研究报告

改性方法对黑小麦麸皮戊聚糖理化性质和功能特性的影响

  • 朱慧慧 ,
  • 郭佳怡 ,
  • 刘瑞 ,
  • 李楠 ,
  • 孙元琳
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  • 1(山西农业大学 食品科学与工程学院,山西 太谷,030801)
    2(运城学院 生命科学系,山西 运城,044000)
    3(山西省特优农产品梯次高值利用技术创新中心,山西 运城,044000)
第一作者:硕士研究生(孙元琳教授为通信作者,E-mail:sylwts@aliyun.com)

收稿日期: 2024-07-28

  修回日期: 2024-12-20

  网络出版日期: 2025-08-29

基金资助

国家自然科学基金项目(32072222);山西省技术创新中心基地项目(202404010920021)

Effects of modification methods on physicochemical properties and functional properties of pentosan from black wheat bran

  • ZHU Huihui ,
  • GUO Jiayi ,
  • LIU Rui ,
  • LI Nan ,
  • SUN Yuanlin
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  • 1(College of Food Science and Engineering, Shanxi Agricultural University, Taigu 030801, China)
    2(Life Science Department, Yuncheng University, Yuncheng 044000, China)
    3(Shanxi Technology Innovation Center of High Value-Added Echelon Utilization of Premium Agro-Products, Yuncheng 044000, China)

Received date: 2024-07-28

  Revised date: 2024-12-20

  Online published: 2025-08-29

摘要

以黑小麦麸皮为原料,对其进行微波-挤压和微波-挤压-酶解处理,采用水法和碱法提取戊聚糖,探究改性方法对戊聚糖理化性质和功能活性的影响。结果显示,各戊聚糖组分均主要由阿拉伯糖(arabinose,Ara)、木糖(xylose,Xyl)、葡萄糖(glucose,Glc)以及微量半乳糖(galactose,Gal)组成,改性后戊聚糖组分中Ara和Xyl含量降低,Glc含量增加。戊聚糖组分的相对分子质量随着改性处理有所下降,其微观结构变得疏松多孔,表面粗糙,颗粒化或碎片化明显。改性后戊聚糖的理化性质得到改善,体外抗氧化、降糖降脂活性显著增强。微波-挤压-酶解碱提组分的亚硝酸根离子吸附能力、自由基清除能力和总还原能力最强。体外降血糖结果显示,微波-挤压-酶解碱提组分的α-葡萄糖苷酶抑制率最高,较未处理组分提高了69%;微波-挤压-酶解水提组分的α-淀粉酶率最高为49.77%。此外,微波-挤压-酶解水提组分对牛磺胆酸钠的吸附率最高为65.62%,是未处理组分的2.08倍。综上,微波-挤压-酶解处理能有效改善戊聚糖的理化性质和功能活性,为黑小麦麸皮资源的利用及其功能食品研发提供理论依据。

本文引用格式

朱慧慧 , 郭佳怡 , 刘瑞 , 李楠 , 孙元琳 . 改性方法对黑小麦麸皮戊聚糖理化性质和功能特性的影响[J]. 食品与发酵工业, 2025 , 51(16) : 81 -89 . DOI: 10.13995/j.cnki.11-1802/ts.040593

Abstract

Using black wheat bran as raw material, treated it by microwave-extrusion and microwave-extrusion-enzymatic hydrolysis,The pentosan of black wheat bran was extracted by water method and alkali method, and the effects of modification methods on the physicochemical properties and functional activity of pentosan were investigated.Results showed that each pentosan component was mainly composed of Ara, Xyl, Glc, and trace Gal.After modification, the content of Ara and Xyl in the pentosan fraction decreased, and the content of Glc increased.The relative molecular mass of pentosan components decreased with the modification treatment, and its microstructure became loose and porous, the surface was rough, and the granulation or fragmentation was obvious.The physical and chemical properties of the modified pentosan were improved, and the antioxidant, hypoglycemic, and lipid-lowering activities in vitro were significantly enhanced.The microwave-extrusion-enzymatic alkali extraction components had the strongest nitrite ion adsorption capacity, free radical scavenging ability, and total reduction ability.The results of hypoglycemic in vitro showed that the α-glucosidase inhibition rate of the microwave-extrusion-enzymatic alkali extraction component was the highest, which was 69% higher than that of the untreated component.The highest α-amylase rate of microwave-extrusion-enzymatic water extraction components was 49.77%.In addition, the highest adsorption rate of sodium taurocholate by microwave-extrusion-enzymatic hydrolysis was 65.62%, which was 2.08 times that of untreated components.In summary, microwave-extrusion-enzymatic treatment can effectively improve the physicochemical properties and functional activity of pentosan, and provide a theoretical basis for the utilization of black wheat bran resources and the development of functional foods.

