阿胶与阿胶糕作为功能食品的开发具有广阔前景。基于功能成分表征、体外抗氧化和细胞实验的角度,评价阿胶和阿胶糕不同提取物抗皮肤衰老的功效。结果表明,体积分数为70%乙醇溶液相比水、正己烷、乙酸乙酯,最有利于阿胶糕中多酚类物质的溶出。相比阿胶醇提物,阿胶糕醇提物具有更高占比的抗氧化活性氨基酸和更小分子质量的肽段。抗氧化实验表明,阿胶和阿胶糕醇提物相比,其他提取物表现出更强的ABTS阳离子自由基、DPPH自由基、羟自由基以及超氧阴离子自由基清除作用。细胞活力实验表明,25~400 μg/mL质量浓度的阿胶和阿胶糕提取物对细胞活力没有毒害作用。利用H2O2诱导氧化应激构建细胞衰老模型,发现200 μg/mL阿胶和阿胶糕醇提物作用下,损伤HaCaT细胞内活性氧相比诱导组分别降低(28.41±2.06)%和(41.83±2.81)%,分别达到(84.16±3.21)%和(90.99±3.14)%的细胞存活率,表现出对损伤细胞的保护作用。该研究结果为阿胶和阿胶糕应用于抗细胞衰老的研究提供了一定的科学依据。
Developing Colla Corii Asini and Ejiao cake as a health food has broad prospects.Based on the characterization of functional components, in vitro antioxidants, and cell experiments, the anti-skin aging effects of different polar solvent extracts of Colla Corii Asini and Ejiao cake were evaluated.Firstly, compared with other solvents (water, n-hexane, ethyl acetate), 70% ethanol solution was the most conducive to the dissolution of functional active ingredients such as sesamin, sesamol, and sesamin melanin in Ejiao cake.The peptides with a molecular weight of less than 500 Da accounted for 15.39% and 36.43% of the total peptides in the ethanol extracts of Colla Corii Asini and Ejiao cake, respectively.Compared with the ethanol extracts of Colla Corii Asini, the ethanol extracts of Ejiao cake had a higher proportion of antioxidant-active amino acids, such as glutamic acid, proline, and alanine.Secondly, antioxidant experiments showed that the ethanol extracts of Colla Corii Asini and Ejiao cake showed stronger ABTS cationic radical, DPPH free radical, hydroxyl radical, and superoxide anion scavenging effects than other extracts.Moreover, cell viability experiments showed that different extracts of Colla Corii Asini and Ejiao cake had no toxic effect on cell viability at the concentration range of 25-400 μg/mL.The cell senescence model was constructed by H2O2-induced oxidative stress.It was found that under the action of 200 μg/mL ethanol extract of Colla Corii Asini and Ejiao cake, the highest survival rates of damaged HaCaT cells were (84.16±3.21)% and (90.99±3.14)%, respectively.Thus, ethanol extract from Colla Corii Asini and Ejiao cake exhibited protective effects on the damaged cells.The scavenging rates of 200 μg/mL ethanol extract of Colla Corii Asini and Ejiao cake on reactive oxygen free radicals were (28.41±2.06)% and (41.83±2.81)% in damaged HaCaT cells, respectively.The results of this study provide a scientific basis for the application of Colla Corii Asini and Ejiao cake in the study of anti-cell senescence.
[1] 孙睿. 寡肽类化合物抗皮肤衰老的机制研究[D].济南:齐鲁工业大学,2023.
SUN R.Study on the mechanism of oligopeptide compounds against skin aging[D].Jinan:Qilu University of Technology, 2023.
[2] 王培宇.南瓜籽多肽制备及其抗衰老作用研究[D].无锡:江南大学,2020.
WANG P Y.Study on preparation of pumpkin seed peptides and their anti-aging effect[D].Wuxi:Jiangnan University, 2020.
[3] 陈舜让, 付云芸, 陈泳斯, 等.铁皮石斛多糖对H2O2损伤HaCaT细胞的保护作用[J].今日药学, 2021, 31(6):429-432.
CHEN S R, FU Y Y, CHEN Y S, et al.The protection effect of Dendrobium officinale polysaccharides on H2O2-induced oxidative stress in HaCaT cells[J].Pharmacy Today, 2021, 31(6):429-432.
[4] 陈慧, 彭雨欣, 徐莹皓, 等.SIRT3多酚激活剂的抗细胞氧化应激作用及机制[J].中国食品学报, 2022, 22(1):12-20.
CHEN H, PENG Y X, XU Y H, et al.Inhibitive effects of SIRT3-augmenting polyphenols on cellular oxidative stress[J].Journal of Chinese Institute of Food Science and Technology, 2022, 22(1):12-20.
[5] 石浩, 王仁才, 吴小燕, 等.软枣猕猴桃黄酮对过氧化氢诱导HaCaT细胞损伤的保护作用[J].食品科学, 2018, 39(13):229-234.
SHI H, WANG R C, WU X Y, et al.Protective effect of Actinidia arguta flavonoid on hydrogen peroxide-induced injury in HaCaT cells[J].Food Science, 2018, 39(13):229-234.
