该研究旨在评估植物乳植杆菌CCFM1351对侧柏叶防脱护发功能的影响。细胞实验发现,植物乳植杆菌CCFM1351发酵侧柏叶代谢产物(5 mg/mL)显著提高了人毛囊真皮乳头细胞的活力,上调了胰岛素生长因子-1(insulin-like growth factor 1,IGF-1)和Cyclin D的mRNA表达。在脱发小鼠模型中,CCFM1351发酵侧柏叶组小鼠背部毛发的再生显著增加,毛发覆盖率在第21天达到最高值。进一步研究发现,CCFM1351活菌组与合生制剂组(CCFM1351活菌+侧柏叶提取物)上调了Wnt10b的mRNA表达,合生制剂组上调了小鼠皮肤中Wnt/β-catenin信号通路的关键蛋白β-连环蛋白(β-catenin)的表达,显著提高了血管内皮生长因子(vascular endothelial growth factor,VEGF)和IGF-1的含量,并下调了转化生长因子β2(transforming growth factor,TGF-β2)和BAX的表达。以上结果表明,植物乳植杆菌CCFM1351能够协同侧柏叶,通过Wnt/β-catenin信号通路,促进毛发生长并抑制毛发凋亡,对侧柏叶防脱护发有协同促进作用,在脱发治疗中具有应用潜力。
This study aimed to evaluate the effects of Lactiplantibacillus plantarum CCFM1351 on the anti-hair loss and hair care functions of Cacumen platycladi extract.In vitro cell experiments revealed that fermented C. platycladi extract by L.plantarum CCFM1351 (5 mg/mL) significantly increased the viability of human hair follicle dermal papilla cells (HDPCs) and upregulated the mRNA expression of Insulin-like growth factor 1 (IGF-1) and Cyclin D.In the hair loss mouse model, mice treated with CCFM1351 fermented C. platycladi extract exhibited significant hair regrowth on their backs, with the highest hair coverage observed on the 21st day.Further study revealed that live CCFM1351 group and the synbiotic group (live CCFM1351+C. platycladi extract) upregulated Wnt10b mRNA expression.The synbiotic group also significantly enhanced the expression of β-catenin, a key protein in the Wnt/β-catenin signaling pathway, and increased the levels of Vascular endothelial growth factor (VEGF) and IGF-1, while reducing the expression of Transforming growth factor (TGF-β2) and BAX in the skin.The results indicate that L.plantarum CCFM1351 can synergistically promote hair growth and inhibit hair apoptosis in C. platycladi extract through the Wnt/β-catenin signaling pathway.It has a synergistic effect on preventing hair loss and hair care in C. platycladi extract, and has potential application in hair loss treatment.
[1] 刘起彬, 高建, 谢林海.雄激素性脱发的治疗现状[J].中国美容整形外科杂志, 2023, 34(9):576-579.
LIU Q B, GAO J, XIE L H.Current status of treatment of androgenetic alopecia [J].Chinese Journal of Aesthetic and Plastic Surgery, 2023, 34(9):576-579.
[2] CHOI Y S, ZHANG Y H, XU M G, et al.Distinct functions for Wnt/β-catenin in hair follicle stem cell proliferation and survival and interfollicular epidermal homeostasis[J].Cell Stem Cell, 2013, 13(6):720-733.
[3] CHOI B Y.Targeting Wnt/β-catenin pathway for developing therapies for hair loss[J].International Journal of Molecular Sciences, 2020, 21(14):4915.
[4] 刘青, 黄芳.侧柏、姜、红车轴草、艾复合提取物的防脱发功效[J].广东化工, 2022, 49(22):76-78.
LIU Q, HUANG F.Anti-hair loss effect of a mixture comprising extracts of arborvitae, ginger, clover and mugwort[J].Guangdong Chemical Industry, 2022, 49(22):76-78.
[5] 马世宏, 单承莺, 聂韡, 等.侧柏叶、何首乌复合提取物防脱发功效研究[J].中国野生植物资源, 2021, 40(5):43-47.
MA S H, SHAN C Y, NIE W, et al.Study on the anti-hair loss effects of the compound extract of Platycladus orientalis and Polygonum multiflorum[J].Chinese Wild Plant Resources, 2021, 40(5):43-47.