参考文献

[1] 裴自友, 孙玉, 孙善澄, 等.中国黑小麦研究利用现状[J].种子, 2002, 21(4):42-44.
PEI Z Y, SUN Y, SUN S C, et al.Besearch progress of Chinese black wheat[J].Seed, 2002, 21(4):42-44.
[2] 李帮秀, 宗学凤, 张建奎.黑粒小麦的营养特性研究[J].西南农业大学学报(自然科学版), 2004, 26(6):753-755.
LI B X, ZONG X F, ZHANG J K, et al.Study on nutritional characters of whent genotypes with different grain color[J].Journal of Southwest Agricultural University (Natural Science Edition), 2004, 26(6):753-755.
[3] 张慧娟, 靳程茗, 吕世豪, 等.黑麦麸膳食纤维的结构和功能特性研究[J].中国食品学报, 2023, 23(8):84-93.
ZHANG H J, JIN C M, LYU S H, et al.Studies on structure and functional characteristics of dietary fiber from rye bran[J].Journal of Chinese Institute of Food Science and Technology, 2023, 23(8):84-93.
[4] 张茜, 赵鑫, 成锦华, 等.基于核磁共振氢谱代谢组学研究阿拉伯木聚糖及其复合多糖对高脂饮食诱导肥胖小鼠粪便代谢物的影响[J].食品科学, 2023, 44(19):140-147.
ZHANG Q, ZHAO X, CHENG J H, et al.Proton nuclear magnetic resonance spectroscopy (1H-NMR)-based metabolomic analysis effects of arabinoxylan and its mixture with β-glucan on fecal metabolites in high-fat diet-induced obese mice[J].Food Science, 2023, 44(19):140-147.
[5] NIE Q X, HU J L, CHEN H H,et al.Arabinoxylan ameliorates type 2 diabetes by regulating the gut microbiota and metabolites[J].Food Chemistry, 2022, 371:131106.
[6] CAO L, LIU X Z, QIAN T X, et al.Antitumor and immunomodulatory activity of arabinoxylans:A major constituent of wheat bran[J].International Journal of Biological Macromolecules, 2011, 48(1):160-164.
[7] 李凡, 李言, 钱海峰, 等.阿拉伯木聚糖提取及其对面团性质影响研究进展[J].中国粮油学报, 2022, 37(6):194-202.
LI F, LI Y, QIAN H F, et al.Research progress on extraction of arabinoxylan and its effects on dough[J].Journal of the Chinese Cereals and Oils Association, 2022, 37(6):194-202.
[8] ELLEUCH M, BEDIGIAN D, ROISEUX O, et al.Dietary fibre and fibre-rich by-products of food processing:Characterisation, technological functionality and commercial applications:A review[J].Food Chemistry, 2011, 124(2):411-421.
[9] DAMEN B, VERSPREET J, POLLET A, et al.Prebiotic effects and intestinal fermentation of cereal arabinoxylans and arabinoxylan oligosaccharides in rats depend strongly on their structural properties and joint presence[J].Molecular Nutrition & Food Research, 2011, 55(12):1862-1874.
[10] 田玉荣. 不同加工工艺条件下黑小麦麸皮戊聚糖的理化性质及益生活性研究[D].太原:山西大学, 2021.
TIAN Y R.Study on the physicochemical properties and prebiotic activity of black wheat bran pentosan under different processing conditions[D].Taiyuan:Shanxi University, 2021.
[11] 吴丽萍, 董康珍, 楚文靖, 等.微波改性对燕麦麸膳食纤维结构及功能性质的影响[J].中国食品学报, 2021, 21(9):30-37.
WU L P, DONG K Z, CHU W J, et al.Effects of microwave modification on the structure and functional properties of oat bran dietary fiber[J].Journal of Chinese Institute of Food Science and Technology, 2021, 21(9):30-37.
