食品与发酵工业 >

2026 , Vol. 52 >Issue 4: 287 - 294

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.042988

研究报告

不同提取方法的桑黄多糖对果蝇的抗衰老作用

  • 朱蕴兰 ,
  • 陈安徽 ,
  • 邵颖 ,
  • 范晓博 ,
  • 郑义 ,
  • 闵庆友 ,
  • 王琪
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  • 1(徐州工程学院 食品与生物工程学院,江苏 徐州,221018)
    2(江苏省食品资源开发与质量安全重点建设实验室,江苏 徐州,221018)
    3(徐州工程学院 免疫药物分子遗传研究院,江苏 徐州,221018)
    4(江苏莫小希食品研究院有限公司,江苏,徐州,221100)
    5(徐州工程学院 师范学院,江苏 徐州,221018)
第一作者:硕士,教授(通信作者,E-mail:zhuyl@xzit.edu.cn)

收稿日期: 2025-04-11

  修回日期: 2025-07-05

  网络出版日期: 2026-03-17

基金资助

江苏省科技计划项目(BY20231311)

收起

Anti-aging effects of Phellinus igniarius polysaccharides extracted by different methods on Drosophila melanogaster

  • ZHU Yunlan ,
  • CHEN Anhui ,
  • SHAO Ying ,
  • FAN Xiaobo ,
  • ZHENG Yi ,
  • MIN Qingyou ,
  • WANG Qi
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  • 1(School of Food and Biological Engineering, Xuzhou University of Technology, Xuzhou 221018, China)
    2(Jiangsu Key Construction Laboratory of Food Resource Development and Quality Safety, Xuzhou 221018, China)
    3(Research Center of Immunopharmacology and Cytogenetics, Xuzhou University of Technology, Xuzhou 221018, China)
    4(Jiangsu Moxiaoxi Food Research Institute Co.Ltd., Xuzhou 221100, China)
    5(Normal College, Xuzhou University of Technology, Xuzhou 221018, China)

Received date: 2025-04-11

  Revised date: 2025-07-05

  Online published: 2026-03-17

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摘要

桑黄多糖具有抗氧化、抗衰老活性,但其活性因提取方法而异。为研究不同方法提取的桑黄多糖的抗衰老能力,该研究以黑腹果蝇为模型,将其随机分为雌雄空白对照组(A)、酶法多糖组(B)、微波法多糖组(C)与超声协同纤维素酶法多糖组(D)。通过测定果蝇组织内超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)、总抗氧化能力(total antioxidant capacity,T-AOC)、丙二醛(malondialdehyde,MDA)含量等指标,以及果蝇平均寿命、半数死亡时间和最高寿命,对多糖抗衰老能力进行评价。结果显示,在实验浓度下,B、C、D组多糖均可显著提升果蝇SOD和CAT活性,增强T-AOC,降低MDA含量,延长果蝇寿命(P＜0.01)。其中,D组效果最佳。在雌性果蝇中,D组SOD、CAT活性及T-AOC 较A组分别提升12.68%、87.32%、108.12%,MDA含量降低39.71%,3项寿命指标分别增加18.09%、19.30%、10.27%。雄性果蝇中,D组对应酶活性和T-AOC较A组分别提升23.46%、49.7%、59.64%,MDA含量降低41.04%,3项寿命指标分别增加16.42%、14.00%、7.19%。3种提取方法所得桑黄多糖均有抗氧化和延缓果蝇衰老的作用,超声协同纤维素酶法是较优提取方法,该研究为桑黄多糖开发提供了理论依据。

关键词： 桑黄; 多糖; 提取方法; 抗氧化; 抗衰老

本文引用格式

朱蕴兰 , 陈安徽 , 邵颖 , 范晓博 , 郑义 , 闵庆友 , 王琪 . 不同提取方法的桑黄多糖对果蝇的抗衰老作用[J]. 食品与发酵工业, 2026 , 52(4) : 287 -294 . DOI: 10.13995/j.cnki.11-1802/ts.042988

Abstract

Phellinus igniarius polysaccharides have antioxidant and anti-aging activity, but their activity varies depending on the extraction method.To study the anti-aging ability of P.igniarius polysaccharides extracted by different methods, in this study, Drosophila melanogaster was used as the model and randomly divided into male and female blank control group A, enzymatic polysaccharide group B, microwave polysaccharide group C, and ultrasound combined cellulase polysaccharide group D.The anti-aging of polysaccharides was evaluated by determining indicators such as superoxide dismutase (SOD), catalase (CAT), total antioxidant capacity (T-AOC), and malondialdehyde (MDA) content in the D.melanogaster tissues, as well as the average lifespan, half of death time, and maximum lifespan of the D.melanogaster.Results showed that at the experimental concentration, polysaccharides in groups B, C, and D could significantly increase the activities of SOD and CAT in D.melanogaster, enhance T-AOC, reduce the content of MDA, and prolong the lifespan of D.melanogaster (P<0.01).Among them, group D had the best effect.Among the female D.melanogaster, the activities of SOD and CAT and T-AOC in group D increased by 12.68%, 87.32%, and 108.12% respectively compared with group A, the content of MDA decreased by 39.71%, and the three lifespan indicators increased by 18.09%, 19.30%, and 10.27% respectively.Among the male D.melanogaster, the activities of SOD and CAT and T-AOC in group D increased by 23.46%, 49.7%, and 59.64% respectively compared with group A, the MDA content decreased by 41.04%, and the three lifespan indicators increased by 16.42%, 14.00%, and 7.19% respectively.The polysaccharides from P.igniarius obtained by the three extraction methods all have the effects of an antioxidant and delaying the aging of D.melanogaster. The ultrasound-combined cellulase method is a better extraction method.This study provides a theoretical basis for the development of P.igniarius polysaccharides.

Key words： Phellinus igniarius; polysaccharide; extraction method; antioxidant; anti-aging

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