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CRISPR/Cas9基因编辑技术及其在酵母菌中的应用研究进展

  • 泽仁德西 ,
  • 徐红梅 ,
  • 熊德文 ,
  • 刘超逸 ,
  • 刘文青 ,
  • 杭方学 ,
  • 王淑培
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  • 1(广西大学 轻工与食品工程学院,广西 南宁,530004)
    2(南宁师范大学 环境与生命科学学院,广西 南宁,530001)
第一作者:硕士研究生(王淑培副教授为通信作者,E-mail:wshp2000@163.com)

收稿日期: 2024-09-12

  修回日期: 2024-12-23

  网络出版日期: 2025-09-29

基金资助

国家自然科学基金青年基金项目(32302164);广西自然科学基金青年基金项目(2022GXNSFBA035561)

Research progress of CRISPR/Cas9 gene editing technology and its application in yeast

  • ZEREN Dexi ,
  • XU Hongmei ,
  • XIONG Dewen ,
  • LIU Chaoyi ,
  • LIU Wenqing ,
  • HANG Fangxue ,
  • WANG Shupei
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  • 1(School of Light Industry and Food Engineering, Guangxi University, Nanning 530004, China)
    2(School of Environmental and Life Sciences, Nanning Normal University, Nanning 530001, China)

Received date: 2024-09-12

  Revised date: 2024-12-23

  Online published: 2025-09-29

摘要

CRISPR/Cas系统是细菌和古生菌中发现的自适应防御系统,其中CRISPR/Cas9因其高效、特异以及简便的优势,已在动物、植物和微生物基因功能研究和遗传改良中得到广泛应用。该文综述了CRISPR/Cas系统的历史、结构、分类情况及CRISPR/Cas9的作用机理,归纳总结了CRISPR/Cas9系统在模式酵母菌和非模式酵母菌中的应用及其衍生系统在酵母菌中的应用,并展望了该技术的应用前景,以期为更多种类酵母菌开展基因编辑提供参考。

本文引用格式

泽仁德西 , 徐红梅 , 熊德文 , 刘超逸 , 刘文青 , 杭方学 , 王淑培 . CRISPR/Cas9基因编辑技术及其在酵母菌中的应用研究进展[J]. 食品与发酵工业, 2025 , 51(17) : 367 -378 . DOI: 10.13995/j.cnki.11-1802/ts.041040

Abstract

The CRISPR/Cas system constitutes an adaptive defense mechanism present in bacteria and archaea.Among its variants, CRISPR/Cas9 has garnered extensive application in the study of gene function and genetic enhancement across animal, plant, and microbial domains, owing to its high efficiency, specificity, and simplicity.This review provides a comprehensive examination of the historical background, structural features, and classification criteria of the CRISPR/Cas system, while elucidating the operational mechanisms of CRISPR/Cas9.Additionally, the paper also summarizes the diverse applications of the CRISPR/Cas9 system in both model and non-model yeasts, as well as their derivative systems.The review also offers insights into future prospects for this technology, with the aim of facilitating gene editing across a broader spectrum of yeast species.

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