[12] 袁志宁, 霍栓, 严嘉恬, 等.挤压对柚皮可溶性膳食纤维理化、功能及结构特性的影响[J].天津科技大学学报, 2024, 39(3):21-27;71.
YUAN Z N, HUO S, YAN J T, et al.Effect of extrusion on physicochemical, functional and structural properties of dietary fiber from pomelo peel[J].Journal of Tianjin University of Science & Technology, 2024, 39(3):21-27;71.
[13] 魏旭瑶, 姜彩霞, 曾祥瑞, 等.复合酶改性玉米皮可溶性膳食纤维的工艺优化及理化特性分析[J].食品工业科技, 2024, 45(6):202-209.
WEI X Y, JIANG C X, ZENG X R, et al.Process optimization and physicochemical characteristics analysis of soluble dietary fiber from corn bran modified by composite enzymes[J].Science and Technology of Food Industry, 2024, 45(6):202-209.
[14] 温启华, 陆逸昊, 杨露芳, 等.硒化天麻多糖的制备、结构表征及其抗氧化活性评价[J].食品工业科技, 2024, 45(3):18-30.
WEN Q H, LU Y H, YANG L F, et al.Preparation, structure characterization and antioxidant activity evaluation of selenized Gastrodia elata polysaccharides[J].Science and Technology of Food Industry, 2024, 45(3):18-30.
[15] 谢佳函, 刘回民, 刘美宏, 等.红豆皮多酚提取工艺优化及抗氧化活性分析[J].中国食品学报, 2020, 20(1):147-157.
XIE J H, LIU H M, LIU M H, et al.Extraction process optimization and antioxidant activity analysis of polyphenols from azuki bean coats (Vigna angularis)[J].Journal of Chinese Institute of Food Science and Technology, 2020, 20(1):147-157.
[16] LEE J H, HWANG C E, SON K S, et al.Comparisons of nutritional constituents in soybeans during solid state fermentation times and screening for their glucosidase enzymes and antioxidant properties[J].Food Chemistry, 2019,272:362-371.
[17] 陈薛. 不同茶树品种鲜叶多糖的理化性质和抗氧化活性比较研究[D].杭州:浙江大学, 2022.
CHEN X. Comparative study on physicochemical properties and antioxidant activity of polysaccharides from fresh leaves of different tea varieties[D]. Hangzhou: Zhejiang University, 2022.
[18] ZHENG Q W, JIA R B, LUO D H, et al.The effect of extraction solution pH level on the physicochemical properties and α-glucosidase inhibitory potential of Fucus vesiculosus polysaccharide[J].LWT, 2022, 169:114028.
[19] SUN L J, CHEN W Q, MENG Y H, et al.Interactions between polyphenols in thinned young apples and porcine pancreatic α-amylase:Inhibition, detailed kinetics and fluorescence quenching[J].Food Chemistry, 2016, 208:51-60.
[20] WANG C H, MA Y L, ZHU D Y, et al.Physicochemical and functional properties of dietary fiber from bamboo shoots (Phyllostachys praecox)[J].Emirates Journal of Food and Agriculture, 2017, 29(7):509-517.
[21] 于美汇, 赵鑫, 尹红力, 等.碱提醇沉黑木耳多糖体外和体内降血脂功能[J].食品科学, 2017, 38(1):232-237.
YU M H, ZHAO X, YIN H L, et al.In vitro and in vivo hypolipidemic effect of Auricularia auricular polysaccharides[J].Food Science, 2017, 38(1):232-237.
[22] 段振. 石榴皮不溶性膳食纤维的提取、体外降血脂活性研究及咀嚼片制备[D].西安:陕西师范大学, 2018.
