该研究采用高脂饲料诱导建立高血糖小鼠模型,探究青稞复配茶对高血糖小鼠的降血糖作用及对肝组织的保护作用。将6周龄的雄性C57BL/6J小鼠随机分为空白对照组、模型组、阳性药物组及青稞复配茶低、中、高剂量组,持续造模9周后干预样品。记录各组小鼠体重变化、空腹血糖值、检测小鼠葡萄糖耐量、血清中血脂指标、炎症因子水平及肝脏抗氧化和脂代谢相关酶等含量,分析小鼠肝脏组织病理变化。与模型组相比,青稞复配茶高剂量组小鼠体重显著降低(P<0.05),甘油三酯、总胆固醇和低密度脂蛋白胆固醇水平显著降低(P<0.01);空腹血糖值显著降低(P<0.01);葡萄糖耐量水平显著增强(P<0.01);青稞复配茶各剂量组肝组织变性情况明显减轻。上述结果表明,青稞复配茶通过提高机体抗氧化能力、减轻炎症损伤、调节体内糖脂代谢,发挥了降血糖、降血脂及减肥的作用。
This study aimed to investigate the hypoglycemic effect and hepatoprotective role of highland barley blended tea in a high-fat diet-induced hyperglycemic mouse model.Six-week-old male C57BL/6J mice were randomly assigned to one of six groups:A blank control group, a model group, a positive drug group, and three groups receiving low, medium, and high doses of highland barley blended tea, respectively.Following a 9-week modeling period, the intervention with the test samples was initiated.The body weight changes and fasting blood glucose levels of the mice were recorded, and glucose tolerance, serum lipid profiles, inflammatory cytokine levels, and the content of antioxidant and lipid metabolism-related enzymes in the liver were measured.Additionally, pathological changes in the liver tissue were analyzed.A significant reduction in body weight was observed in the high-dose highland barley blended tea group compared to the model group (P<0.05).Additionally, a substantial decrease in triglyceride, total cholesterol, and low-density lipoprotein cholesterol levels was evident (P<0.01).Fasting blood glucose levels showed a significant decline (P<0.01), and glucose tolerance exhibited a marked improvement (P<0.01).Furthermore, the degree of liver tissue degeneration was notably alleviated in all barley blended tea dosage groups.The findings suggest that the hypoglycaemic, lipid-lowering, and weight-loss effects of barley blended tea are a result of enhanced antioxidant capacity, reduced inflammatory damage, and regulated glucose and lipid metabolism.
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