辅酶Q10作为一种内源性脂质抗氧化剂,其高分子质量、强疏水性及多晶型等性质导致其外源性补充受到了极大的限制。为解决辅酶Q10生物可接受率低的问题,该文通过改变固液脂质比例对辅酶Q10晶体进行调控,并以单脂肪酸甘油酯为乳化剂制备辅酶Q10纳米结构脂质载体,表征其理化性质,探究晶体调控对稳定性和生物可接受率的影响。结果表明,随着液态脂质中链甘油三酯的比例上升,脂质相结晶度降低,纳米结构脂质载体的物理稳定性显著增强(P＜0.05);其中,P1/M2以稳定性、释放特性以及晶体修饰等方面的优势,在模拟体外消化中辅酶Q10生物可接受率达到(72.1±2.5)%,显著高于其他脂质载体(P＜0.05)。综上,通过脂质相对辅酶Q10晶体生长的调控,有效提高了其生物可接受率,并为其他脂溶性活性成分功能产品的开发提供了理论依据和技术支撑。
As an endogenous lipid antioxidant, the properties of coenzyme Q10, such as its high molecular weight, strong hydrophobicity, and polycrystalline shape, lead to its exogenous supplementation being greatly restricted.To solve the problem of low bioaccessibility of coenzyme Q10, coenzyme Q10 nanostructured lipid carriers were prepared using monoacylglyceride as an emulsifier and regulating coenzyme Q10 crystals by adjusting the solid-liquid lipid ratio, their physicochemical properties were characterized and the effects of crystal modification on stability and bioaccessibility were investigated.Results showed that the physical stability of the nanostructured lipid carriers was significantly enhanced with the increase of the proportion of chain triglycerides in liquid lipids and the decrease of the crystallinity of the lipid phase (P<0.05), among them, P1/M2, with the advantages of stability, release characteristics, and crystal modification, achieved a bioaccessibility of coenzyme Q10 in simulated in vitro digestion of (72.1±2.5)%, which was significantly higher than that of other lipid carriers (P＜0.05).In conclusion, the regulation of coenzyme Q10 crystal growth through the lipid phase effectively improved its bioaccessibility and provided a theoretical basis and technical support for the development of functional products of other fat-soluble active ingredients.
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