食品与发酵工业 >

2025 , Vol. 51 >Issue 23: 219 - 226

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.042425

研究报告

基于超支化β-葡聚糖的新型补锌剂制备与表征

  • 李萃 ,
  • 温嘉怡 ,
  • 徐萌萌 ,
  • 石贵阳 ,
  • 陈磊 ,
  • 丁重阳
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  • 1(江南大学 生物工程学院,糖化学与生物技术教育部重点实验室,江苏 无锡,214122)
    2(江南大学,粮食发酵与食品生物制造国家工程研究中心,江苏 无锡,214122)
第一作者:硕士研究生(丁重阳教授和陈磊副教授为共同通信作者,E-mail:bioding@163.com;leichen@jiangnan.edu.cn)

收稿日期: 2025-02-19

  修回日期: 2025-04-17

  网络出版日期: 2025-12-25

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Preparation and characterization of a novel zinc supplement based on hyperbranched β-glucan

  • LI Cui ,
  • WEN Jiayi ,
  • XU Mengmeng ,
  • SHI Guiyang ,
  • CHEN Lei ,
  • DING Zhongyang
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  • 1(Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China)
    2(National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Wuxi 214122, China)

Received date: 2025-02-19

  Revised date: 2025-04-17

  Online published: 2025-12-25

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摘要

为开发具有高消化特性的新型补锌剂,该研究设计并构建了虎奶菇超支化β-葡聚糖-锌螯合体系(Pleurotus tuber-regium hyperbranched β-glucane-Zn,PTR-HBG-Zn),系统优化其制备工艺并评估其生物利用特性。通过单因素试验明确了关键工艺参数的影响规律:在pH 6.0、50 ℃及0.35 mol/L ZnSO4初始浓度条件下持续反应2 h,获得最高锌结合量为(47.06±1.85)mg/g的产物。采用傅里叶变换红外光谱、X-射线衍射等确认成功制备螯合物,揭示了锌配位诱导多糖分子链构象的转变。研究发现,锌离子的配位作用显著改变了体系流变特性,黏弹性模量数据及冷场发射扫描电子显微镜图像证实体系转化为典型凝胶态。体外模拟胃肠代谢研究表明,相较于无机锌(ZnSO4)和二代有机锌(葡萄糖酸锌),PTR-HBG-Zn在肠道环境下的锌溶解率显著提升(P<0.05)。因此,PTR-HBG-Zn螯合物具有更好的生物利用率和稳定性,为开发新型多糖补锌剂提供了理论依据和数据支持。

关键词： 超支化β-葡聚糖; 多糖-Zn; 补锌剂; 微量元素; 生物利用率

本文引用格式

李萃 , 温嘉怡 , 徐萌萌 , 石贵阳 , 陈磊 , 丁重阳 . 基于超支化β-葡聚糖的新型补锌剂制备与表征[J]. 食品与发酵工业, 2025 , 51(23) : 219 -226 . DOI: 10.13995/j.cnki.11-1802/ts.042425

Abstract

To develop a novel zinc-fortifying agent with enhanced digestibility and absorption, this study designed a hyperbranched polysaccharide-zinc chelate (PTR-HBG-Zn) using Pleurotus tuber-regium hyperbranched β-glucan (PTR-HBG) as the ligand.The preparation process was systematically optimized through single-factor experiments.The optimal conditions, including pH 6.0, 50 ℃, and 0.35 mol/L initial zinc sulfate concentration with 2 h reaction duration, yielded a chelate with a zinc content of (47.06±1.85) mg/g.Multidimensional characterization techniques including FTIR and XRD confirmed successful chelation and revealed conformational transitions in the polysaccharide chain conformation upon zinc binding.Rheological analysis and FESEM observations further demonstrated that zinc coordination induced gelation.In vitro simulated gastrointestinal digestion revealed that PTR-HBG-Zn exhibited significantly higher zinc dissolution rates (P<0.05) in the intestinal phase compared to zinc sulfate and zinc gluconate.These findings highlight the superior bioavailability and stability of PTR-HBG-Zn, providing both theoretical foundations and experimental evidence for developing advanced polysaccharide-based zinc supplements.

Key words： hyperbranched β-glucan; polysaccharide-Zn; Zn supplement; trace elements; bioavailability

