便秘是一种常见的胃肠道疾病,目前用于治疗便秘的主要药物包括润滑性或刺激性泻药,但这些药物存在着价格昂贵、易产生药物依赖等问题,因此寻求一种药物替代品迫在眉睫。随着益生菌与人体健康的研究日渐受到全球关注,通过补充益生菌调节肠道菌群,最终实现便秘的缓解逐渐成为治疗便秘的重要手段。黑茶富含膳食纤维、多糖和维生素等功能性物质,具有促进胃肠道的蠕动、帮助消化、利尿通便等功效,被称为肠胃的“清洁工”。该研究利用黑茶和实验室保藏的植物乳植杆菌BC299以及商品化益生菌复配,制成2款益生菌复配黑茶(BC和KC),并利用BC299、商品化益生菌与黑茶进行发酵,制备了2款益生菌发酵黑茶(BFC和KFC)。分析了益生菌协同黑茶对盐酸洛哌丁胺诱导便秘小鼠的改善作用。结果显示,益生菌复配黑茶和益生菌发酵黑茶均能显著增加便秘小鼠粪便含水率和小肠转运率,缩短便秘小鼠的首粒黑便排出时间,并提高便秘小鼠血清中胃动素、胃泌素和P物质的含量。同时,益生菌协同黑茶能有效修复便秘引起的小肠组织损伤,显著降低肠道组织中炎症因子TNF-α、IL-1β的含量,增加抗炎因子IL-10的含量。其中,综合评估益生菌复配黑茶BC改善便秘的效果最好。16S rDNA测序结果表明,益生菌协同黑茶均能够改变肠道菌群的结构和组成,提高了便秘小鼠肠道中厚壁菌门/拟杆菌门的比例,尤其是提高了乳杆菌属的丰度。综上所述,益生菌协同黑茶能有效缓解盐酸洛哌丁胺诱导的小鼠便秘,修复肠屏障损伤,调节肠道菌群组成和结构。该研究为拓展益生菌的研究方向、开发新型干预和缓解便秘的功能性食品提供了新的研究思路和重要参考。
Constipation, a prevalent gastrointestinal ailment, primarily responds to medications like lubricating or stimulant laxatives;however, these treatments are often costly and accompanied by a risk of dependency.Consequently, there is a pressing demand for a viable alternative.Amidst the growing global focus on probiotics and their impact on human health, supplementing with probiotics has emerged as a significant therapeutic approach for constipation, aiming to harmonize intestinal flora and ultimately alleviate symptoms.Moreover, dark tea, renowned as the “purifier” of the digestive system, boasts an abundance of functional components like dietary fiber, polysaccharides, and vitamins.These elements collectively contribute to enhancing gastrointestinal motility, facilitating digestion, promoting urination, and acting as a natural laxative.Thus, dark tea presents itself as a promising adjunct in the quest for effective constipation management. In this study, two probiotic-infused dark teas, designated as BC and KC, were meticulously crafted by blending dark tea with laboratory-cultured Lactobacillus plantarum BC299 and commercially available probiotic formulations.Additionally, two distinct probiotic-fermented dark teas, BFC and KFC, were produced using dark tea through a fermentation process by BC299 or commercial probiotics.The primary objective of this investigation was to delve into the synergistic effects of dark tea, both in its original form and when combined with probiotics, on alleviating constipation induced by loperamide hydrochloride in mice models.By analyzing these formulations, this study aimed to uncover potential therapeutic benefits and further the understanding of the interplay between traditional teas and modern probiotics in constipation.The findings revealed that the integration of probiotic dark tea and probiotic-fermented dark tea exhibited marked efficacy in enhancing the fecal water content and elevating the small intestinal transit rate among constipated mice.Furthermore, it significantly shortened the time to the first black stool expulsion in these animals.Notably, the combination also augmented the serum levels of motilin (MTL), gastrin (GAS), and tachykinin substance P (SP) in constipated mice, indicating a positive modulation of gut motility and digestive hormone profiles.Concurrently, the fusion of probiotics and dark tea effectively addressed the harm inflicted on small intestinal tissue by constipation, prominently diminishing the concentration of inflammatory cytokines TNF-α and IL-1β within the intestinal tissue.Moreover, this combination promoted a favorable inflammatory balance by augmenting the levels of the anti-inflammatory factor IL-10.Among the various formulations tested, the comprehensive