食品与发酵工业 >

2025 , Vol. 51 >Issue 16: 305 - 313

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.041579

分析与检测

胡柚蜂蜜主要理化指标及标志性成分分析

  • 刘西子 ,
  • 齐宁馨 ,
  • 李泓飞 ,
  • 赵文 ,
  • 张琳 ,
  • 刘春福 ,
  • 胡菡
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  • 1(中国农业科学院蜜蜂研究所,资源昆虫高效养殖与利用全国重点实验室,北京,100093)
    2(浙江花谷源蜂业有限公司,浙江 衢州,324003)
第一作者:硕士研究生(胡菡副研究员为通信作者,E-mail:huhan@caas.cn)

收稿日期: 2024-11-14

  修回日期: 2025-01-23

  网络出版日期: 2025-08-29

基金资助

国家重点研发计划项目(2022YFF0606805)

收起

Main quality and characteristic components analysis of Huyou honey

  • LIU Xizi ,
  • QI Ningxin ,
  • LI Hongfei ,
  • ZHAO Wen ,
  • ZHANG Lin ,
  • LIU Chunfu ,
  • HU Han
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  • 1(State Key Laboratory of Resource Insects, Bee Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100093, China)
    2(Zhejiang Huaguyuan Bee Industry Co.Ltd., Quzhou 324003, China)

Received date: 2024-11-14

  Revised date: 2025-01-23

  Online published: 2025-08-29

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摘要

该研究对胡柚蜂蜜的孢粉学特征、水分含量、糖分、淀粉酶活性等理化参数进行分析,检测结果表明,胡柚蜂蜜是一种各项基本理化指标均符合蜂蜜国家标准的优质蜂蜜。同时,采用超高效液相色谱-串联质谱法对胡柚蜂蜜和其他4种中国常见蜂蜜进行了非靶向化合物成分分析,结合主成分分析和正交偏最小二乘判别分析模型筛选可用于胡柚蜂蜜植物源鉴别的标志性成分[变量投影重要性(variable imprtance in projection,VIP)>1,P<0.01,倍性变化(fold change,FC)>2],共发现22种差异性化合物,其中新橙皮苷(含量约为0.961~1.478 mg/kg)和柠檬苦素(含量约为0.347~0.659 mg/kg)是胡柚蜂蜜中所特有且表达量较高的2种化合物,可用于区分胡柚蜂蜜与其他蜂蜜。该研究为胡柚蜂蜜的品质评价和种类鉴别提供理论参考。

关键词： 胡柚蜂蜜; 标志性成分; 种类鉴别; 新橙皮苷; 柠檬苦素

本文引用格式

刘西子 , 齐宁馨 , 李泓飞 , 赵文 , 张琳 , 刘春福 , 胡菡 . 胡柚蜂蜜主要理化指标及标志性成分分析[J]. 食品与发酵工业, 2025 , 51(16) : 305 -313 . DOI: 10.13995/j.cnki.11-1802/ts.041579

Abstract

To clarify the main quality and characteristic components of Huyou honey, key physicochemical parameters, such as water content, sugar content, and amylase activity, were analyzed in this present study.Results showed that Huyou honey was a kind of high-quality honey that basic physicochemical indexes comply with the national standard of honey.Meanwhile, the non-targeted metabolomics strategy based on ultra performance liquid chromatography-tandem high resolution mass spectrometer (UPLC-HRMS) was utilized to comprehensively analyze diverse metabolic compounds of Huyou honey and other four common Chinese honeys.The principal component analysis (PCA) and volcano plotting and orthogonal partial least-squares discriminant analysis (OPLS-DA) were employed to evaluate the difference in different honeys.Using the criteria of VIP>1,P<0.01,and FC>2, total 22 components with significant differences were identified.Among them, neohesperidin and limonin were identified as two reliable chemical markers for Huyou honey authentication, which were absent in other honeys.Furthermore, a full-scan mass spectrometry-parallel reaction monitoring (FullMS-PRM) method was developed to determine these two characteristic components in honey samples.In Huyou honey, the contents of neohesperidin and limonin were ranging from 0.961 to 1.478 mg/kg and from 0.347 to 0.659 mg/kg, respectively.This study is the first systematic research on the authenticity evaluation of Huyou honey, which provides an important theoretical basis for the establishment of quality standards for Huyou honey.

Key words： Huyou honey; characteristic components; identification of species; neohesperidin; limonin

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