[6] 樊雨梅, 汝文文, 史传超, 等.阿胶低聚肽的成分分析及其抗氧化活性[J].食品工业科技, 2020, 41(18):314-318; 323.
FAN Y M, RU W W, SHI C C, et al.Composition and its antioxidant activity of Colla corii asini oligopeptides[J].Science and Technology of Food Industry, 2020, 41(18):314-318; 323.
[7] 杜博玮, 徐晓冰, 郭尚伟, 等.高抗氧化性小分子阿胶的研究[J].北京化工大学学报(自然科学版), 2019, 46(6):15-20.
DU B W, XU X B, GUO S W, et al.Preparation of a high-antioxidant hydrolysate from Colla corii asini[J].Journal of Beijing University of Chemical Technology (Natural Science Edition), 2019, 46(6):15-20.
[8] 曹菲薇. 阿胶糕及其主要原料阿胶的抗氧化作用及机理研究[D].杭州:浙江大学,2019.
CAO F W.Antioxidant effects and its mechanism of Ejiao cake and its main raw material Colla Corii Asini[D].Hangzhou:Zhejiang University, 2019.
[9] 罗自舒. 黑芝麻特征营养成分分析及VE含量候选基因鉴定[D].北京:中国农业科学院,2023.
LUO Z S.Analysis of characteristic nutrients of black sesame and identification of candidate genes for VE content[D].Beijing:Chinese academy of agricultural sciences, 2023.
[10] 李杰, 贾栩超, 张瑞芬, 等.黑芝麻黑色素的分离纯化、结构表征及体外抗氧化活性[J].中国农业科学, 2020, 53(12):2477-2492.
LI J, JIA X C, ZHANG R F, et al.Isolation, structural characterization and antioxidant activity of black sesame melanin[J].Scientia Agricultura Sinica, 2020, 53(12):2477-2492.
[11] 曲媛鑫, 付英杰.阿胶化学成分、质量控制及药理作用研究进展[J].特产研究, 2023, 45(3):136-143.
QU Y X, FU Y J.Advances in chemical constituents, quality control and pharmacological effects of asini corii Colla[J].Special Wild Economic Animal and Plant Research, 2023, 45(3):136-143.
[12] 姜一朴, 邸志权, 王延涛, 等.小分子阿胶抗疲劳、抗氧化及止血作用研究[J].中国药理学通报, 2019, 35(2):203-208.
JIANG Y P, DI Z Q, WANG Y T, et al.Study on anti-fatigue, anti-oxidative and hemostatic effects of small molecule Asini Corii Colla[J].Chinese Pharmacological Bulletin, 2019, 35(2):203-208.
[13] 宋亚静, 林前锦, 吴妍欣, 等.茭白提取物及不同溶剂萃取物的抗氧化活性研究[J].粮食与油脂, 2023, 36(11):138-143.
SONG Y J, LIN Q J, WU Y X, et al.Study on the antioxidant activity of extracts of Zizania latifolia and extracts from different solvents[J].Cereals & Oils, 2023, 36(11):138-143.
[14] 张成栋, 樊晓俞, 李丽宁, 等.紫花亚菊不同溶剂提取物对肿瘤细胞活性的影响[J].西藏医药, 2024,45(1):152-154.
ZHANG C D, FAN X Y, LI L N, et al.Effects of different solvent extracts from Echinacea purpurea on tumor cell activity[J].Tibetan Medical Journal, 2024,45(1):152-154.
[15] 于群. 芦笋多酚结合热加工抑制酪氨酸酶活性的研究[D].无锡:江南大学,2021.
YU Q.Study on asparagus polyphenols combined with thermal processing to inhibit tyrosinase activity[D].Wuxi:Jiangnan University, 2021.
[16] 苏小明, 王岳杨, 徐铭晨, 等.五味子丙素对H2O2诱导的HaCaT细胞衰老作用的影响及机制[J].解放军医学杂志, 2021, 46(3):221-230.
SU X M, WANG Y Y, XU M C, et al.Anti-aging effects of schisandrin C on H2O2-induced HaCaT[J].Medical Journal of Chinese PLA, 2021, 46(3):221-230.
[17] 刘润华. 芝麻提取物对高脂模型诱导的小鼠肥胖的干预作用及机制研究[D].杨凌:西北农林科技大学,2018.
LIU R H.Study on the intervention effect and mechanism of sesame extract on obesity induced by high fat model in mice[D].Yangling:Northwest A&F University, 2018.
[18] MENDIS E, RAJAPAKSE N, BYUN H G, et al.Investigation of jumbo squid (Dosidicus gigas) skin gelatin peptides for their in vitro antioxidant effects[J].Life Sciences, 2005, 77(17):2166-2178.
[19] 郑琳. 抗氧化肽的构效关系及定向制备的研究[D].广州:华南理工大学,2015.
ZHENG L.Study on the structure-activity relationship and directional preparation of antioxidant peptides[D].Guangzhou:South China University of Technology, 2015.