assessment revealed that the probiotics-infused dark tea BC demonstrated the most pronounced efficacy in ameliorating constipation.Insights gained from 16S rDNA sequencing further illuminated that the integration of probiotics and dark tea was capable of reshaping the intestinal microbiota landscape, notably augmenting the Firmicutes/Bacteroidetes ratio within the gut of constipated mice.This shift was particularly evident in the proliferation of Lactobacillus, underscoring the potential of this combination in fostering a favorable gut microbial ecosystem.In conclusion, the synergize of probiotics with dark tea emerged as an effective strategy for mitigating loperamide hydrochloride-induced constipation in mice.This combined approach not only repaired intestinal barrier impairments but also exerted a modulatory influence on the composition and architecture of the intestinal microbiota, thereby contributing to a healthier gut environment.This study offers a novel research perspective and serves as a pivotal reference, not only broadening the horizons of probiotics research but also pioneering the development of innovative functional foods aimed at intervening in and mitigating constipation, thereby contributing significantly to the advancement of this field.
[1] HAYAT U, DUGUM M, GARG S.Chronic constipation:Update on management[J].Cleveland Clinic Journal of Medicine, 2017, 84(5):397-408.
[2] 刘宝华. 《便秘外科诊治指南》(2017年版)解读[J].中华胃肠外科杂志, 2017(12):1331-1333.
LIU B H.Interpretation of Chinese guidelines for the diagnosis and treatment of constipation surgery (2017)[J].Chinese Journal of Gastrointestinal Surgery, 2017(12):1331-1333.
[3] 李俊洁, 陈庆森.双歧杆菌调理和改善肠道相关疾病作用的研究进展[J].食品科学, 2011, 32(23):326-332.
LI J J, CHEN Q S.Recent advances in research on the role of bifidobacteria in regulating and improving gut-associated diseases[J].Food Science, 2011, 32(23):326-332.
[4] BOKIC T, STORR M, SCHICHO R.Potential causes and present pharmacotherapy of irritable bowel syndrome:An overview[J].Pharmacology, 2015, 96(1-2):76-85.
[5] RAO T P, QUARTARONE G.Role of guar fiber in improving digestive health and function[J].Nutrition, 2019, 59:158-169.
[6] YOON J Y, CHA J M, OH J K, et al.Probiotics ameliorate stool consistency in patients with chronic constipation:A randomized, double-blind, placebo-controlled study[J].Digestive Diseases and Sciences, 2018, 63(10):2754-2764.
[7] YANG W Q, REN D Y, ZHAO Y, et al.Fuzhuan brick tea polysaccharide improved ulcerative colitis in association with gut microbiota-derived tryptophan metabolism[J].Journal of Agricultural and Food Chemistry, 2021, 69(30):8448-8459.
[8] LUO J Y, YAN D, SONG J Y, et al.A strategy for trade monitoring and substitution of the organs of threatened animals[J].Scientific Reports, 2013, 3:3108.
[9] CHEN J Q, LI D W, CHEN Y Y, et al.Elucidating dosage-effect relationship of different efficacy of rhubarb in constipation model rats by factor analysis[J].Journal of Ethnopharmacology, 2019, 238:111868.
[10] QIAN Y, SONG J L, YI R K, et al.Preventive effects of Lactobacillus plantarum YS4 on constipation induced by activated carbon in mice[J].Applied Sciences, 2018, 8(3):363.
[11] 彭昊, 龙博文, 肖晨, 等.重症监护室患儿血清胃泌素和胃动素指标的变化及意义研究[J].中国全科医学, 2021, 24(32):4094-4098;4109.
PENG H, LONG B W, XIAO C, et al.Changes and significance of serum gastrin and motilin in the blood of children in pediatric intensive care unit[J].Chinese General Practice, 2021, 24(32):4094-4098;4109.