DUAN Z.Extraction of insoluble dietary fiber from pomegranate peel, study on its hypolipidemic activity in vitro and preparation of chewable tablets[D].Xi’an:Shaanxi Normal University, 2018.
[23] 王大为, 宋云禹, 刘阳, 等.毛葱膳食纤维性质及结构分析[J].食品科学, 2018, 39(2):53-57.
WANG D W, SONG Y Y, LIU Y, et al.Properties and structure of dietary fiber from tillering onion[J].Food Science, 2018, 39(2):53-57.
[24] 张帅, 郭晓雪, 任丽琨, 等.酶法改性影响膳食纤维的构成及生物作用效果的研究进展[J].食品安全质量检测学报, 2022, 13(4):1089-1098.
ZHANG S, GUO X X, REN L K, et al.Research progress of enzymatic modification on the composition and biological effects of dietary fiber[J].Journal of Food Safety & Quality, 2022, 13(4):1089-1098.
[25] 郑佳欣. 刺梨渣膳食纤维蒸汽爆破改性及结构、功能性质研究[D].北京:北京林业大学, 2020.
ZHENG J X.Study on steam explosion modification, structure and functional properties of dietary fiber from Rosa roxburghii Tratt residue[D].Beijing:Beijing Forestry University, 2020.
[26] 杨开, 杨振寰, 吴伟杰, 等.雷笋膳食纤维酶法改性及其理化性能和结构变化[J].食品与发酵工业, 2019, 45(4):36-41.
YANG K, YANG Z H, WU W J, et al.Physicochemical properties and structural changes of bamboo shoots dietary fiber with enzymatic modification[J].Food and Fermentation Industries, 2019, 45(4):36-41.
[27] WU D T, HE Y, FU M X, et al.Structural characteristics and biological activities of a pectic-polysaccharide from okra affected by ultrasound assisted metal-free Fenton reaction[J].Food Hydrocolloids, 2022, 122:107085.
[28] 王俊杰, 罗钰湲, 黄越, 等.球磨-酶法联合工艺制备菠萝皮渣不溶性膳食纤维:结构与理化特性的研究[J].食品与发酵工业, 2025,51(4):162-169.
WANG J J, LUO Y Y, HUANG Y, et al.Preparation of insoluble dietary fiber from pineapple peel by ball milling enzymatic combined method:Study on structure and physicochemical properties[J].Food and Fermentation Industries, 2025,51(4):162-169.
[29] 吴鸿亿, 黄玲冬, 刘奇, 等.制备方法对高节竹笋头可溶性膳食纤维的理化与功能特性影响[J].食品与发酵工业, 2025,51(2):258-266.
WU H Y, HUANG L D, LIU Q, et al.Effects of preparation methods on physicochemical and functional properties of soluble dietary fiber from Phyllostachys prominens bamboo shoot head[J].Food and Fermentation Industries, 2025,51(2):258-266.
[30] 任雨离, 刘玉凌, 何翠, 等.微波和微粉碎改性对方竹笋膳食纤维性能和结构的影响[J].食品与发酵工业, 2017, 43(8):145-150.
REN Y L, LIU Y L, HE C, et al.Changes of fresh chimonobambusa dietary fiber in properties and structure modified by microwave and fine grinding[J].Food and Fermentation Industries, 2017, 43(8):145-150.
[31] EOM T, KIM Y S, CHOI C H, et al.Current understanding of microbiota-and dietary-therapies for treating inflammatory bowel disease[J].Journal of Microbiology, 2018, 56(3):189-198.
[32] WEN Y, NIU M, ZHANG B J, et al.Structural characteristics and functional properties of rice bran dietary fiber modified by enzymatic and enzyme-micronization treatments[J].LWT-Food Science and Technology, 2017, 75:344-351.
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