食品与发酵工业 >

2026 , Vol. 52 >Issue 4: 226 - 233

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.043333

研究报告

羊肚菌多糖对秀丽隐杆线虫的抗衰老活性及机制初探

  • 朱旋 ,
  • 孙海燕 ,
  • 袁必华 ,
  • 柳青 ,
  • 李晶 ,
  • 田芸
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  • (陕西理工大学 生物科学与工程学院,陕西 汉中,723000)
第一作者:硕士研究生(孙海燕教授为通信作者,E-mail:diyson2008@163.com)

收稿日期: 2025-05-20

  修回日期: 2025-06-30

  网络出版日期: 2026-03-17

基金资助

陕西省科技厅自然科学基金项目(2022JM-137)

收起

Preliminary study on anti-aging activity and mechanism of Morchella esculenta polysaccharide in Caenorhabditis elegans

  • ZHU Xuan ,
  • SUN Haiyan ,
  • YUAN Bihua ,
  • LIU Qing ,
  • LI Jing ,
  • TIAN Yun
Expand
  • (School of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723000, China)

Received date: 2025-05-20

  Revised date: 2025-06-30

  Online published: 2026-03-17

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摘要

为了深入挖掘羊肚菌多糖的高附加值,通过喂食不同质量浓度的羊肚菌多糖,对秀丽隐杆线虫寿命、体长、运动能力、生殖能力、抗应激能力、总超氧化物歧化酶(total superoxide dismutase,T-SOD)活力、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-PX)活力、丙二醛(malondialdehyde,MDA)含量、活性氧(reactive oxygen species,ROS)水平及DA1116(eat-2)、CF1903(glp-1)、CB1876(clk-1)、CB1370(daf-2)4种基因缺陷型线虫的氧化应激生存率的监测,评价羊肚菌多糖的抗衰老活性和机制。结果表明,与空白对照组相比,羊肚菌多糖处理组线虫的平均寿命最高延长了15.86%;第14天时,羊肚菌多糖处理组线虫C类运动最高降低了25.36%;线虫产卵量无显著变化;线虫体长在生长发育阶段无显著差异,第14天时25、12.5 mg/mL羊肚菌多糖组体长分别显著降低了5.2%和6.6%(P<0.05);12.5 mg/mL 羊肚菌多糖处理组的抗热应激能力显著提升;羊肚菌多糖处理后胰岛素信号基因缺陷型线虫CB1370(daf-2)生存率显著降低。结果表明,喂食羊肚菌多糖对线虫具有显著的抗衰老活性,为未来深入研究和开发提供了基础。

关键词： 羊肚菌多糖; 秀丽隐杆线虫; 抗衰老; 抗氧化

本文引用格式

朱旋 , 孙海燕 , 袁必华 , 柳青 , 李晶 , 田芸 . 羊肚菌多糖对秀丽隐杆线虫的抗衰老活性及机制初探[J]. 食品与发酵工业, 2026 , 52(4) : 226 -233 . DOI: 10.13995/j.cnki.11-1802/ts.043333

Abstract

To investigate the high added-value of Morchella polysaccharides and elucidate their anti-aging mechanisms, this study evaluated the effects of feeding Caenorhabditis elegans with different concentrations of Morchella polysaccharides lifespan, body length, motor ability, reproductive capacity, stress resistance, activities of total superoxide dismutase (T-SOD) and glutathione peroxidase (GSH-PX), contents of malondialdehyde (MDA) and reactive oxygen species (ROS), and oxidative stress survival rates in four gene-deficient strains, including DA1116 (eat-2), CF1903 (glp-1), CB1876 (clk-1), and CB1370 (daf-2).Results showed that compared with the blank control, Morchella polysaccharides significantly prolonged the average lifespan of C.elegans by up to 15.86%.On day 14, category C motor activity in Morchella polysaccharides-treated groups was reduced by 25.36% at maximum, while egg-laying capacity showed no significant changes.Body length exhibited no differences during the growth stage, but at day 14.25 mg/mL and 12.5 mg/mL Morchella polysaccharides significantly decreased body length by 5.2% and 6.6% (P<0.05), respectively.The 12.5 mg/mL Morchella polysaccharides group showed significantly enhanced heat stress resistance.Notably, treatment with Morchella polysaccharides reduced the oxidative stress survival rate of the insulin signalling-deficient strain CB1370 (daf-2), suggesting a potential role in insulin-like signalling pathways.Biochemical analyses revealed that Morchella polysaccharides modulated antioxidant enzyme activities (T-SOD, GSH-PX) and reduced ROS/MDA levels in a concentration-dependent manner, indicating their scavenging effects on reactive oxygen species.Genetic studies further demonstrated that the anti-aging effects of Morchella polysaccharides were partially mediated through pathways related to aging, such as food intake DA1116 (eat-2), germline development CF1903 (glp-1), mitochondrial function CB1876 (clk-1), and insulin signalling CB1370 (daf-2).Collectively, these findings highlight the significant anti-aging potential of Morchella polysaccharides in C.elegans, providing a mechanistic foundation for further research and development of Morchella polysaccharide-based functional foods or nutraceuticals targeting aging-related diseases.

Key words： Morchella polysaccharide; Caenorhabditis elegans; anti-aging; antioxidation

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