[12] FUJIMURA K E, SLUSHER N A, CABANA M D, et al.Role of the gut microbiota in defining human health[J].Expert Review of Anti-Infective Therapy, 2010, 8(4):435-454.
[13] 孙祥珍, 马臻棋, 马雪芹.初探VIP、P物质与便秘的关系[J].世界最新医学信息文摘,2019,19(26):113-114.
SUN X Z, MA Z Q, MA X Q.Preliminary study on the relationship between VIP,substance P and constipation[J].World Latest Medicine Information,2019,19(26):113-114.
[14] 李冬华, 朱飞鹏, 李珊珊, 等.疏肝健脾法调控肠易激综合征动物模型胃肠激素水平的研究[J].中国实验方剂学杂志, 2008, 14(7):20-23.
LI D H, ZHU F P, LI S S, et al.Study on the method of soothing the liver and strengthening the spleen on regulating gastrointestinal hormones in animals model of irritable bowel syndrome[J].Chinese Journal of Experimental Traditional Medical Formulae, 2008, 14(7):20-23.
[15] ZOU J F, SHEN Y M, CHEN M J, et al.Lizhong decoction ameliorates ulcerative colitis in mice via modulating gut microbiota and its metabolites[J].Applied Microbiology and Biotechnology, 2020, 104(13):5999-6012.
[16] PLAZA-DÍAZ J, RUIZ-OJEDA F J, VILCHEZ-PADIAL L M, et al.Evidence of the anti-inflammatory effects of probiotics and synbiotics in intestinal chronic diseases[J].Nutrients, 2017, 9(6):555.
[17] DIBAISE J K, FRANK D N, MATHUR R.Impact of the gut microbiota on the development of obesity:Current concepts[J].The American Journal of Gastroenterology Supplements, 2012, 1(1):22-27.
[18] KHALIF I L, QUIGLEY E M M, KONOVITCH E A, et al.Alterations in the colonic flora and intestinal permeability and evidence of immune activation in chronic constipation[J].Digestive and Liver Disease, 2005, 37(11):838-849.
[19] 罗真东, 梁鉴坤, 王哲, 等.小麦纤维素联合乳果糖治疗儿童功能性便秘的临床观察[J].中国现代医学杂志, 2018, 28(36):96-99.
LUO Z D, LIANG J K, WANG Z, et al.Application of Fiberform and Lactulose in treatment of functional constipation in children[J].China Journal of Modern Medicine, 2018, 28(36):96-99.
[20] MARIAT D, FIRMESSE O, LEVENEZ F, et al.The Firmicutes/Bacteroidetes ratio of the human microbiota changes with age[J].BMC Microbiology, 2009, 9:123.
[21] SHIDA K, MAKINO K, MORISHITA A, et al.Lactobacillus casei inhibits antigen-induced IgE secretion through regulation of cytokine production in murine splenocyte cultures[J].International Archives of Allergy and Immunology, 1998, 115(4):278-287.
[22] 彭芝榕, 刘晓梅, 倪学勤, 等.植物乳杆菌F1208对便秘大鼠胃肠道功能的影响[J].食品科学, 2013, 34(13):243-246.
PENG Z R, LIU X M, NI X Q, et al.Effect of Lactobacillus plantarum F1208 on gastrointestinal functions in constipated rats[J].Food Science, 2013, 34(13):243-246.
[23] 刘晓梅, 彭芝榕, 倪学勤, 等.低聚果糖、乳酸杆菌对便秘模型大鼠的通便功能影响[J].食品科学, 2013, 34(11):296-299.
LIU X M, PENG Z R, NI X Q, et al.Aperient effect of fructooligosaccharides and Lactobacillus on constipation model of rats[J].Food Science, 2013, 34(11):296-299.
[24] 詹宏杰, 梁寒.肠道菌群与结直肠癌关系研究进展[J].中国肿瘤临床, 2015, 42(13):675-679.
ZHAN H J, LIANG H.Research progress on the relationship between intestinal microflora and colorectal cancer[J].Chinese Journal of Clinical Oncology, 2015, 42(13):675-679.
