食品与发酵工业 >

2025 , Vol. 51 >Issue 18: 295 - 303

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.041495

研究报告

灰树花发酵液对UVB诱导人永生化角质形成细胞的修复作用

  • 方忆凡 ,
  • 方嘉璇 ,
  • 宋子欣 ,
  • 赵亿 ,
  • 史豆豆 ,
  • 李萌 ,
  • 王冬冬 ,
  • 王昌涛
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  • 1(北京工商大学 轻工科学与工程学院,北京,100048)
    2(北商加美(北京)科技有限公司,北京,100048)
第一作者:硕士研究生(王昌涛教授为通信作者,E-mail:wangct@th.btbu.edu.cn)

收稿日期: 2024-11-04

  修回日期: 2025-01-15

  网络出版日期: 2025-10-16

收起

Repairing effect of Grifolia grifolia fermentation broth on UVB-damaged human immortalized keratinocytes

  • FANG Yifan ,
  • FANG Jiaxuan ,
  • SONG Zixin ,
  • ZHAO Yi ,
  • SHI Doudou ,
  • LI Meng ,
  • WANG Dongdong ,
  • WANG Changtao
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  • 1(School of Light Industry Science and Engineering, Beijing Technology and Business University, Beijing 100048, China)
    2(Beishang Jiamei (Beijing) Technology Co.Ltd., Beijing 100048, China)

Received date: 2024-11-04

  Revised date: 2025-01-15

  Online published: 2025-10-16

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摘要

为提高灰树花子实体的利用率,该研究采用裂褶菌对灰树花子实体进行发酵,得到灰树花发酵液(Grifola frondosa fermentation broth, GFF),以未发酵液(unfermented Grifola frondosa solution, GF)作为对照,探究发酵对灰树花子实体中活性物质含量和功效的影响。通过建立UVB损伤模型,检测GF和GFF对UVB诱导的人永生化角质形成细胞(human immortalized keratinocytes, HaCaT)的存活率、抗氧化酶活性、活性氧(reactive oxygen species, ROS)含量、炎症因子表达量、屏障相关蛋白分泌情况及信号通路的影响。结果表明,GFF中活性物质含量及体外抗氧化活性均高于GF。GF和GFF可能通过抑制信号通路的表达,修复UVB诱导造成的HaCaT细胞损伤。GFF在提高细胞内抗氧化酶含量、促进屏障相关蛋白的表达、抑制ROS的生成及炎症因子分泌的效果均优于GF。红细胞溶血实验表明,GF和GFF均具有良好的安全性。通过裂褶菌发酵的灰树花子实体具有更强的抗氧化、抗炎和皮肤屏障修复的能力。

关键词： 灰树花发酵液; 抗氧化; 抗炎; 皮肤屏障; UVB

本文引用格式

方忆凡 , 方嘉璇 , 宋子欣 , 赵亿 , 史豆豆 , 李萌 , 王冬冬 , 王昌涛 . 灰树花发酵液对UVB诱导人永生化角质形成细胞的修复作用[J]. 食品与发酵工业, 2025 , 51(18) : 295 -303 . DOI: 10.13995/j.cnki.11-1802/ts.041495

Abstract

To enhance the utilization of Grifola frondosa seeds, this study investigated the fermentation of Grifola frondosa seeds using Schizophyllum commune.The unfermented Grifola frondosa solution (GF) served as a control to examine the effects of fermentation on the content and bioactivity of active compounds in the seeds.The impact of GF and the Grifola frondosa fermentation broth (GFF) on UVB-induced damage in human immortalized keratinocytes (HaCaT) was assessed by measuring cell survival, antioxidant enzyme activity, reactive oxygen species (ROS) levels, inflammatory marker expression, skin barrier-associated protein secretion, and relevant pathways.The results indicated that GFF contained higher levels of active substances and exhibited stronger antioxidant activity than GF.Both GF and GFF may repair UVB-induced damage in HaCaT by inhibiting the expression of the signaling pathway.The findings demonstrated that GFF exhibited superior efficacy compared to GF in enhancing antioxidant enzyme activity, promoting barrier protein expression, and reducing ROS production and inflammatory mediator secretion.Erythrocyte hemolysis experiments confirmed that both GF and GFF are safe for use.The Grifola frondosa seeds fermented with Schizophyllum commune possess enhanced antioxidant, anti-inflammatory, and skin barrier repair capabilities.

Key words： Grifola frondosa fermentation broth; antioxidant; anti-inflammatory; skin barrier; UVB

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