研究报告

植物乳植杆菌CN2018对L-精氨酸诱导的大鼠胃动力障碍的影响

  • 孙新凯 ,
  • 毛丙永 ,
  • 唐鑫 ,
  • 张秋香 ,
  • 崔树茂 ,
  • 赵建新
展开
  • (江南大学 食品学院,江苏 无锡,214122)
第一作者: 博士研究生(赵建新教授为通信作者,E-mail:zhaojianxin@jiangnan.edu.cn)

收稿日期: 2024-07-22

  修回日期: 2024-08-29

  网络出版日期: 2025-08-04

基金资助

国家自然科学基金面上项目(32372300)

Effects of Lactiplantibacillus plantarum CN2018 on rats with L-arginine induced gastric motility disorders

  • SUN Xinkai ,
  • MAO Bingyong ,
  • TANG Xin ,
  • ZHANG Qiuxiang ,
  • CUI Shumao ,
  • ZHAO Jianxin
Expand
  • (School of Food Science and Technology, Jiangnan University, Wuxi 214122, China)

Received date: 2024-07-22

  Revised date: 2024-08-29

  Online published: 2025-08-04

摘要

植物乳植杆菌CN2018是一株具有抗幽门螺杆菌感染功能的专利菌,其对胃动力的影响尚不清楚。该研究采用L-精氨酸诱导SD大鼠胃动力障碍模型探究了植物乳植杆菌CN2018对胃动力的改善效果。结果发现,与模型组相比,植物乳植杆菌CN2018可显著增加胃动力障碍大鼠的胃排空率,并具有增加胃液量、胃酸排出速率和胃蛋白酶活性、及降低胃酸分泌的趋势;同时,植物乳植杆菌CN2018显著增加胃动力障碍大鼠饥饿素和胃泌素的分泌,显著提高了大鼠胃窦组织酪氨基酶受体基因(c-Kit)和干细胞因子的转录水平。综上,植物乳植杆菌CN2018对改善胃动力障碍具有良好效果。

本文引用格式

孙新凯 , 毛丙永 , 唐鑫 , 张秋香 , 崔树茂 , 赵建新 . 植物乳植杆菌CN2018对L-精氨酸诱导的大鼠胃动力障碍的影响[J]. 食品与发酵工业, 2025 , 51(13) : 69 -74 . DOI: 10.13995/j.cnki.11-1802/ts.040535

Abstract

Lactiplantibacillus plantarum CN2018 is a patented strain capable of resisting infection of Helicobacter pylori, but its effect on gastric motility is still unknown.In this study, we investigated the effect of L.plantarum CN2018 on L-arginine-induced gastric motility disorder in SD rats.The results showed that compared with the model group, L.plantarum CN2018 significantly increased the gastric emptying rate of rats with gastric motility disorders, and showed the trend to increase the volume of gastric juice, the rate of gastric acid excretion and the activity of pepsin, as well as reducing the secretion of gastric acid.Meanwhile, L.plantarum CN2018 significantly increased the transcription levels of tyrosinase receptor gene (c-Kit) and stem cell factor (SCF) in rat gastric antrum tissue.Overall, L.plantarum CN2018 has a good effect in improving gastric motility disorders.