[6] YIN C S, MINH NGUYEN T T, YI E J, et al.Efficacy of probiotics in hair growth and dandruff control:A systematic review and meta-analysis[J].Heliyon, 2024, 10(9):e29539.
[7] LAM S, ZHANG J W, YANG K L, et al.Modulation of gut microbiota impacts diet-induced and drug-induced alopecia in mice[J].Gut, 2022, 71(11):2366-2369.
[8] TSAI W H, FANG Y T, HUANG T Y, et al.Heat-killed Lacticaseibacillus paracasei GMNL-653 ameliorates human scalp health by regulating scalp microbiome[J].BMC Microbiology, 2023, 23(1):121.
[9] CHAE M, KIM B J, NA J, et al.Antimicrobial activity of Lactiplantibacillus plantarum APsulloc 331261 and APsulloc 331266 against pathogenic skin microbiota[J].Frontiers in Bioscience, 2021, 13(2):237-248.
[10] LEE Y I, HAM S, LEE S G, et al.An exploratory in vivo study on the effect of annurca apple extract on hair growth in mice[J].Current Issues in Molecular Biology, 2022, 44(12):6280-6289.
[11] 苏胤杰, 杨劲, 张艺, 等.毛乳头细胞的研究现状[J].医学综述, 2014, 20(1):12-14.
SU Y J, YANG J, ZHANG Y, et al.The cell of dermal papilla at a glance[J].Medical Recapitulate, 2014, 20(1):12-14.
[12] 沈胡驰, 陈殿松, 杨井国, 等.茶麸黄酮促进毛乳头细胞增殖和VEGF分泌的研究[J].日用化学工业, 2022, 52(1):44-49.
SHEN H C, CHEN D S, YANG J G, et al.Study on the promotion of proliferation of dermal papilla cells and VEGF secretion by flavonoids from camellia seed cake[J].China Surfactant Detergent & Cosmetics, 2022, 52(1):44-49.
[13] WEGER N, SCHLAKE T.IGF-I signalling controls the hair growth cycle and the differentiation of hair shafts[J].Journal of Investigative Dermatology, 2005, 125(5):873-882.
[14] TRÜEB R M.Further clinical evidence for the effect of IGF-1 on hair growth and alopecia[J].Skin Appendage Disorders, 2018, 4(2):90-95.
[15] KIM J, SHIN J Y, CHOI Y H, et al.Hair growth promoting effect of hottuynia cordata extract in cultured human hair follicle dermal papilla cells[J].Biological & Pharmaceutical Bulletin, 2019, 42(10):1665-1673.
[16] DANILENKO D M, RING B D, PIERCE G F.Growth factors and cytokines in hair follicle development and cycling:Recent insights from animal models and the potentials for clinical therapy[J].Molecular Medicine Today, 1996, 2(11):460-467.
[17] INUI S, ITAMI S.Molecular basis of androgenetic alopecia:From androgen to paracrine mediators through dermal papilla[J].Journal of Dermatological Science, 2011, 61(1):1-6.
[18] MACDONALD B T, TAMAI K, HE X.Wnt/β-catenin signaling:Components, mechanisms, and diseases[J].Developmental Cell, 2009, 17(1):9-26.
[19] SOMA T, FUJIWARA S, SHIRAKATA Y, et al.Hair-inducing ability of human dermal papilla cells cultured under Wnt/β-catenin signalling activation[J].Experimental Dermatology, 2012, 21(4):307-309.
[20] PERKINS R S, SINGH R, ABELL A N, et al.The role of WNT10B in physiology and disease:A 10-year update[J].Frontiers in Cell and Developmental Biology, 2023, 11:1120365.
[21] VOGLER M, DINSDALE D, DYER M S, et al.Bcl-2 inhibitors:Small molecules with a big impact on cancer therapy[J].Cell Death and Differentiation, 2009, 16(3):360-367.
[22] WEI L Y, LI Y M, HAO Z N, et al.Fermentation improves antioxidant capacity and γ-aminobutyric acid content of Ganmai Dazao Decoction by lactic acid bacteria[J].Frontiers in Microbiology, 2023, 14:1274353.
