食品与发酵工业 >

2025 , Vol. 51 >Issue 16: 182 - 189

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.040607

研究报告

发酵麸皮对葡聚糖硫酸钠诱导小鼠溃疡性结肠炎的保护作用

  • 邓娟丽 ,
  • 杜稳 ,
  • 詹清宇 ,
  • 李一帆 ,
  • 孙长坡 ,
  • 李天天
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  • 1(河南工业大学 粮油食品学院,河南 郑州,450001)
    2(国家粮食和物资储备局科学研究院,粮油加工研究所,北京,100037)
    3(中国农业大学 资源与环境学院,北京,100091)
    4(北京农学院 文法与城乡发展学院,北京,102206)
    5(国家粮食和物资储备局标准质量中心,北京,100834)
第一作者:硕士(孙长坡研究员和李天天助理研究员为共同通信作者,E-mail:scp@ags.ac.cn;ltt@ags.ac.cn)

收稿日期: 2024-07-30

  修回日期: 2024-12-04

  网络出版日期: 2025-08-29

基金资助

中央级公益性科研院所基本科研业务费专项项目(ZX2414)

收起

Protective effects of fermented wheat bran on dextran sodium sulfate-induced ulcerative colitis in mice

  • DENG Juanli ,
  • DU Wen ,
  • ZHAN Qingyu ,
  • LI Yifan ,
  • SUN Changpo ,
  • LI Tiantian
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  • 1(College of Food Science and Engineering, Henan University of Technology, Zhengzhou 450001 China)
    2(Institute of Cereal and Oil Science and Technology, Academy of National Food and Strategic Reserves Administration, Beijing 100037, China)
    3(College of Resources and Environmental Sciences, China Agricultural University, Beijing 100091, China)
    4(College of Humanities and Urban-Rural Development, Beijing University of Agriculture, Beijing 102206, China)
    5(Standard Quality Center of National Food and Strategic Reserves Administration, Beijing 100834, China)

Received date: 2024-07-30

  Revised date: 2024-12-04

  Online published: 2025-08-29

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摘要

该研究采用3%(质量分数)葡聚糖硫酸钠(dextran sodium sulfate,DSS)诱导建立溃疡性结肠炎(ulcerative colitis, UC)小鼠模型,探究发酵麸皮(fermented wheat bran,FWB)对溃疡性结肠炎的保护作用及对肠道菌群的影响。将12周龄的雌性BALB/c小鼠随机分为对照组、模型组、发酵麸皮组、发酵麸皮+模型组,持续饲喂14 d。记录小鼠体重以及采食量;检测小鼠结肠组织病理、血浆炎症因子和肠道菌群的变化。与模型组相比,发酵麸皮能够有效缓解小鼠溃疡性结肠炎的症状,减轻结肠组织病理损伤;抑制炎症因子白细胞介素(interleukin,IL)-1β(P＜0.01)和肿瘤坏死因子(tumor necrosis factor,TNF)(P＜0.01)的表达。粪便16S rRNA测序结果显示,发酵麸皮改善了由溃疡性结肠炎引起的肠道菌群失调,增加了肠道菌群的多样性;肠道微生物和炎症细胞因子关联性分析表明,溃疡性结肠炎小鼠血浆IL-1β、TNF-α、IL-6含量与埃希氏-志贺氏菌属、罗姆布茨菌属等丰度相关性显著。综上所述,发酵麸皮可以减轻溃疡性结肠炎小鼠的症状和降低促炎细胞因子水平,其机制与调节肠道菌群、抑制有害菌和促进有益菌生长有关。

关键词： 发酵麸皮; 溃疡性结肠炎; 肠道菌群; 炎症因子; 保护作用

本文引用格式

邓娟丽 , 杜稳 , 詹清宇 , 李一帆 , 孙长坡 , 李天天 . 发酵麸皮对葡聚糖硫酸钠诱导小鼠溃疡性结肠炎的保护作用[J]. 食品与发酵工业, 2025 , 51(16) : 182 -189 . DOI: 10.13995/j.cnki.11-1802/ts.040607

Abstract

In this study, 3% dextran sodium sulfate (DSS) was used to establish ulcerative colitis (UC) mouse model to investigate the protective effect of fermented wheat bran (FWB) on ulcerative colitis and its impact on the gut microbiota.Twelve-week-old female BALB/c mice were randomly divided into control, DSS, FWB, and FWB+DSS groups for 14 days.Body weights and feed intake were recorded, and changes in colonic histopathology, plasma inflammatory factors, and intestinal flora were detected.Compared with the DSS group, FWB could effectively alleviate the symptoms of ulcerative colitis and reduce the pathological damage to colon tissue and it also inhibited the expression of inflammatory factors IL-1β (P＜0.01) and TNF-α (P＜0.01).Fecal 16S rRNA sequencing results showed that FWB improved the dysbiosis of the gut microbiota caused by ulcerative colitis and increased the diversity of the gut microbiota and the correlation analysis between gut microbiota and inflammatory cytokines indicated that the levels of IL-1β, TNF-α, and IL-6 in the plasma of ulcerative colitis mice were significantly correlated with the abundance of Escherichia-Shigella and Romboutsia.In conclusion, FWB can alleviate the symptoms of ulcerative colitis in mice and reduce the levels of pro-inflammatory cytokines, and its mechanism is related to the regulation of gut microbiota, inhibition of harmful bacteria, and promotion of the growth of beneficial bacteria.

Key words： fermented wheat bran; ulcerative colitis; intestinal flora; inflammatory factors; protective effects

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