食品与发酵工业 >

2025 , Vol. 51 >Issue 11: 171 - 178

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.041962

研究报告

分离、鉴定和筛选具有益生特性和降胆固醇能力的发酵马乳源益生菌

  • 库尔旦·胡达依别尔根 ,
  • 刘学莲 ,
  • 卡吾萨尔·恰依马尔旦 ,
  • 何瑶 ,
  • 巴合班·叶尔江 ,
  • 新华·那比
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  • 1(新疆医科大学 药学院, 新疆 乌鲁木齐, 830017)
    2(新疆及中亚特色医药资源教育部工程研究中心, 新疆 乌鲁木齐, 830017)
第一作者:硕士研究生(新华·那比教授为通信作者,E-mail:xh2022@xjmu.edu.cn)

收稿日期: 2024-12-26

  修回日期: 2025-01-22

  网络出版日期: 2025-06-19

基金资助

新疆维吾尔自治区研究生科研创新项目(XJ2024G189);新疆维吾尔自治区重大科技专项(2022A02013-3)

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Isolation, identification, and screening of probiotic strains with cholesterol-lowering potential from fermented mare’s milk

  • KUERDAN Hudayibieergen ,
  • LIU Xuelian ,
  • KAWUSAER Qiayimaerdan ,
  • HE Yao ,
  • BAHEBAN Yeerjiang ,
  • XINHUA Nabi
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  • 1(School of Pharmacy, Xinjiang Medical University, Urumchi 830017, China)
    2(Engineering Research Center of Xinjiang and Central Asian Medicine Resources, Ministry of Education, Urumchi 830017, China)

Received date: 2024-12-26

  Revised date: 2025-01-22

  Online published: 2025-06-19

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摘要

为筛选具有益生特性和降胆固醇能力的发酵马乳源发酵菌。该研究从传统发酵马乳中分离纯化了4株乳酸菌,并对其形态学和生理生化特征,生长曲线、胆盐耐受能力、人工胃液耐受能力和抗生素敏感性等特性进行了评价。进而通过胆盐水解酶活性定性检测法和胆固醇氧化酶法评估了发酵菌在体外的胆固醇清除能力,最后对筛选的菌株进行了16S rRNA鉴定。结果表明,分离的4株菌对高胆盐(3.0 g/L)和人工胃液(pH 2.0)具有较好的耐受性并且对所选8种抗生素多数表现敏感;在4株菌的滤纸片周围观察到了明显的白色沉淀圈,表明其均具有胆盐水解酶活性;单一菌及复合菌对培养基中胆固醇的清除率为(76.46±1.26)%~(87.96±0.43)%,且复合菌胆固醇清除能力高于其单一菌株,最后经16S rRNA基因测序鉴定为植物乳植杆菌(Lactiplantibacillus plantarum)。可见分离的4株菌具有益生的基本特性和较高的安全性,同时在体外具有较好的降胆固醇能力,可进一步探究其在体内的机制。

关键词： 传统发酵马乳; 益生特性; 抗生素敏感性; 胆盐水解酶; 降胆固醇作用

本文引用格式

库尔旦·胡达依别尔根 , 刘学莲 , 卡吾萨尔·恰依马尔旦 , 何瑶 , 巴合班·叶尔江 , 新华·那比 . 分离、鉴定和筛选具有益生特性和降胆固醇能力的发酵马乳源益生菌[J]. 食品与发酵工业, 2025 , 51(11) : 171 -178 . DOI: 10.13995/j.cnki.11-1802/ts.041962

Abstract

This study investigates the probiotic properties and cholesterol-lowering abilities of fermentation bacteria, by isolating and purifying four strains of lactic acid bacteria from traditional fermented mare’s milk.The morphological, physiological, and biochemical characteristics of these strains were assessed, along with their growth curves, tolerance to bile salts and artificial gastric fluid, and susceptibility to antibiotics.Additionally, qualitative assays were conducted for bile salt hydrolase activity and cholesterol oxidase to evaluate their cholesterol scavenging potential.The strains were ultimately identified using 16S rRNA gene sequencing.The findings reveal that the isolated strains exhibited strong tolerance to high concentrations of bile salts (3.0 g/L) and artificial gastric fluid (pH 2.0) while remaining sensitive to the majority of the eight antibiotics tested.Distinct white precipitation zones around the filter paper disks of each strain indicated the presence of bile salt hydrolase activity.The cholesterol scavenging rates for single and composite strains in the culture medium ranged from (76.46±1.26)% to (87.96±0.43)%, with the composite strain demonstrating superior capacity for cholesterol compared to individual strains.These strains had been identified as Lactiplantibacillus plantarum.This study suggests that the four isolated strains possess fundamental probiotic attributes and a high safety profile, along with notable cholesterol-lowering capabilities in vitro, which warrant further explored in vivo.

Key words： traditional fermented mare’s milk; probiotic properties; antibiotic sensitivity; bile salt hydrolase; cholesterol-lowering effect

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