024-07-04

  修回日期: 2024-12-12

  网络出版日期: 2025-04-29

基金资助

国家自然科学基金项目(32260014, 31960014);江西省自然科学基金项目(20224BAB215004, 20224BAB215005)

Research progress on production of L-valine from Corynebacterium glutamicum modified by metabolic engineering

  • WANG Mengrong ,
  • HUANG Mingzhu ,
  • LIU Bin ,
  • CHEN Xuelan
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  • 1(College of Life Science, National R&D Center for Freshwater Fish Processing, Jiangxi Normal University, Nanchang 330027, China)
    2(Health College, Jiangxi Normal University, Nanchang 330027, China)

Received date: 2024-07-04

  Revised date: 2024-12-12

  Online published: 2025-04-29

摘要

L-缬氨酸作为一种人体必需的支链氨基酸,因其具有多种生理功能被广泛应用于食品、医药、饲料及化妆品等行业。目前,微生物代谢工程技术的进步及用其构建的工程菌发酵生产具备环保、高效、无污染的特点,使微生物发酵法成为生产L-缬氨酸的首选方法。该文主要阐述了谷氨酸棒杆菌中L-缬氨酸生物合成途径,并对构建L-缬氨酸高产菌株的代谢工程技术及策略进行了综述,以期为进一步提高L-缬氨酸产量提供一定的参考。

本文引用格式

王梦蓉 , 黄明珠 , 刘斌 , 陈雪岚 . 代谢工程改造谷氨酸棒杆菌产L-缬氨酸研究进展[J]. 食品与发酵工业, 2025 , 51(7) : 343 -350 . DOI: 10.13995/j.cnki.11-1802/ts.040390

Abstract

L-valine, an essential branched-chain amino acid, is widely utilized in the food, pharmaceutical, feed, and cosmetic industries owing to its diverse physiological functions.With the advancement of microbial metabolic engineering technology, the fermentation process of engineered bacteria has emerged as the preferred method for producing L-valine due to its high efficiency, lack of pollution, and environmental protection potential.This study reviewed the metabolic engineering methods and strategies for creating high-yielding strains of L-valine, as well as the L-valine biosynthesis pathway from Corynebacterium glutamicum.It also provided references for pertinent researchers looking to enhance L-valine production.

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