参考文献

[1] WANG X, ZHANG C, ZHENG M Y, et al.Metabolomics analysis of L-arginine induced gastrointestinal motility disorder in rats using UPLC-MS after magnolol treatment[J].Frontiers in Pharmacology, 2019, 10:183.
[2] CAMILLERI M, ATIEH J.New developments in prokinetic therapy for gastric motility disorders[J].Frontiers in Pharmacology, 2021, 12:711500.
[3] COATE K C, KLIEWER S A, MANGELSDORF D J.SnapShot:Hormones of the gastrointestinal tract[J].Cell, 2014, 159(6):1478.
[4] SPENCER N J, HU H Z.Enteric nervous system:Sensory transduction, neural circuits and gastrointestinal motility[J].Nature Reviews.Gastroenterology & Hepatology, 2020, 17(6):338-351.
[5] GIUDICESSI J R, ACKERMAN M J, CAMILLERI M.Cardiovascular safety of prokinetic agents:A focus on drug-induced arrhythmias[J].Neurogastroenterology & Motility, 2018, 30(6):e13302.
[6] MARGOLIS K G, CRYAN J F, MAYER E A.The microbiota-gut-brain axis:From motility to mood[J].Gastroenterology, 2021, 160(5):1486-1501.
[7] WACLAWIKOVÁ B, CODUTTI A, ALIM K, et al.Gut microbiota-motility interregulation:Insights from in vivo, ex vivo and in silico studies[J].Gut Microbes, 2022, 14(1):1997296.
[8] ZHENG Z P, TANG J Y, HU Y N, et al.Role of gut microbiota-derived signals in the regulation of gastrointestinal motility[J].Frontiers in Medicine, 2022, 9:961703.
[9] SUI S J, TIAN Z B, WANG Q C, et al.Clostridium butyricum promotes intestinal motility by regulation of TLR2 in interstitial cells of Cajal[J].European Review for Medical and Pharmacological Sciences, 2018, 22(14):4730-4738.
[10] CHANDRASEKHARAN B, SAEEDI B J, ALAM A, et al.Interactions between commensal bacteria and enteric neurons, via FPR1 induction of ROS, increase gastrointestinal motility in mice[J].Gastroenterology, 2019, 157(1):179-192.
[11] ZHANG L M, ZENG J, MA Y Y, et al.Effects of Synbiotic2000TM forte on the intestinal motility and interstitial cells of Cajal in TBI mouse model[J].Probiotics and Antimicrobial Proteins, 2017, 9(2):172-181.
[12] INDRIO F, RIEZZO G, RAIMONDI F, et al.Lactobacillus reuteri accelerates gastric emptying and improves regurgitation in infants[J].European Journal of Clinical Investigation, 2011, 41(4):417-422.
[13] INDRIO F, RIEZZO G, GIORDANO P, et al.Effect of a partially hydrolysed whey infant formula supplemented with starch and Lactobacillus reuteri DSM 17938 on regurgitation and gastric motility[J].Nutrients, 2017, 9(11):1181.
[14] 陈卫, 田丰伟, 陈晓华, 等.一种抗幽门螺杆菌感染的植物乳杆菌及其用途:中国, ZL 201110059827.6[P].2011-09-07.
CHEN W, TIAN F W, CHEN X H, et al.A strain of Lactobacillus plantarum capable of resisting infection of Helicobacter pylori and its applications:China, ZL 201110059827.6[P].2011-09-07.
[15] DENG Y Z, ZHOU X Y, XIANG X C, et al.Effect of miRNA-19a on gastrointestinal motility in rats with functional dyspepsia[J].Experimental and Therapeutic Medicine, 2018, 15(6):4875-4879.
[16] 林飞, 梁怿, 吕廉, 等.富硒大蒜对实验动物上消化道的刺激作用及对胃液成分的影响[J].癌变·畸变·突变, 2008, 20(4):275-278.
LIN F, LIANG Y, LU L, et al.Selenium-enriched garlic on the gastric juice components and upper gastrointestinal tract stimulation in experimental animal[J].Carcinogenesis, Teratogenesis & Mutagenesis, 2008, 20(4):275-278.
[17] XIAO Z L, XU J, TAN J, et al.Zhizhu Kuanzhong, a traditional Chinese medicine, alleviates gastric hypersensitivity and motor dysfunction on a rat model of functional dyspepsia[J].Frontiers in Pharmacology, 2022, 13:1026660.
[18] 杨伟金, 杨明华, 陈婉姬.小儿健脾膏对胃动力障碍大鼠胃肠组织中胃动素m RNA和蛋白表达的影响[J].中国现代应用药学, 2021, 38(21):2654-2658.
YANG W J, YANG M H, CHEN W J.Effect of Xiao’er Jianpi plaster on motilin mRNA and protein expression in stomach and intestine of rats with gastric motility disorder[J].Chinese Journal of Modern Applied Pharmacy, 2021, 38(21):2654-2658.
[19] SCHUBERT M L.Physiologic, pathophysiologic, and pharmacologic regulation of gastric acid secretion[J].Current Opinion in Gastroenterology, 2017, 33(6):430-438.
[20] 邓葵, 刘纯伦.胃酸与功能性消化不良[J].胃肠病学和肝病学杂志, 2016, 25(9):1058-1061.
DENG K, LIU C L.Gastric acid and functional dyspepsia[J].Chinese Journal of Gastroenterology and Hepatology, 2016, 25(9):1058-1061.
[21] DEL RIO A R, KEPPLER J K, BOOM R M, et al.Protein acidification and hydrolysis by pepsin ensure efficient trypsin-catalyzed hydrolysis[J].Food & Function, 2021, 12(10):4570-4581.
[22] ARIN R M, GOROSTIDI A, NAVARRO-IMAZ H, et al.Adenosine:Direct and indirect actions on gastric acid secretion[J].Frontiers in Physiology, 2017, 8:737.
[23] RICH A, MILLER S M, GIBBONS S J, et al.Local presentation of Steel factor increases expression of c-kit immunoreactive interstitial cells of Cajal in culture[J].American Journal of Physiology.Gastrointestinal and Liver Physiology, 2003, 284(2):G313-G320.
[24] ZHENG X W, ZHANG Y X, TAN Y F, et al.Alpinia officinarum Hance extract ameliorates diabetic gastroparesis by regulating SCF/c-kit signaling pathway and rebalancing gut microbiota[J].Fitoterapia, 2024, 172:105730.
文章导航

/