参考文献

[1] GIBSON R S.Zinc deficiency and human health:Etiology, health consequences, and future solutions[J].Plant and Soil, 2012, 361(1-2):291-299.
[2] HAASE H, MOCCHEGIANI E, RINK L.Correlation between zinc status and immune function in the elderly[J].Biogerontology, 2006, 7(5-6):421-428.
[3] DUAN M P, LI T, LIU B, et al.Zinc nutrition and dietary zinc supplements[J].Critical Reviews in Food Science and Nutrition, 2023, 63(9):1277-1292.
[4] 史攀琪. 小米糠多肽-锌螯合物的制备、结构表征与稳定性研究[D].太原:山西师范大学, 2022.
SHI P Q.Study on preparation, structure characterization and stability of millet bran derived peptide-zinc chelate[D].Taiyuan:Shanxi Normal University, 2022.
[5] ZHANG Y, KHAN M Z H, YUAN T T, et al.Preparation and characterization of D.opposita Thunb polysaccharide-zinc inclusion complex and evaluation of anti-diabetic activities[J].International Journal of Biological Macromolecules, 2019, 121:1029-1036.
[6] FENG Y Y, WU Y Y, DUAN R, et al.Structural characterization and anti-inflammatory effects of Enteromorpha prolifera polysaccharide-Fe/Zn complexes[J].International Journal of Biological Macromolecules, 2023, 253:127166.
[7] 杜利月, 赵新如, 王飞.香菇多糖锌螯合物的制备及结构表征和体外抗氧化活性研究[J].食品安全质量检测学报, 2023, 14(4):290-298.
DU L Y, ZHAO X R, WANG F.Preparation, structure characterization and in vitro antioxidant activity of lentinan-znic chelate[J].Journal of Food Safety & Quality, 2023, 14(4):290-298.
[8] 金鑫, 孙雪琳, 刘虹拴, 等. 蛹虫草提取物改善糖尿病肾病的药效及代谢组学[J]. 生物加工过程, 2025, 23(1): 77-85.
JIN X, SUN X L, LIU H S, et al. Pharmacodynamic and metabolomic studies of Cordyceps militaris extract in improving diabetic nephropathy[J]. Chinese Journal of Bioprocess Engineering, 2025, 23(1): 77-85.
[9] CHEN L, GE M D, ZHU Y J, et al.Structure, bioactivity and applications of natural hyperbranched polysaccharides[J].Carbohydrate Polymers, 2019, 223:115076.
[10] CHEN L, XU W W, LIN S L, et al.Cell wall structure of mushroom Sclerotium (Pleurotus Tuber Regium):Part 1.Fractionation and characterization of soluble cell wall polysaccharides[J].Food Hydrocolloids, 2014, 36:189-195.
[11] LI X J, ZHOU J J, LIU C R, et al.Stable and biocompatible mushroom β-glucan modified gold nanorods for cancer photothermal therapy[J].Journal of Agricultural and Food Chemistry, 2017, 65(43):9529-9536.
[12] TIMM T G, COSTA T M, ALBERTON M D, et al.Mushroom β-glucans:Application and innovation for food industry and immunotherapy[J].Applied Microbiology and Biotechnology, 2023, 107(16):5035-5049.
[13] 杨秀峰. 玛咖多糖的提取及其金属复合物的制备、表征和生物活性研究[D].昆明:云南师范大学, 2021.
YANG X F.Extraction of polysaccharide from Maca and study on the preparation, and characterization and bioactivity of Maca polysaccharide metal complex[D].Kunming:Yunnan Normal University, 2021.
[14] 董淑君, 张禧庆, 刘旭龙, 等.南瓜皮多糖锌的制备及生物利用率研究[J].食品工业科技, 2022, 43(18):200-207.
DONG S J, ZHANG X Q, LIU X L, et al.Preparation and bioavailability of pumpkin skin polysaccharide-zinc complex[J].Science and Technology of Food Industry, 2022, 43(18):200-207.
[15] 富天昕. 绿豆多肽锌螯合物的制备和结构鉴定及生物利用率研究[D].大庆:黑龙江八一农垦大学, 2020.
FU T X.Preparation, structure identification and bioavailability of mungbean polypeptide zinc chelate[D].Daqing:Heilongjiang Bayi Agricultural University, 2020.
[16] 龙瑞阳. 毛木耳多糖提取、结构及其锌复合物制备与生物活性研究[D].广州:广州大学, 2023.
LONG R Y.Extraction, structure, preparation and biological activity of polysaccharides from Auricularia polytricha and their zinc compounds[D].Guangzhou:Guangzhou University, 2023.
[17] JIA Y N, LI N N, WANG Q R, et al.Effect of Fe (Ⅲ), Zn (Ⅱ), and Cr (Ⅲ) complexation on the physicochemical properties and bioactivities of corn silk polysaccharide[J].International Journal of Biological Macromolecules, 2021, 189:847-856.
[18] WANG B, XIAO S, CHEN X Y, et al.Structural characterisation, gastrointestinal digestion stability and transepithelial transport study of casein peptide-zinc chelate[J].International Journal of Food Science & Technology, 2022, 57(5):2770-2778.
[19] ZHANG M, ZHAO H, SHEN Y, et al.Preparation, characterization and antioxidant activity evaluation in vitro of Fritillaria ussuriensis polysaccharide-zinc complex[J].International Journal of Biological Macromolecules, 2020, 146:462-474.
[20] 张铭冉, 薛天睿, 吕彬斐, 等.卵黄高磷蛋白磷酸肽-锌螯合物的制备及其结构表征[J].食品与发酵工业,2025,51(9):230-238.
ZHANG M R, XUE T R, LYU B F, et al. Preparation and structural characterization of phosvtin phosphopeptide peptide-zinc chelation[J]. Food and Fermentation Industries, 2025,51(9):230-238.
[21] 涂玲飞, 陈迎丽, 李焱, 等.白及多糖锌的结构表征及其抗氧化活性评价[J].食品与发酵工业, 2024, 50(17):218-225;245.
TU L F, CHEN Y L, LI Y, et al.Structure characterization and antioxidant activity of Bletilla striata polysaccharide-zinc[J].Food and Fermentation Industries, 2024, 50(17):218-225;245.
[22] CAI W D, QIU W Y, DING Z C, et al.Conformational and rheological properties of a quaternary ammonium salt of curdlan[J].Food Chemistry, 2019, 280:130-138.
[23] WANG H L, KE L J, DING Y N, et al.Effect of calcium ions on rheological properties and structure of Lycium barbarum L.polysaccharide and its gelation mechanism[J].Food Hydrocolloids, 2022, 122:107079.
[24] 冷雨佳. 大豆肽锌螯合物的制备、螯合机理及其生物活性研究[D].郑州:河南工业大学, 2022.
LENG Y J.Study on preparation, chelation mechanism and biological activity of soybean peptide zinc chelates[D].Zhengzhou:Henan University of Technology, 2022.
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