采后果实抗坏血酸(ascorbic acid,AsA)包含氧化态和还原态2种存在形式,贮藏过程中AsA含量的下降是影响果实品质的重要因素。为探究2,4-表油菜素内酯(2,4-epibrassinolide,EBR)对采后草莓AsA代谢的调控机制,研究采用5 μmol/L EBR对采后草莓果实进行喷洒处理,并在(20±1) ℃、90%~95%相对湿度条件下进行为期5 d的贮藏实验。结果表明,EBR处理显著抑制了还原态AsA、氧化态AsA[脱氢抗坏血酸(dehydroascorbic acid,DHA)]及总AsA(AsA+DHA)含量的下降,同时维持了AsA/DHA比值的平衡。通过转录组学分析,发现EBR处理共调控了3 309个差异表达基因,其中1 491个基因上调,1 818个基因下调。进一步结合实时荧光定量PCR(quantitative real-time polymerase chain reaction,qRT-PCR)技术,明确了EBR处理后草莓果实AsA合成代谢相关基因的转录变化,发现FaMIOX和FaAPX基因的上调表达对AsA/DHA平衡具有积极作用,且其表达可能受WRKY家族转录因子的调控。研究揭示了EBR通过调控关键基因表达延缓采后草莓AsA含量下降的分子机制,为EBR在采后果实保鲜中的应用提供了理论依据和实践参考。
Postharvest fruit ascorbic acid (AsA) exists in both oxidized and reduced forms and the decline of AsA content during postharvest storage is a critical factor affecting fruit quality.To investigate the regulatory mechanism of 2,4-epibrassinolide (EBR) on AsA metabolism in postharvest strawberries, this study applied 5 μmol/L of EBR to strawberry fruit and conducted a 5-day storage experiment at (20±1) ℃ with 90%-95% relative humidity.Results showed that EBR treatment significantly inhibited the decline in reduced AsA, dehydroascorbic acid (DHA) and total AsA contents, while maintaining the AsA/DHA ratio.Transcriptome analysis revealed that EBR treatment regulated 3 309 differentially expressed genes, including 1 491 upregulated and 1 818 downregulated genes.Further analysis using quantitative real-time PCR (qRT-PCR) clarified the transcriptional changes in AsA metabolic pathway-related genes in EBR-treated strawberry fruits.Notably, the upregulated expression of FaMIOX and FaAPX genes positively contributed to AsA/DHA homeostasis, potentially regulated by WRKY family transcription factors.This study elucidates the molecular mechanism by which EBR delays AsA degradation in postharvest strawberries through the regulation of key gene expression, providing a theoretical basis and practical reference for the application of EBR in postharvest fruit preservation.
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