食品与发酵工业 >

2025 , Vol. 51 >Issue 17: 128 - 134

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.041309

研究报告

紫苏桑叶金银花固体饮料对小鼠的免疫调节及抗突变作用

  • 武新月 ,
  • 张薇 ,
  • 俞萍 ,
  • 张颖 ,
  • 赵悦 ,
  • 梁婕
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  • (江苏省疾病预防控制中心,江苏 南京,210009)
第一作者:硕士,主管技师(梁婕主任医师为通信作者,E-mail:liangj@jscdc.cn)

收稿日期: 2024-10-15

  修回日期: 2024-12-25

  网络出版日期: 2025-09-29

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Immunomodulatory and anti-mutational effects of perilla and mulberry leaf and honeysuckle solid beverage in mice

  • WU Xinyue ,
  • ZHANG Wei ,
  • YU Ping ,
  • ZHANG Ying ,
  • ZHAO Yue ,
  • LIANG Jie
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  • (Jiangsu Provincial Center for Disease Control and Prevention, Nanjing 210009, China)

Received date: 2024-10-15

  Revised date: 2024-12-25

  Online published: 2025-09-29

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摘要

为了研究紫苏桑叶金银花固体饮料对小鼠免疫功能的影响,通过环磷酰胺诱导建立免疫低下小鼠模型,设立空白组、模型组、固体饮料低(833 mg/kg·BW)、中(1 667 mg/kg·BW)、高(5 000 mg/kg·BW)剂量组,测定各组小鼠免疫学指标。结果显示,高剂量组小鼠的胸腺指数和脾脏指数均显著上升;各剂量组小鼠血液中红细胞和血红蛋白的数目均显著升高,高剂量组血小板和红细胞压积数目显著升高;高剂量组小鼠血清中TNF-α、INF-γ和IL-1β的水平均显著下调。为了研究固体饮料的抗突变作用,进行Ames试验、骨髓微核试验和睾丸染色体畸变试验。结果显示,固体饮料对2-氨基芴诱导的Ames菌株回复突变具有拮抗作用;各剂量组小鼠骨髓细胞微核率均显著下降,雌性小鼠低、中、高剂量组的微核抑制率分别为30.4%、46.4%和60.5%,雄性小鼠低、中、高剂量组的微核抑制率分别为30.5%、46.9%和59.1%;中、高剂量组小鼠睾丸精母细胞染色体畸变率均显著下降,低、中、高剂量组的抑制率分别为8.9%、25.9%和65.2%。以上结果表明,紫苏桑叶金银花固体饮料对小鼠具有免疫调节以及抗突变作用。

关键词： 紫苏提取物; 固体饮料; 环磷酰胺; 免疫功能; 抗突变

本文引用格式

武新月 , 张薇 , 俞萍 , 张颖 , 赵悦 , 梁婕 . 紫苏桑叶金银花固体饮料对小鼠的免疫调节及抗突变作用[J]. 食品与发酵工业, 2025 , 51(17) : 128 -134 . DOI: 10.13995/j.cnki.11-1802/ts.041309

Abstract

To study the effects of perilla and mulberry leaf and honeysuckle solid beverage on immune function, an immunosuppressed mouse model that was constructed using cyclophosphamide was investigated, mice were randomly divided into blank control group, model group and low (833 mg/kg·BW), medium (1 667 mg/kg·BW) and high (5 000 mg/kg·BW) dose solid beverage groups, immunological indices were measured.The results showed that the thymus index and spleen index of high dose group significantly increased;The number of RBC and HGB significantly increased in each dose group, while the number of PLT and HCT in the high-dose group significantly increased;The levels of TNF-α, INF-γ, and IL-1β in serum of high dose group were significantly downregulated.To study the antimutagenic effects of solid beverages, the Ames test, mouse bone marrow erythrocyte micronucleus test, and mouse spermatocyte chromosome aberration test were selected.The results showed that solid beverage had an antagonistic effect on 2-aminofluorene-induced back mutation of Ames strain, the micronucleus rate of bone marrow cells significantly decreased in each dose group, the micronucleus inhibition rate of female mice in low, medium and high dose groups were 30.4%, 46.4%, and 60.5%, respectively;the micronucleus inhibition rate of male mice in low, medium and high dose groups were 30.5%, 46.9%, and 59.1%, respectively;the chromosome aberration rate of testicular spermatocyte in medium and high dose groups decreased significantly, and the inhibition rate in low, medium and high dose groups was 8.9%, 25.9%, and 65.2%, respectively.In conclusion, perilla mulberry leaf and honeysuckle solid beverage have immunomodulatory and antimutagenic effects on mice.

Key words： perilla extract; solid beverage; cyclophosphamide; immune function; antimutation

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