综述与专题评论

食品安全危害物仿生印迹亲和分析研究进展

  • 李兆周 ,
  • 李亚娟 ,
  • 陶健 ,
  • 黎乐乐 ,
  • 唐嘉敏 ,
  • 谢亚芳 ,
  • 魏雪冰 ,
  • 高红丽 ,
  • 陈秀金 ,
  • 万宁波 ,
  • 李芳 ,
  • 袁云霞
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  • 1(河南科技大学 食品与生物工程学院,河南 洛阳,471000)
    2(河南省食品绿色加工与质量安全控制国际联合实验室,河南 洛阳,471000)
    3(河南省食品和盐业检验技术研究院,河南 郑州,450003)
    4(国家市场监督管理总局重点实验室(食品安全快速检测与智慧监管技术),河南 郑州,450003)
第一作者:博士,教授(高红丽副教授为通信作者,E-mail:ghl3488579@163.com)

收稿日期: 2025-03-17

  修回日期: 2025-05-18

  网络出版日期: 2025-12-25

基金资助

河南省科技攻关项目(242102411001);洛阳市公益性行业科研专项项目(2202021A);河南省优秀青年科学基金项(202300410121);国家自然科学基金项目(31701694,U1504330);河南科技大学横向技术开发委托项目(横20240139,横20230120)

Research progress on biomimetic imprinting affinity analysis of food safety hazards

  • LI Zhaozhou ,
  • LI Yajuan ,
  • TAO Jian ,
  • LI Lele ,
  • TANG Jiamin ,
  • XIE Yafang ,
  • WEI Xuebing ,
  • GAO Hongli ,
  • CHEN Xiujin ,
  • WAN Ningbo ,
  • LI Fang ,
  • YUAN Yunxia
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  • 1(College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471000, China)
    2(Henan International Joint Laboratory of Food Green Processing and Quality Safety Control, Luoyang 471000, China)
    3(Henan Institute of Food and Salt Industry Inspection Technology, Zhengzhou 450003, China)
    4(Key Laboratory of Rapid Detection and Smart Supervision Technology for Food Safety, State Administration for Market Regulation, Zhengzhou 450003, China)

Received date: 2025-03-17

  Revised date: 2025-05-18

  Online published: 2025-12-25

摘要

食品安全危害物残留对人体健康的影响不容忽视,开发有效的检测技术尤为重要。仿生印迹技术是模拟抗体-抗原或者酶-底物的生物识别机制,能够对印迹分子进行专一识别。通过制备仿生印迹聚合物,结合高特异性亲和分析方法所构建的仿生印迹亲和分析技术具有快速、高效、灵敏以及低成本等优势。该文介绍了仿生印迹亲和分析识别元件的制备技术、表征方法以及相关应用的特点,总结了近年来荧光标记仿生印迹亲和分析、酶标记仿生印迹亲和分析、电化学仿生印迹亲和分析和仿生印迹化学发光亲和分析等不同分析法在食品危害物分析中的研究现状和应用进展。相关内容为食品安全检测新方法的研究提供新思路,为食品中危害物的检测提供技术支撑。

本文引用格式

李兆周 , 李亚娟 , 陶健 , 黎乐乐 , 唐嘉敏 , 谢亚芳 , 魏雪冰 , 高红丽 , 陈秀金 , 万宁波 , 李芳 , 袁云霞 . 食品安全危害物仿生印迹亲和分析研究进展[J]. 食品与发酵工业, 2025 , 51(23) : 424 -439 . DOI: 10.13995/j.cnki.11-1802/ts.042751

Abstract

The effects of residues of food safety hazards on human health should not be ignored, so it is particularly important to develop effective detection technology.Biomimetic imprinting is a biometric mechanism that simulates antibody-antigen or enzyme-substrate, and can identify imprinted molecules specifically.The biomimetic imprinting affinity analysis technology based on the preparation of biomimetic imprinting polymer combined with the high specificity affinity analysis method has the advantages of fast, high efficiency, sensitive and cost effective.This review describes the preparation technology, characterization methods, and related application characteristics of biomimetic imprinting affinity analysis recognition elements.The research status and application progress of different analysis methods, including fluorescence label biomimetic imprinting affinity analysis, enzyme label biomimetic imprinting affinity analysis, electrochemical biomimetic imprinting affinity analysis and biomimetic imprinting chemiluminescence affinity analysis, are summarized in food hazard analysis in recent years.The related contents provide new ideas for the research of new methods for food safety testing and technical supports for the detection of hazards in foods.

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