食品与发酵工业 >

2026 , Vol. 52 >Issue 3: 166 - 175

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.042396

研究报告

基于微宇宙系统探究碳源类型对人工窖泥微生物群落结构及其代谢的影响

  • 朱性雪 ,
  • 唐云 ,
  • 尉军强 ,
  • 林一心 ,
  • 任聪 ,
  • 徐岩
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  • 1(江南大学 生物工程学院,酿造微生物学与应用酶学实验室,江苏 无锡,214122)
    2(金徽酒股份有限公司,甘肃 陇南,742300)
    3(江南大学,中国轻工业微生物组学与生态酿造技术重点实验室,江苏 无锡,214122)
第一作者:硕士研究生(任聪副研究员为通信作者,E-mail:congren@jiangnan.edu.cn)

收稿日期: 2025-02-17

  修回日期: 2025-06-10

  网络出版日期: 2026-03-13

基金资助

国家自然科学基金项目(32172177)

收起

Effects of carbon source types on microbial community structure and metabolism in artificial pit mud using microcosm system

  • ZHU Xingxue ,
  • TANG Yun ,
  • WEI Junqiang ,
  • LIN Yixin ,
  • REN Cong ,
  • XU Yan
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  • 1(Laboratory of Brewing Microbiology and Applied Enzymology, School of Biological Engineering, Jiangnan University, Wuxi 214122, China)
    2(Jinhui Liquor Co.Ltd., Longnan 742300, China)
    3(Eco-brewing Technology for Light Industry, Jiangnan University, Wuxi 214122, China)

Received date: 2025-02-17

  Revised date: 2025-06-10

  Online published: 2026-03-13

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摘要

以老窖泥为种子源制备新窖泥过程中微生物群落结构变化受到碳源种类影响的机理尚不清楚。该研究目的在于探索葡萄糖和乳酸对窖泥微生物菌群重构过程中微生物代谢和群落结构的影响。该研究采用厌氧微宇宙系统,在严格厌氧条件下进行窖泥培养,测定底物与代谢产物变化规律,运用扩增子测序技术分析微生物群落的动态变化特征。结果表明,模拟发酵中产己酸微生物相对丰度降低,但可正常代谢且己酸含量达到(9.52±0.23) g/kg。乳酸是窖泥微生物产己酸的重要碳源,而葡萄糖的添加减缓了短、中链脂肪酸的生成速率。窖泥制作过程不可避免出现乳酸菌大量生长现象。甲烷杆菌纲的相对丰度虽未出现较大幅度变化,但较大地压缩了拟杆菌纲和甲烷微菌纲的相对丰度。综上所述,该研究为进一步优化人工窖泥的制备过程提供了理论支持和实践依据。

关键词： 窖泥; 微宇宙系统; 己酸; 产己酸细菌; 短中链脂肪酸降解菌群

本文引用格式

朱性雪 , 唐云 , 尉军强 , 林一心 , 任聪 , 徐岩 . 基于微宇宙系统探究碳源类型对人工窖泥微生物群落结构及其代谢的影响[J]. 食品与发酵工业, 2026 , 52(3) : 166 -175 . DOI: 10.13995/j.cnki.11-1802/ts.042396

Abstract

The mechanism underlying how the types of carbon sources influence changes in microbial community structure during the preparation of artificial pit mud using aged pit mud as a seed source remains unclear.This study investigated the effects of glucose and lactate on microbial metabolism and community structure during the restructuring process of pit mud microbiota.An anaerobic microcosm system was employed to cultivate pit mud under strictly anaerobic condition.The dynamics of substrates and metabolites were determined, and amplicon sequencing technology was used to analyze the dynamic characteristics of microbial communities.The results showed that although the relative abundance of caproate-producing bacteria in simulated fermentation decreased, they still remained capability of normal metabolism and achieved caproate content of (9.52±0.23) g/kg.Lactate was an important carbon source for pit mud bacteria to produce caproate, whereas, the addition of glucose slowed down the production rate of short- and medium-chain fatty acids.The process of new-making pit mud inevitably led to the massive growth of lactic acid bacteria.Although, the relative abundance of Methanobacteria did not exhibit a significant change, yet it substantially suppressed the relative abundances of Bacteroidia and Methanomicrobia.Based on these results, this study provides theoretical support and practical basis for further optimizing the preparation of artificial pit mud.

Key words： pit mud; microcosm system; caproate; caproate-producing bacteria; short- and medium- chain fatty acid-degrading microorganisms

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