食品与发酵工业 >

2026 , Vol. 52 >Issue 3: 93 - 100

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.042644

研究报告

复合益生菌对大鼠龋齿的改善作用

  • 李刘若兰 ,
  • 喻喜华 ,
  • 王一 ,
  • 任玉 ,
  • 张秋香 ,
  • 崔树茂
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  • 1(国药控股星鲨制药(厦门)有限公司,福建 厦门,361026)
    2(厦门市母婴健康营养产品重点实验室,福建 厦门,361005)
    3(江南大学 食品学院,江苏 无锡,214122)
第一作者:硕士,助理工程师(崔树茂研究员为通信作者,E-mail:cuishumao@jiangnan.edu.cn)

收稿日期: 2025-03-07

  修回日期: 2025-06-03

  网络出版日期: 2026-03-13

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The improvement effect of composite probiotics on rat dental caries

  • LI Liuruolan ,
  • YU Xihua ,
  • WANG Yi ,
  • REN Yu ,
  • ZHANG Qiuxiang ,
  • CUI Shumao
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  • 1(Sinopharm Xingsha Pharmaceuticals (Xiamen) Co.Ltd., Xiamen 361026, China)
    2(Xiamen Key Laboratory of Maternal and Infant Health and Nutrition Products, Xiamen 361005, China)
    3(School of Food Science and Technology, Jiangnan University, Wuxi 214122, China)

Received date: 2025-03-07

  Revised date: 2025-06-03

  Online published: 2026-03-13

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摘要

龋齿是最常见的口腔疾病之一,主要由变异链球菌和白色念珠菌引起,研究证明抑制这2种致龋菌可以有效预防龋齿发生。为了评估由鼠李糖乳酪杆菌CCFM1070和唾液联合乳杆菌CCFM1215组成的复合益生菌对龋齿的改善作用,该研究通过建立大鼠龋齿模型,检测了益生菌干预后大鼠口腔中致龋菌数量变化、大鼠牙釉质体积大小及龋齿评分。结果发现,复合益生菌组可以显著降低大鼠口腔中变异链球菌的数量,干预4周后,由(5.55±0.06) lg CFU/mL降低到(4.03±0.14) lg CFU/mL(P<0.05),同时也显著抑制了白色念珠菌的数量(4.62±0.07)lg CFU/mL降低至(3.42±0.07) lg CFU/mL(P<0.05)。复合益生菌组大鼠的牙釉质体积由(1.75±0.17) mm3显著增加至(2.61±0.09) mm3(P<0.05),牙齿光滑面釉质龋(E级)、牙本质浅龋(Ds级)、牙本质中层龋(Dm级)和牙本质深层龋(Dx级)龋齿评分均显著降低,此外牙齿窝沟面Dm级龋齿评分由(6.67±1.33)显著降低至(0.67±0.42)(P<0.01)。以上结果说明本复合益生菌可以有效改善龋齿。

关键词： 益生菌; 大鼠龋齿模型; 变异链球菌; 白色念珠菌; 龋齿评分

本文引用格式

李刘若兰 , 喻喜华 , 王一 , 任玉 , 张秋香 , 崔树茂 . 复合益生菌对大鼠龋齿的改善作用[J]. 食品与发酵工业, 2026 , 52(3) : 93 -100 . DOI: 10.13995/j.cnki.11-1802/ts.042644

Abstract

Dental caries is one of the most common oral diseases, mainly caused by Streptococcus mutans and Candida albicans.Studies have proved that inhibiting these two cariogenic bacteria can effectively prevent the occurrence of dental caries.To evaluate the efficacy of a composite probiotics formulation containing Lacticaseibacillus rhamnosus CCFM1070 and Ligilactobacillus salivarius CCFM1215 in improving dental caries, a rat dental caries model was established.Changes in oral cariogenic microbial counts, enamel volume, and caries scores were analyzed after probiotic intervention.Results showed that the composite probiotics group significantly reduced the counts of S.mutans in the oral cavity of rats.After 4 weeks of intervention, the count decreased from (5.55±0.06) lg CFU/mL to (4.03±0.14) lg CFU/mL (P<0.05).Additionally, the composite probiotics markedly suppressed C.albicans levels (4.62±0.07) lg CFU/mL to (3.42±0.07) lg CFU/mL(P<0.05).The enamel volume of rats in the composite probiotics group was significantly increased from (1.75±0.17) mm3 to (2.61±0.09) mm3 (P<0.05).The enamel (E), slight dentinal (Ds), moderate dentinal (Dm), and extensive dentinal (Dx) caries scores of the smooth surface of teeth were significantly reduced.Additionally, the Dm-level caries score of the occlusal surface of teeth was significantly reduced from (6.67±1.33) to (0.67±0.42) (P<0.01).The above results indicate that this composite probiotics can effectively improve dental caries.

Key words： probiotics; rat dental caries model; Streptococcus mutans; Candida albicans; caries scores

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