食品与发酵工业 >

2025 , Vol. 51 >Issue 12: 106 - 114

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.040334

研究报告

腾冲姜黄精油的成分及其对变异链球菌抑菌机理的初探

  • 杨晓娜 ,
  • 徐玲 ,
  • 李田 ,
  • 张翼飞 ,
  • 陈自宏
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  • (保山学院 资源环境学院,云南 保山,678000)
第一作者:硕士,副教授(陈自宏教授为通信作者,E-mail:498131662@qq.com)

收稿日期: 2024-07-01

  修回日期: 2024-08-22

  网络出版日期: 2025-07-11

基金资助

国家自然科学地区基金项目(32060638);云南省教育厅教师类项目(2023J1122);云南省中青年学术和技术带头人后备人才项目(202205AC160061);云南省教育厅高黎贡山虫生真菌资源保护与绿色发展重点实验室建设项目;保山学院科研项目(22BYZY02)

收起

Composition of Tengchong turmeric essential oil and preliminary study on its antibacterial mechanism against Streptococcus mutans

  • YANG Xiaona ,
  • XU Ling ,
  • LI Tian ,
  • ZHANG Yifei ,
  • CHEN Zihong
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  • (School of Resources and Environment, Baoshan University, Baoshan 678000, China)

Received date: 2024-07-01

  Revised date: 2024-08-22

  Online published: 2025-07-11

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摘要

考察腾冲姜黄精油对变异链球菌的抑菌活性,探讨其主要的活性成分及可能的作用机理。采用GC-MS技术,结合Kovats保留指数对其精油主要成分进行分析,用不同浓度精油处理变异链球菌,观察处理前后变异链球菌的微观形态、表面电荷、细胞膜完整性、电导率各层面变化的情况。结果表明,姜黄精油主要成分有α-姜黄烯、α-姜烯、β-红没药烯、β-倍半水芹烯、芳姜黄酮、姜黄酮和β-姜黄素。其精油对变异链球菌的最低抑菌浓度为0.25 mg/mL,最小杀菌浓度为0.50 mg/mL。在培养基中加入最低抑菌浓度的姜黄精油后,变异链球菌的生长被完全抑制,部分菌体细胞膜破裂,细胞内物质流出,表面电荷减少,部分变异链球菌细胞受损,电导率在0~4 h有较升高的趋势,在4~8 h升高较缓慢,而在培养基中加入最小杀菌浓度的姜黄精油后,变异链球菌生长已停滞,甚至杀死,细胞群粘连在一起,菌体的稳定性变差,所有菌体细胞膜都被破坏,细胞内部的离子被释放至菌体外部环境,电导率的显著提升,从而导致细胞死亡,与对照组相比,姜黄精油具有抑制变异链球菌的作用。研究结果为姜黄精油防治龋齿提供重要参考依据。

关键词： 姜黄精油; 变异链球菌; 抑菌活性; 抑菌机理

本文引用格式

杨晓娜 , 徐玲 , 李田 , 张翼飞 , 陈自宏 . 腾冲姜黄精油的成分及其对变异链球菌抑菌机理的初探[J]. 食品与发酵工业, 2025 , 51(12) : 106 -114 . DOI: 10.13995/j.cnki.11-1802/ts.040334

Abstract

The antibacterial activity of Tengchong turmeric essential oil against Streptococcus mutans was investigated to explore its main active components and potential mechanism of action.The major components of the essential oil were analyzed by gas chromatography-mass spectrometry combined with Kovats retention index.S.mutans was treated with different concentrations of essential oil.The changes in its microscopic morphology, surface charge, membrane integrity, and electrical conductivity were observed before and after treatment.Results showed that the major components of turmeric essential oil were α-curcumene, α-zingiberene, β-bisabolene, β-sesquiphellandrene, ar-turmerone, turmerone, and β-curcumin.The minimum inhibitory concentration (MIC) of essential oil against S.mutans was 0.25 mg/mL and the minimum bactericidal concentration (MBC) was 0.5 mg/mL.After adding turmeric essential oil with the minimum inhibitory concentration to the culture medium, the growth of S.mutans was completely inhibited.The cell membranes of some bacteria were ruptured, intracellular substances flowed out, the surface charge decreased, and some S.mutans cells were damaged.The electrical conductivity showed a relatively higher increasing trend at 0-4 hours and increased more slowly at 4-8 hours.However, after adding turmeric essential oi with the minimum bactericidal concentration to the culture medium, the growth of S.mutans had stagnated and even been killed.The cell clusters were adhered together, the stability of the bacteria became worse, all the cell membranes of the bacteria were destroyed, and the ions inside the cells were released to the external environment of the bacteria.The significant increase in electrical conductivity led to cell death.Compared with the control group, turmeric essential oil has the effect of inhibiting S.mutans.The research results provide an important reference basis for the prevention and treatment of dental caries with turmeric essential oil.

Key words： turmeric essential oil; Streptococcus mutans; antibacterial activity; antibacterial mechanism

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